Dr. Nancy Pleshko is a Professor in the Department of Bioengineering at Temple University, Philadelphia, PA. Dr. Pleshko is recognized for her research on assessment of tissues at the molecular, cellular, and structural level through application of state-of-the-art vibrational spectroscopy. She has substantial expertise in basic and translational research in connective tissue pathophysiology and orthopedics, including osteoporosis and other bone pathologies, and osteoarthritis and cartilage repair. She has over 90 publications, and has received over $5 million in federal funding.

Research Interests

  • Molecular Imaging of Tissues & Cells
  • Spectroscopic Analysis Techniques for Cartilage and Bone Pathologies
  • Tissue Engineering
  • Contribution of Molecular Alterations in Connective Tissue on Mechanical Behavior
  • MicroCT & Magnetic Resonance Imaging of Connective Tissues

Courses Taught




BIOE 0856

Ethical Issues in Biomedical Science, Engineering and Technology


BIOE 3001

Research Design and Methods in Bioengineering


ENGG 3385

Diamond Peer Teachers - Internship I


Selected Publications

  • Jones, B.C., Jia, S., Lee, H., Feng, A., Shetye, S.S., Batzdorf, A., Shapira, N., Noël, P.B., Pleshko, N., & Rajapakse, C.S. (2021). MRI-derived porosity index is associated with whole-bone stiffness and mineral density in human cadaveric femora. Bone, 143. doi: 10.1016/j.bone.2020.115774

  • Ailavajhala, R., Querido, W., Rajapakse, C.S., & Pleshko, N. (2020). Near infrared spectroscopic assessment of loosely and tightly bound cortical bone water. Analyst, 145(10), pp. 3713-3724. doi: 10.1039/c9an02491c

  • Shanas, N., Querido, W., Dumont, A., Yonko, E., Carter, E., Ok, J., Karchner, J.P., Barbe, M.F., Ali, S., Patil, C., Raggio, C., & Pleshko, N. (2020). Clinical application of near infrared fiber optic spectroscopy for noninvasive bone assessment. Journal of Biophotonics, 13(4). doi: 10.1002/jbio.201960172

  • Kandel, S., Querido, W., Falcon, J.M., Reiners, D.J., & Pleshko, N. (2020). Approaches for in situ monitoring of matrix development in hydrogel-based engineered cartilage. Tissue Engineering - Part C: Methods, 26(4), pp. 225-238. doi: 10.1089/ten.tec.2020.0014

  • Querido, W., Shanas, N., Bookbinder, S., Oliveira-Nunes, M.C., Krynska, B., & Pleshko, N. (2020). Fourier transform infrared spectroscopy of developing bone mineral: From amorphous precursor to mature crystal. Analyst, 145(3), pp. 764-776. doi: 10.1039/c9an01588d

  • Falcon, J.M., Chirman, D., Veneziale, A., Morman, J., Bolten, K., Kandel, S., Querido, W., Freeman, T., & Pleshko, N. (2020). DMOG Negatively Impacts Tissue Engineered Cartilage Development. Cartilage. doi: 10.1177/1947603520967060

  • Ailavajhala, R., Oswald, J., Rajapakse, C.S., & Pleshko, N. (2019). Environmentally-Controlled Near Infrared Spectroscopic Imaging of Bone Water. Scientific Reports, 9(1). doi: 10.1038/s41598-019-45897-3

  • Hong, A.L., Ispiryan, M., Padalkar, M.V., Jones, B.C., Batzdorf, A.S., Shetye, S.S., Pleshko, N., & Rajapakse, C.S. (2019). MRI-derived bone porosity index correlates to bone composition and mechanical stiffness. Bone Reports, 11. doi: 10.1016/j.bonr.2019.100213

  • Karchner, J.P., Yousefi, F., Bitman, S.R., Darvish, K., & Pleshko, N. (2019). Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties. Cartilage, 10(4), pp. 480-490. doi: 10.1177/1947603518764269

  • Karchner, J.P., Querido, W., Kandel, S., & Pleshko, N. (2019). Spatial correlation of native and engineered cartilage components at micron resolution. Annals of the New York Academy of Sciences, 1442(1), pp. 104-117. doi: 10.1111/nyas.13934

