Bojana Gligorijevic, PhD, graduated Chemistry at University of Belgrade with an honors thesis in analytical chemistry, isolating active compounds from endemic plants. At Georgetown University, Dr. Gligorijevic’s PhD chemistry thesis took a turn when she first saw a fluorescent microscope. She ended up developing new technologies for automated 4D imaging and analysis of malaria division and metabolic processes. During her postdoctoral training in cancer biology at Albert Einstein College of Medicine, she developed new tools for intravital imaging in mouse models and contributed to the current view of tumor cell intravasation mechanism in tumor microenvironment context.

Research Interests

  • Intravital imaging
  • Multiphoton Microscopy
  • Machine Learning Image Classification
  • Fluorescent Proteins
  • Cancer Complexity
  • Cell Decision-making
  • Extracellular Matrix Remodeling
  • Tumor Microenvironments
  • Mouse Models of Metastasis
  • Invadopodia
  • Motility & Invasion.

Courses Taught




BIOE 0856

Ethical Issues in Biomedical Science, Engineering and Technology


BIOE 5555

Biophotonics: Seeing is Believing


Selected Publications


  • Velazquez-Quesada, I., Allison, K., Alizadeh, V., Hesketh, N., Thomas, G., Tuzel, E., & Gligorijevic, B. (2024). DACIT: Device for Axon - Cancer cell Interaction Testing in 2D and 3D. doi: 10.1101/2024.02.26.582218

  • Kallogjerovic, S., Velázquez-Quesada, I., Hadap, R., & Gligorijevic, B. (2024). Retrograde tracing of breast cancer-associated sensory neurons. doi: 10.1101/2024.02.26.582088

  • Ogier, C., Solomon, A.M.C., Lu, Z., Recoules, L., Klochkova, A., Gabitova-Cornell, L., Bayarmagnai, B., Restifo, D., Surumbayeva, A., Vendramini-Costa, D.B., Deneka, A.Y., Francescone, R., Lilly, A.C., Sipman, A., Gardiner, J.C., Luong, T., Franco-Barraza, J., Ibeme, N., Cai, K.Q., Einarson, M.B., Nicolas, E., Efimov, A., Megill, E., Snyder, N.W., Bousquet, C., Cros, J., Zhou, Y., Golemis, E.A., Gligorijevic, B., Soboloff, J., Fuchs, S.Y., Cukierman, E., & Astsaturov, I. (2023). Trogocytosis of cancer-associated fibroblasts promotes pancreatic cancer growth and immune suppression via phospholipid scramblase anoctamin 6 (ANO6). BioRxiv. United States. doi: 10.1101/2023.09.15.557802

  • Perrin, L. & Gligorijevic, B. (2022). Proteolytic and mechanical remodeling of the extracellular matrix by invadopodia in cancer. Phys Biol, 20(1). England. doi: 10.1088/1478-3975/aca0d8

  • Perrin, L., Belova, E., Bayarmagnai, B., Tüzel, E., & Gligorijevic, B. (2022). Invadopodia enable cooperative invasion and metastasis of breast cancer cells. Commun Biol, 5(1), p. 758. England. doi: 10.1038/s42003-022-03642-z

  • Perrin, L., Bayarmagnai, B., Tuzel, E., & Gligorijevic, B. (2021). Invadopodia enable cooperative invasion and metastasis of breast cancer cells. doi: 10.21203/

  • Perrin, L., Belova, E., Bayarmagnai, B., Tüzel, E., & Gligorijevic, B. (2021). Invadopodia enable cooperative invasion and metastasis of breast cancer cells. doi: 10.1101/2021.02.13.431047

  • Perrin, L., Tucker, T., & Gligorijevic, B. (2021). Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model. J Vis Exp.(167). United States. doi: 10.3791/61902

  • Firestein, R., Marcinkiewicz, C., Nie, L., Chua, H.K., Quesada, I.V., Torelli, M., Sternberg, M., Gligorijevic, B., Shenderova, O., Schirhagl, R., & Feuerstein, G.Z. (2021). Pharmacodynamic Studies of Fluorescent Diamond Carriers of Doxorubicin in Liver Cancer Cells and Colorectal Cancer Organoids. Nanotechnol Sci Appl, 14, pp. 139-159. New Zealand. doi: 10.2147/NSA.S321725

  • Gligorijevic, B. (2020). Real-time, high-resolution microscopy of invasive cancer cells in the tumor microenvironment context. J Biomol Tech, 31(Suppl), p. S40. United States. Retrieved from

  • Perrin, L., Bayarmagnai, B., & Gligorijevic, B. (2020). Frontiers in Intravital Multiphoton Microscopy of Cancer. Cancer Rep (Hoboken), 3(1), p. e1192. United States. doi: 10.1002/cnr2.1192

  • Bayarmagnai, B., Perrin, L., Pourfarhangi, K.E., Graña, X., Tüzel, E., & Gligorijevic, B. (2019). Invadopodia-mediated ECM degradation is enhanced in the G1 phase of the cell cycle. J Cell Sci, 132(20). England. doi: 10.1242/jcs.227116

  • Pourfarhangi, K.E., Bergman, A., & Gligorijevic, B. (2019). Invadopodia Dynamics is Regulated by ECM Cross-Linking. BIOPHYSICAL JOURNAL, 116(3), pp. 414A-414A. Retrieved from

  • Pourfarhangi, K.E. & Gligorijevic, B. (2019). Machine Learning Classification of Cancer Cells Migration in 3D Multi-cue Microenvironments 1. 2018 IEEE Signal Processing in Medicine and Biology Symposium, SPMB 2018 - Proceedings. doi: 10.1109/SPMB.2018.8615608