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Human neurosphere under diff -CIII beta tubulin. Image courtesy of Servio Ramirez, PhD, Department of Pathology and Laboratory Medicine, TUSM

RESOURCES FOR INVESTIGATORS

RESEARCH FACILITIES: PROTEOMICS RESEARCH FACILITY

Temple Proteomics Research Facility provides accessible and affordable proteomics capability for biological and biomedical research. This research facility has state-of-the-art instrumentation, and is supported by staff with expertise in proteomics, tissue microanalysis and relevant bioinformatics. Services provided include expression profiles, protein identification, posttranslational modification, characterization isolation of individual cells from tissues, protein and peptide separations, capillary electrophoresis, and multidimensional protein identification technology (MudPIT).

 

In addition to providing traditional two dimensional gel electrophoresis for protein expression profiling, we have implemented mass spectroscopy-based proteomics techniques using Stable Isotope Labeling of Amino Acid in Cell Culture (SILAC) and Isotope-Coded Affinity Tag (ICAT). Using the combination of these technologies, it is now possible to obtain high fidelity data for biomarkers discovery, drug targets, as well as studies on pathogenesis of diseases.

 

The equipment for proteomics analysis includes:

  • Bruker autoflex mass TOF/TOF mass spectrometer
  • Bruker HCT ultra ion trap mass spectrometer coupled with Agillent nanoLC system
  • Ettan Maldi-TOF Pro
  • Shimadzu Xcise automated robotic system for gel processing
  • Applied Biosystem Voyager workstation MALDI-TOF mass spectrometer
  • Amersham Biosciences ETTAN DIGE 2D gel electrophoresis system and Multiphor 2d gel analysis system
  • Dedicated computer for differential expression analysis with Z3 software from Compugen
  • Dedicated server for mascot bioinformatics software
  • Beckman Coulter MDQ capillary system with laser induced florescence detector
  • Photodiode array detector and Finnigan LCQ mass spectrometer
  • Waters 2795 HPLC system complete with photodiode array detector, scanning florescence detector and dedicated computer configured with Empower software
  • Shimadzu complete HPLC 10AVP system with florescence detector and photodiode array detector
  • Arcturus Veritas laser capture microdissection system with dedicated computer configured with Vertitas software