  • Tint, D., Stabler, C.T., Hanifi, A., Yousefi, F., Linkov, G., Hy, K., Soliman, A., & Pleshko, N. (2019). Spectroscopic Analysis of Human Tracheal Tissue during Decellularization. Otolaryngology - Head and Neck Surgery (United States), 160(2), pp. 302-309. doi: 10.1177/0194599818806271

  • Jongwattanapisan, P., Terajima, M., Miguez, P.A., Querido, W., Nagaoka, H., Sumida, N., Gurysh, E.G., Ainslie, K.M., Pleshko, N., Perera, L., & Yamauchi, M. (2018). Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function. Scientific Reports, 8(1). doi: 10.1038/s41598-018-25279-x

  • Querido, W., Ailavajhala, R., Padalkar, M., & Pleshko, N. (2018). Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy. Applied Spectroscopy, 72(11), pp. 1581-1593. doi: 10.1177/0003702818789165

  • Wischmann, J., Lenze, F., Thiel, A., Bookbinder, S., Querido, W., Schmidt, O., Burgkart, R., Eisenhart-Rothe, R.v., Richter, G., Pleshko, N., & Mayer-Kuckuk, P. (2018). Matrix mineralization controls gene expression in osteoblastic cells. Experimental Cell Research, 372(1), pp. 25-34. doi: 10.1016/j.yexcr.2018.09.005

  • Yousefi, F., Kandel, S., & Pleshko, N. (2018). Infrared Spectroscopic Quantification of Methacrylation of Hyaluronic Acid: A Scaffold for Tissue Engineering Applications. Applied Spectroscopy, 72(10), pp. 1455-1466. doi: 10.1177/0003702818785353

  • Choi, H., Tessier, S., Silagi, E.S., Kyada, R., Yousefi, F., Pleshko, N., Shapiro, I.M., & Risbud, M.V. (2018). A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis. Matrix Biology, 70, pp. 102-122. doi: 10.1016/j.matbio.2018.03.019

  • Linkov, G., Hanifi, A., Yousefi, F., Tint, D., Bolla, S., Marchetti, N., Soliman, A., & Pleshko, N. (2018). Compositional Assessment of Human Tracheal Cartilage by Infrared Spectroscopy. Otolaryngology - Head and Neck Surgery (United States), 158(4), pp. 688-694. doi: 10.1177/0194599817752310

  • Yousefi, F., Kim, M., Nahri, S.Y., Mauck, R.L., & Pleshko, N. (2018). Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs. Tissue Engineering - Part A, 24(1-2), pp. 106-116. doi: 10.1089/ten.tea.2017.0035

  • Querido, W., Falcon, J.M., Kandel, S., & Pleshko, N. (2017). Vibrational spectroscopy and imaging: Applications for tissue engineering. Analyst, 142(21), pp. 4005-4017. doi: 10.1039/c7an01055a

  • Qu, D., Subramony, S.D., Boskey, A.L., Pleshko, N., Doty, S.B., & Lu, H.H. (2017). Compositional mapping of the mature anterior cruciate ligament-to-bone insertion. Journal of Orthopaedic Research, 35(11), pp. 2513-2523. doi: 10.1002/jor.23539

  • Rajapakse, C.S., Padalkar, M.V., Yang, H.J., Ispiryan, M., & Pleshko, N. (2017). Non-destructive NIR spectral imaging assessment of bone water: Comparison to MRI measurements. Bone, 103, pp. 116-124. doi: 10.1016/j.bone.2017.06.015

  • Doty, S.B., Bostrom, M.P., Bockman, R.S., Meulen, M.C.H.v.d., & Pleshko, N. (2017). Adele Ludin Boskey, PhD IN MEMORIAM. JOURNAL of BONE and MINERAL RESEARCH, 32(8), pp. 1597-1598. doi: 10.1002/jbmr.3187

  • Hanifi, A., Palukuru, U., McGoverin, C., Shockley, M., Frank, E., Grodzinsky, A., Spencer, R.G., & Pleshko, N. (2017). Near infrared spectroscopic assessment of developing engineered tissues: correlations with compositional and mechanical properties. Analyst, 142(8), pp. 1320-1332. doi: 10.1039/c6an02167k

