Dr. Nancy Pleshko is a Professor in the Department of Bioengineering at Temple University, Philadelphia, PA. Dr. Pleshko is recognized for her research on assessment of tissues at the molecular, cellular, and structural level through application of state-of-the-art vibrational spectroscopy. She has substantial expertise in basic and translational research in connective tissue pathophysiology and orthopedics, including osteoporosis and other bone pathologies, and osteoarthritis and cartilage repair. She has over 90 publications, and has received over $5 million in federal funding.

Research Interests

  • Molecular Imaging of Tissues & Cells
  • Spectroscopic Analysis Techniques for Cartilage and Bone Pathologies
  • Tissue Engineering
  • Contribution of Molecular Alterations in Connective Tissue on Mechanical Behavior
  • MicroCT & Magnetic Resonance Imaging of Connective Tissues

Courses Taught




BIOE 0856

Ethical Issues in Biomedical Science, Engineering and Technology


BIOE 0956

Honors Ethical Issues in Biomedical Science, Engineering and Technology


Selected Publications

  • Reiner, E., Weston, F., Pleshko, N., & Querido, W. (2023). Application of Optical Photothermal Infrared (O-PTIR) Spectroscopy for Assessment of Bone Composition at the Submicron Scale. Appl Spectrosc, 77(11), pp. 1311-1324. United States. doi: 10.1177/00037028231201427

  • Gidde, S.T.R., Acharya, S.R., Kandel, S., Pleshko, N., & Hutapea, P. (2022). Assessment of tissue damage from mosquito-inspired surgical needle. Minim Invasive Ther Allied Technol, 31(7), pp. 1112-1121. England. doi: 10.1080/13645706.2022.2051718

  • Kim, M., Koyama, E., Saunders, C.M., Querido, W., Pleshko, N., & Pacifici, M. (2022). Synovial joint cavitation initiates with microcavities in interzone and is coupled to skeletal flexion and elongation in developing mouse embryo limbs. Biol Open, 11(6). England. doi: 10.1242/bio.059381

  • Querido, W., Zouaghi, S., Padalkar, M., Morman, J., Falcon, J., Kandel, S., & Pleshko, N. (2022). Nondestructive assessment of tissue engineered cartilage based on biochemical markers in cell culture media: application of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Analyst, 147(8), pp. 1730-1741. England. doi: 10.1039/d1an02351a

  • Shanas, N., Querido, W., Oswald, J., Jepsen, K., Carter, E., Raggio, C., & Pleshko, N. (2022). Infrared Spectroscopy-Determined Bone Compositional Changes Associated with Anti-Resorptive Treatment of the oim/oim Mouse Model of Osteogenesis Imperfecta. Appl Spectrosc, 76(4), pp. 416-427. United States. doi: 10.1177/00037028211055477

  • Querido, W., Shanas, N., Radway, A.P., Block, J., Jones, B.C., Ispiryan, M., Shetye, S.S., Hast, M.W., Zhao, H., Rajapakse, C.S., & Pleshko, N. (2022). The Contribution of Tissue-Level Bone Composition to Strength at the Femoral Neck. JOURNAL of BONE and MINERAL RESEARCH, 37, pp. 277-278. Retrieved from

  • Kandel, S., Querido, W., Falcon, J.M., Zlotnick, H.M., Locke, R.C., Stoeckl, B., Patel, J.M., Patil, C.A., Mauck, R.L., & Pleshko, N. (2022). In Situ Assessment of Porcine Osteochondral Repair Tissue in the Visible-Near Infrared Spectral Region. Front Bioeng Biotechnol, 10, p. 885369. Switzerland. doi: 10.3389/fbioe.2022.885369

  • Falcon, J.M., Chirman, D., Veneziale, A., Morman, J., Bolten, K., Kandel, S., Querido, W., Freeman, T., & Pleshko, N. (2021). DMOG Negatively Impacts Tissue Engineered Cartilage Development. Cartilage, 13(2_suppl), pp. 722S-733S. United States. doi: 10.1177/1947603520967060

  • Miguez, P.A., Tuin, S.A., Robinson, A.G., Belcher, J., Jongwattanapisan, P., Perley, K., Gonҫalves, V.d.e.P., Hanifi, A., Pleshko, N., & Barton, E.R. (2021). Hesperidin Promotes Osteogenesis and Modulates Collagen Matrix Organization and Mineralization In Vitro and In Vivo. Int J Mol Sci, 22(6). Switzerland. doi: 10.3390/ijms22063223

  • Sanden, K.W., Böcker, U., Ofstad, R., Pedersen, M.E., Høst, V., Afseth, N.K., Rønning, S.B., & Pleshko, N. (2021). Characterization of Collagen Structure in Normal, Wooden Breast and Spaghetti Meat Chicken Fillets by FTIR Microspectroscopy and Histology. Foods, 10(3). Switzerland. doi: 10.3390/foods10030548

  • Querido, W., Kandel, S., & Pleshko, N. (2021). Applications of Vibrational Spectroscopy for Analysis of Connective Tissues. Molecules, 26(4). Switzerland. doi: 10.3390/molecules26040922

