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Ilker K. Sariyer, DVM, PhD

Ilker K. Sariyer, DVM, PhD


Assistant Professor, Neuroscience

Assistant Professor, Neurovirology

Location: Room 753 MERB

Telephone:  215-707-6337

Fax:  215-707-4888

Email: ilker.sariyer0001@temple.edu


Department of Neuroscience

Center for Neurovirology


Educational Background:


DVM, Selcuk University, School of Veterinary Medicine, Konya,
Turkey, 2000


PhD, Biomedical Sciences, Temple University School of Medicine, Philadelphia, PA, 2011


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Research Interests:


My Research interests lie in understanding the molecular mechanism of JC virus reactivation in immunocompromised individuals, identifying novel biomarkers for the early detection of the viral reactivation, and developing therapeutic interventions for the treatment of progressive multifocal leucoencephalopathy (PML), a fetal demyelinating disease of the brain caused by JC virus.


Research Summary:


Patients undergoing immune modulatory therapies for the treatment of autoimmune diseases such as multiple sclerosis, and individuals with an impaired-immune system, most notably AIDS patients, are in the high risk group of developing progressive multifocal leukoencephalopath (PML), a fatal demyelinating disease of the white matter caused by human neurotropic polyomavirus, JC virus. We employ multidisciplinary strategies to determine molecular mechanism of JC virus reactivation during the latent period of viral infection. JC virus replicates almost exclusively in glial cells, and its promoter sequence, which has tissue-specific characteristics, tightly modulates expression of viral genome in appropriate cell types and immunoconditions through communication with cellular factors. We identified the alternative splicing factor, SF2/ASF, as a potential regulator of JCV as its overexpression in glial cells strongly suppresses viral gene expression and replication. Our studies have demonstrated that SF2/ASF expression in glial cells is tightly controlled by immune mediators secreted by PBMCs suggesting a novel neuroimmune signaling between immune cells and glial cells that controls the gene expression and replication of the virus. In addition, a major effort in our laboratory is focused on biomarker potential of agnoprotein, a small regulatory protein of JC virus, for the early diagnosis and the prognosis of the PML.


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Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

26596376. Craigie M, Regan P, Otalora YL, Sariyer IK, Molecular interplay between T-Antigen and splicing factor, arginine/serine-rich 1 (SRSF1) controls JC virus gene expression in glial cells. Virol J 12:1(196)2015 Nov 24

26553431. Regan PM, Sariyer IK, Langford TD, Datta PK, Khalili K, Morphine-Induced MOR-1X and ASF/SF2 Expression Is Independent of Transcriptional Regulation: Implications for MOR-1X Signaling. J Cell Physiol :()2015 Nov 10

26176554. Saribas AS, Khalili K, Sariyer IK, Dysregulation of autophagy by HIV-1 Nef in human astrocytes. Cell Cycle 14:18(2899-904)2015 Sep 17

26061652. De-Simone FI, Sariyer R, Otalora YL, Yarandi S, Craigie M, Gordon J, Sariyer IK, IFN-Gamma Inhibits JC Virus Replication in Glial Cells by Suppressing T-Antigen Expression. PLoS One 10:6(e0129694)2015

25483098. Bruno AP, De Simone FI, Iorio V, De Marco M, Khalili K, Sariyer IK, Capunzo M, Nori SL, Rosati A, HIV-1 Tat protein induces glial cell autophagy through enhancement of BAG3 protein levels. Cell Cycle 13:23(3640-4)2014

25204229. Merabova N, Sariyer IK, Saribas AS, Knezevic T, Gordon J, Turco MC, Rosati A, Weaver M, Landry J, Khalili K, WW domain of BAG3 is required for the induction of autophagy in glioma cells. J Cell Physiol 230:4(831-41)2015 Apr

25151063. Otlu O, De Simone FI, Otalora YL, Khalili K, Sariyer IK, The agnoprotein of polyomavirus JC is released by infected cells: evidence for its cellular uptake by uninfected neighboring cells. Virology 468-470:(88-95)2014 Nov

