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Research Capabilities

Chemistry | Screening | Biology

The Moulder Center for Drug Discovery Research and School of Pharmacy have four laboratories with approximately 8,000 square feet and 16 fume hoods dedicated to the synthesis and purification of organic molecules.  The chemistry laboratories are designed to handle mg to kg scale small molecule synthesis.  Parallel synthesis platforms are also available for the preparation of either diversity driven or focused libraries of 10s to 1000s of compounds on 10 - 100 mg scale.  The Moulder center labs and associated facilities are also suitable for large scale synthesis to meet the needs of PK studies, in vivo animal studies, and cGMP production.

In addition to the standard laboratory glassware and equipment (e.g. rotovaps, balances, vacuum pumps, etc.), the Moulder Center labs are equipped with:

• Gilson 215 semi-preparative HPLC system
• Gilson GX-281 preparative HPLC system
• Genevac EZ-2Evaporator
• CEM Explorer microwave
• Teledyne Isco Combiflash Rf system (2)
• Grace Reveleris HPLC chromatography system with ELSD detection
• Walk in fume hood capabilities for large scale chemistry

Specific Analytical Instrumentation and Equipment Includes:

• Nuclear Magnetic Resonance (NMR) Instruments:
• Bruker 400 MHz NMR (School of Pharmacy)
• Bruker 300, 400 MHz (College of Science and Technology
• GE 500 MHz (College of Science and Technology )
• Mass Spectroscopy:
• Agilent HPLC/MS ESI with PDA
• API 4000 LC/MS/MS
• FT-IR Perkin-Elmer Model 1625.
• X-ray Crystallography via the Dept. of Chemistry, U. of Penn.
• Shimadzu UPLC system LC-20AD suitable for HPLC and GPC.
• TA Instruments Q200 modulated differential scanning calorimeter with RCS cooling unit (range 90°C-400°C).
• Malvern ZS Nano particle size analyzer with zeta potential measurement.

Solid Phase Peptide Synthesis (SPPS) Capabilities:

Through collaboration with Sbarro Health Research Organization (SHRO) Moulder center scientists have access to Solid Phase Peptide Synthesis (SPPS ) which combines state-of-the-art equipment with the latest cutting edge methodology. Comprehensive expertise for peptide synthesis and purification, as well as identification and characterization of proteins from recombinant or biofluidic sources are available.  For addition information, please visit the Sbarro Health Research Organization web site (http://www.shro.org/SPPS/).

Screening Capabilities:

MCDDR maintains a 500 square feet facility equipped with tissue and cell culture capabllilles and two PerkinElmer Janus workstations coupled with a state-of-the-art EnVision Multilabel plate reader as well as capabilities to support assay development such as Alpha technology.

Biology Capabilities:

In vivo:

• Stereotaxic surgery (precise micro injection, sampling in brain regions)
• Micro dissection
• Microanalysis
• Seizure activity (vertebrates and invertebrates)
• Behavioral assessment
• Locomotor activity and behavioral sensitization
• Conditioned place preference
• Analgesia and tolerance
• Anxiety
• Depression
• Thermoregulation

Ex vivo:

• Radio ligand
• Homogenate binding
• Autoradiography
• Rubidium efflux
• Neurotransmitter release
• Receptor profiling
• Drug affinities
• Functional responses
• Subunit composition
• Quantification and distribution
• Protein and neuron characterization
• Western blotting
• Immunohistochemistry
• HPLC
• Synaptosomal preparations

Other Specialized Capabilities :

• Pharmacogenomic Analysis of SNP's
• Copy Number Variations
• Cell-based assays such as cytotoxicity, apoptosis, caspase activation, cell viability
• In-vitro metabolism
• Pre-clinical PK

 

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