  • Cheheltani, R., Pichamuthu, J.E., Rao, J., Weinbaum, J.S., Kiani, M.F., Vorp, D.A., & Pleshko, N. (2017). Fourier Transform Infrared Spectroscopic Imaging-Derived Collagen Content and Maturity Correlates with Stress in the Aortic Wall of Abdominal Aortic Aneurysm Patients. Cardiovascular Engineering and Technology, 8(1), pp. 70-80. doi: 10.1007/s13239-016-0289-3

  • Spencer, R.G., Cortese, B.D., Lukas, V.A., & Pleshko, N. (2017). Point Estimates of Test Sensitivity and Specificity from Sample Means and Variances. American Statistician, 71(1), pp. 81-87. doi: 10.1080/00031305.2016.1239589

  • Wolfson, M.R., Pleshko, N., Darvish, K.K., Baker, S.T., Wu, J., Kyada, R., Nelson, C., Fukuda, S., Enkhbaatar, P., Florova, G., Komissarov, A.A., Idell, S., & Shaffer, T.H. (2017). Differential Impact Of Fibrinolysins On Airway Cast Composition And Mechanical Properties Assessed By A Novel Infrared Imaging And Microindentation Approach. AMERICAN JOURNAL of RESPIRATORY and CRITICAL CARE MEDICINE, 195. Retrieved from

  • Marcott, C., Padalkar, M., & Pleshko, N. (2017). 3.23 Infrared and raman microscopy and imaging of biomaterials at the micro and nano scale. In Comprehensive Biomaterials II (pp. 498-518). doi: 10.1016/B978-0-12-803581-8.10183-3

  • Gangolli, R.A., Devlin, S.M., Ailavajhala, R., Hanifi, A., Pleshko, N., Lelkes, P.I., & Yang, M. (2016). Biomimetic Scaffold to Regenerate the Pulp Dentin Complex. TISSUE ENGINEERING PART A, 22, pp. S72-S72. Retrieved from

  • Raggio, C.L., Pleshko, N., & Boskey, A.L. (2016). The Effect of Stontium Ranelate on Fracture Reduction in Osteogenesis Imperfecta is Comparable to Recent Bisphosphonate Data. Journal of Bone and Mineral Research, 31(11), p. 2065. doi: 10.1002/jbmr.2976

  • Eisenhauer, P., Chernets, N., Song, Y., Dobrynin, D., Pleshko, N., Steinbeck, M.J., & Freeman, T.A. (2016). Chemical modification of extracellular matrix by cold atmospheric plasma-generated reactive species affects chondrogenesis and bone formation. Journal of Tissue Engineering and Regenerative Medicine, 10(9), pp. 772-782. doi: 10.1002/term.2224

  • Palukuru, U.P., Hanifi, A., McGoverin, C.M., Devlin, S., Lelkes, P.I., & Pleshko, N. (2016). Near infrared spectroscopic imaging assessment of cartilage composition: Validation with mid infrared imaging spectroscopy. Analytica Chimica Acta, 926, pp. 79-87. doi: 10.1016/j.aca.2016.04.031

  • Ashinsky, B.G., Fishbein, K.W., Carter, E.M., Lin, P.C., Pleshko, N., Raggio, C.L., & Spencer, R.G. (2016). Multiparametric classification of skin from osteogenesis imperfecta patients and controls by quantitative magnetic resonance microimaging. PLoS ONE, 11(7). doi: 10.1371/journal.pone.0157891

  • McGoverin, C.M., Hanifi, A., Palukuru, U.P., Yousefi, F., Glenn, P., Shockley, M., Spencer, R.G., & Pleshko, N. (2016). Nondestructive Assessment of Engineered Cartilage Composition by Near Infrared Spectroscopy. Annals of Biomedical Engineering, 44(3), pp. 680-692. doi: 10.1007/s10439-015-1536-8

  • Bozkurt, O., Bilgin, M.D., Evis, Z., Pleshko, N., & Severcan, F. (2016). Early Alterations in Bone Characteristics of Type i Diabetic Rat Femur: A Fourier Transform Infrared (FT-IR) Imaging Study. Applied Spectroscopy, 70(12), pp. 2005-2015. doi: 10.1177/0003702816671059