23975826. Sariyer IK, Transfection of neuronal cultures. Methods Mol Biol 1078:(133-9)2013

23805194. Gordon J, Sariyer IK, De La Fuente-Granada M, Augelli BJ, Otte J, Azizi SA, Amini S, Khalili K, Krynska B, Neural Crest Cells Isolated from the Bone Marrow of Transgenic Mice Express JCV T-Antigen. PLoS One 8:6(e65947)2013

23747198. Sami Saribas A, Abou-Gharbia M, Childers W, Sariyer IK, White MK, Safak M, Essential roles of Leu/Ile/Phe-rich domain of JC virus agnoprotein in dimer/oligomer formation, protein stability and splicing of viral transcripts. Virology 443:1(161-76)2013 Aug 15

23672192. Uleri E, Regan P, Dolei A, Sariyer IK, SF2/ASF binding region within JC virus NCCR limits early gene transcription in glial cells. Virol J 10:(147)2013 May 14

23308224. Beltrami S, Branchetti E, Sariyer IK, Otte J, Weaver M, Gordon J, Neurofibromatosis type 2 tumor suppressor protein, NF2, induces proteasome-mediated degradation of JC virus T-antigen in human glioblastoma. PLoS One 8:1(e53447)2013

22984599. Sariyer IK, Merabova N, Patel PK, Knezevic T, Rosati A, Turco MC, Khalili K, Bag3-induced autophagy is associated with degradation of JCV oncoprotein, T-Ag. PLoS One 7:9(e45000)2012

22496891. Noch E, Sariyer IK, Gordon J, Khalili K, JC virus T-antigen regulates glucose metabolic pathways in brain tumor cells. PLoS One 7:4(e35054)2012

22207898. Uleri E, Beltrami S, Gordon J, Dolei A, Sariyer IK, Extinction of Tumor Antigen Expression by SF2/ASF in JCV-Transformed Cells. Genes Cancer 2:7(728-36)2011 Jul

21609431. Sariyer IK, Saribas AS, White MK, Safak M, Infection by agnoprotein-negative mutants of polyomavirus JC and SV40 results in the release of virions that are mostly deficient in DNA content. Virol J 8:(255)2011 May 24

21297941. Sariyer IK, Khalili K, Regulation of human neurotropic JC virus replication by alternative splicing factor SF2/ASF in glial cells. PLoS One 6:1(e14630)2011 Jan 31

19442856. Sariyer IK, Safak M, Gordon J, Khalili K, Generation and characterization of JCV permissive hybrid cell lines. J Virol Methods 159:1(122-6)2009 Jul

18353421. Romagnoli L, Sariyer IK, Tung J, Feliciano M, Sawaya BE, Del Valle L, Ferrante P, Khalili K, Safak M, White MK, Early growth response-1 protein is induced by JC virus infection and binds and regulates the JC virus promoter. Virology 375:2(331-41)2008 Jun 5

18353419. Sariyer IK, Khalili K, Safak M, Dephosphorylation of JC virus agnoprotein by protein phosphatase 2A: inhibition by small t antigen. Virology 375:2(464-79)2008 Jun 5

18230652. Staniszewska I, Sariyer IK, Lecht S, Brown MC, Walsh EM, Tuszynski GP, Safak M, Lazarovici P, Marcinkiewicz C, Integrin alpha9 beta1 is a receptor for nerve growth factor and other neurotrophins. J Cell Sci 121:Pt 4(504-13)2008 Feb 15

18022798. Khalili K, Sariyer IK, Safak M, Small tumor antigen of polyomaviruses: role in viral life cycle and cell transformation. J Cell Physiol 215:2(309-19)2008 May

16571806. Sariyer IK, Akan I, Palermo V, Gordon J, Khalili K, Safak M, Phosphorylation mutants of JC virus agnoprotein are unable to sustain the viral infection cycle. J Virol 80:8(3893-903)2006 Apr

16497349. Akan I, Sariyer IK, Biffi R, Palermo V, Woolridge S, White MK, Amini S, Khalili K, Safak M, Human polyomavirus JCV late leader peptide region contains important regulatory elements. Virology 349:1(66-78)2006 May 25

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