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Huichen Wang, PhD

Assistant Professor, Neuroscience

Assistant Professor, Neurovirology

Telephone:  215-204-9687

Fax:  215-204-0679

Email: hcwang@temple.edu

Department of Neuroscience

Center for Neurovirology

VMD, Veterinary Medicine - 1984

Chinese Veterinary Medical College

Changchun, China

 

MS, Virology - 1987

Institute for Virology

Changchun, China

 

PhD, Medical Genetics - 1992

Chinese Academy of Medical Science

Beijing, China

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Mechanism involved in the maintenance of chromosome integrity in normal and disease stages; fidelity of DNA repair in neural cells.

Research Summary
Our laboratory is focused on understanding the mechanism of DNA double strand break (DSB) repair in eukaryotic cells. DSB, in principle, can be removed by homologous recombination (HR) and non-homologous end joining (NHEJ). NHEJ apparatus consist of DNA-PK, Ku, XRCC4, DNA ligase IV and other unidentified factors. Our studies indicate that DSB is also repaired by an alternative NHEJ pathway, which is independent of DNA-PK, named B-NHEJ.

Another set of studies is focused on the interaction of checkpoints and DNA repair. Our data show that caffeine sensitizes cells to DNA damage by inhibiting homologous recombination, in which ATR and ATM are involved.

Finally, studies are in progress which center on the mechanism of the maintenance of chromosome integrity in normal and disease stages of the central nervous system (CNS). We investigate DNA repair, DNA replication and checkpoint activation in neural cells after treatment with DNA damaging agents, viral infection, or during aging.

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Wu W., Wang H., and Ma X. 1992. Cloning and expression of a segment of HIV-1 gag gene in E. Coli. Chinese Journal of Microbiology and Immunology, 12:5-8, 1992.

Wang H., and Ma X. Study on expression and processing HAV in insect cells. Chinese Journal of Epidemiology 13:427-430.

Wang, H., Xing R., and Xia S. 1994. Analysis of HPRT mutations induced by ionizing radiation in Hela cells. Chinese Radiation Medicine (Chinese) 8:268.

Wang H., Xing, R., and Liu, F. 1994. Expression of the fusion protein EGF/GM-CSF in E. coli. Chinese Biochemistry and Biophysics (Chinese) 14:42-46.

Wang H., Xing, R., and Xia, S. 1996. Molecular analysis of gamma-ray induce mutation at hprt locus in normal human peripheral lymphocytes. Chinese Biochemical Journal (Chinese), 12:131-137.

Wang H., Shao N., Ding Q.M., Cui J., Reddy E.S., and Rao V.N. 1997. BRCA1 proteins are transported to the nucleus in the absence of serum and splice variants BRCA1a, BRCA1b are tyrosine phosphoproteins that associate with E2F, cyclins and cyclin dependent kinases. Oncogene 15:143-157.

Cui J.Q., Wang H., Reddy E.S., and Rao V.N. 1998. Differential transcriptional activation by the N-terminal region of BRCA1 splice variants BRCA1a and BRCA1b. Oncology Reports 585-589.

Cui J.Q., Shao N., Chai Y., Wang H., Reddy E.S., and Rao V.N. 1998. BRCA1 splice variants BRCA1a and BRCA1b associate with CBP co-activator. Oncology Reports 5:591-595.

Cheong N., Perrault A.R., Wang H., Wachsberger P., Mammen P., Jackson I., and Iliakis G. 1999. DNA-PK-independent rejoining of DNA double-strand breaks in human cell extracts in vitro. Intl. J. Rad. Biol. 75:67-81.

Wang H., Guan J., Wang H., Perrault A.R., Wang Y., and Iliakis G. 2001. Replication Protein A2 Phosphorylation after DNA damage by the coordinated action of ataxia telangiectasia-mutated and DNA-dependent protein kinase. Cancer Res. 61:8554-63.

