Figure: Immunolocalization of OA in bone.
Immunohistochemical (A-C) and immunofluorescent (D) localization of OA in proximal tibial metaphysis of 2 week-old normal rats. In A-C, the reaction product has a brown color and all sections were lightly counterstained with hematoxylin. (A) Section representing a control in which the primary antibody was pre-adsorbed with the immunizing peptide; note the absence of any reaction product. (B-D) Medium and high power photomicrographs of sections incubated with anti-OA antibody (Ab-551). Note the reaction product (B and C) or immunofluorescent staining (D) is predominantly associated with rows of osteoblasts (arrows) lining the bone (b). p = periosteum; m = marrow spaces. Magnifications: A & B = X170; C = X400; D = X280.