  • Jones, B.C., Jia, S., Lee, H., Feng, A., Shetye, S.S., Batzdorf, A., Shapira, N., Noël, P.B., Pleshko, N., & Rajapakse, C.S. (2021). MRI-derived porosity index is associated with whole-bone stiffness and mineral density in human cadaveric femora. Bone, 143, p. 115774. United States. doi: 10.1016/j.bone.2020.115774

  • Afara, I.O., Shaikh, R., Nippolainen, E., Querido, W., Torniainen, J., Sarin, J.K., Kandel, S., Pleshko, N., & Töyräs, J. (2021). Characterization of connective tissues using near-infrared spectroscopy and imaging. Nat Protoc, 16(2), pp. 1297-1329. England. doi: 10.1038/s41596-020-00468-z

  • Chirman, D. & Pleshko, N. (2021). Characterization of bacterial biofilm infections with Fourier transform infrared spectroscopy: a review. Applied Spectroscopy Reviews, 56(8-10), pp. 673-701. doi: 10.1080/05704928.2020.1864392

  • Ailavajhala, R., Querido, W., Rajapakse, C.S., & Pleshko, N. (2020). Near infrared spectroscopic assessment of loosely and tightly bound cortical bone water. Analyst, 145(10), pp. 3713-3724. England. doi: 10.1039/c9an02491c

  • Shanas, N., Querido, W., Dumont, A., Yonko, E., Carter, E., Ok, J., Karchner, J.P., Barbe, M.F., Ali, S., Patil, C., Raggio, C., & Pleshko, N. (2020). Clinical application of near infrared fiber optic spectroscopy for noninvasive bone assessment. J Biophotonics, 13(4), p. e201960172. Germany. doi: 10.1002/jbio.201960172

  • Kandel, S., Querido, W., Falcon, J.M., Reiners, D.J., & Pleshko, N. (2020). Approaches for In Situ Monitoring of Matrix Development in Hydrogel-Based Engineered Cartilage. Tissue Eng Part C Methods, 26(4), pp. 225-238. United States. doi: 10.1089/ten.TEC.2020.0014

  • Querido, W., Shanas, N., Bookbinder, S., Oliveira-Nunes, M.C., Krynska, B., & Pleshko, N. (2020). Fourier transform infrared spectroscopy of developing bone mineral: from amorphous precursor to mature crystal. Analyst, 145(3), pp. 764-776. England. doi: 10.1039/c9an01588d

  • Shanas, N., Querido, W., Pleshko, N., Yonko, E., Carter, E., & Raggio, C. (2019). Non-invasive near infrared spectroscopic method for bone quality assessment in osteogenesis imperfecta. JOURNAL of BONE and MINERAL RESEARCH, 34, pp. 190-191. Retrieved from

  • Shanas, N., Querido, W., Pleshko, N., Yonko, E., Carter, E., & Raggio, C. (2019). Non-invasive near infrared spectroscopic method for bone quality assessment in osteogenesis imperfecta. JOURNAL of BONE and MINERAL RESEARCH, 34, pp. 190-191. Retrieved from

  • Hong, A.L., Ispiryan, M., Padalkar, M.V., Jones, B.C., Batzdorf, A.S., Shetye, S.S., Pleshko, N., & Rajapakse, C.S. (2019). MRI-derived bone porosity index correlates to bone composition and mechanical stiffness. Bone Rep, 11, p. 100213. United States. doi: 10.1016/j.bonr.2019.100213

  • Karchner, J.P., Yousefi, F., Bitman, S.R., Darvish, K., & Pleshko, N. (2019). Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties. Cartilage, 10(4), pp. 480-490. United States. doi: 10.1177/1947603518764269

  • Ailavajhala, R., Oswald, J., Rajapakse, C.S., & Pleshko, N. (2019). Environmentally-Controlled Near Infrared Spectroscopic Imaging of Bone Water. Sci Rep, 9(1), p. 10199. England. doi: 10.1038/s41598-019-45897-3

  • Karchner, J.P., Querido, W., Kandel, S., & Pleshko, N. (2019). Spatial correlation of native and engineered cartilage components at micron resolution. Ann N Y Acad Sci, 1442(1), pp. 104-117. United States. doi: 10.1111/nyas.13934

  • Tint, D., Stabler, C.T., Hanifi, A., Yousefi, F., Linkov, G., Hy, K., Soliman, A.M.S., & Pleshko, N. (2019). Spectroscopic Analysis of Human Tracheal Tissue during Decellularization. Otolaryngol Head Neck Surg, 160(2), pp. 302-309. England. doi: 10.1177/0194599818806271

  • Wolfson, M.R., Pleshko, N., Darvish, K., Marcinkiewicz, M., Wu, J., Rakymzhan, A., Kyada, R., Enkhbaatar, P., Fukuda, S., Nelson, C., Williams, R.O., Komissarov, A., Florova, G., Shaffer, T.H., & Idell, S. (2019). Understanding Airway Casts Secondary to Inhalational Smoke-Induced Acute Lung Injury (ISALI): Independent Assessment Tools of Composition and Fibrinolysin Impact. AMERICAN JOURNAL of RESPIRATORY and CRITICAL CARE MEDICINE, 199. Retrieved from