Perrault R., Cheong N., Wang H., Wang H., and Iliakis G. 2001. RPA facilitates rejoining of DNA double-strand reaks in an in vitro assay utilizing genomic DNA as substrate. Intl. J. Rad. Bio. 77:593-607.

Wang H., Zeng Z.C., Bui T.A., DiBiase S.J., Qin W., Xia F., Powell S.N., and Iliakis G. 2001. Non-homologous end-joining of ionizing radiation-induced DNA double stranded breaks in human tumor cells deficient in BRCA1 or BRCA2. Cancer Res. 61:270-277.

Wang H., Zeng Z.C., Bui T.A., Sonoda E., Takata M., Takeda S., and Iliakis G. 2001. Efficient rejoining of radiation-induced DNA double-strand breaks in vertebrate cells deficient in genes of the RAD52 epistasis group. Oncogene 20:2212-2224.

Wang H., Zeng Z.C., Perrault A.R., Cheng X., Qin W., and Iliakis G. 2001. Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joing in mammalian cells. Nucl. Acids Res. 29:1653-1660.

Wang X., Wang H., Iliakis G., and Wang Y. 2003. Caffeine-induced radiosensitization is independent of nonhomologous end joing of DNA double-strand breaks. Radiat Res. 159:426-432.

Sharma, G. G., Gupta A., Wang H., Scherthan H., Dhar S., Gandhi V., Iliakis G., Shay J.W., Young C.S., and Pandita T.K. 2003. hTERT associates with human telomeres and enhances genomic stability and DNA repair. Oncogene 22:131-146.

Iliakis G., Wang Y., Guan J., and Wang H. 2003. DNA damage checkpoint control in cells exposed to ionizing radiation. Oncogene 22:5834-5847.

Wang H., Perrault A.R., Takeda Y., Qin W., Wang H., and Iliakis G. 2003. Biochemical evidence for Ku-independent backup pathways of NHEJ. Nucl. Acids Res. 31:5377-5388.

Wang H., Wang X., Iliakis G., and Wang Y. 2003. Caffeine could not efficiently sensitize homologous recombination repair-deficient cells to ionizing radiation-induced killing. Radiat. Res. 159:420-425.

Wang H., Wang M., Wang H., Bocker W., and Iliakis G. 2005. Complex H2AX phosphorylation patterns by multiple kinases including ATM and DNA-PK in human cells exposed to ionizing radiation and treated with kinase inhibitors. J Cell Physiol.

Perrault R., Wang H., Wang M., Rosidi B., and Iliakis G. 2004. Backup pathways of NHEJ are suppressed by DNA-PK. J. Cell. Biochem. 92:781-794.

Iliakis G., Wang H., Perrault A. R., Boecker W., Rosidi B., Windhofer F., Wu W., Guan J., Terzoudi G., and Pantelias G. 2004. Mechanisms of DNA double strand break repair and chromosome aberration formation. Cytogenet. Genome Res. 104: 14-20.

Wang H., Wang H., Powell S.N., Iliakis G., and Wang Y. 2004. ATR affecting cell radiosensitivity is dependent on homologous recombination repair but independent of nonhomologous end joining. Cancer Res. 64:7139-7143.

Wang H., Boecker W., Wang H., Wang X., Guan J., Thompson L.H., Nickoloff J.A., and Iliakis G. 2004. Caffeine inhibits homology-directed repair of I-SceI-induced DNA double-strand breaks. Oncogene 23:824-834.

Wang H., Rosidi B., Perrault R., Wang M., Zhang L., Windhofer F. and Iliakis H. 2005. DNA ligase III as a candidate component of backup pathways of NHEJ. Cancer Res. 65:4020-4030.

Iliakis G., Rosidi B., Wang M., and Wang H. 2005. Plasmid-based assays for DNA end joining in vitro. DNA repair Protocol, Mammalian Systems, Second Edition. Henderson Daryl S. (State University of New York at Stony Brook, NY). Methods in Molecular Biology, Volume 314, ISBN 1-59259-973-7.

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