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Steven Popoff


Steven N. Popoff , PhD


John Franklin Huber Chair

Professor, Anatomy and Cell Biology

Telephone:  215-707- 3163

Fax:  215-707-2966

Email: spopoff@temple.edu


Department of Anatomy and Cell Biology


Educational Background:


Loyola University, Chicago, IL

B.S., Biology


Loyola University, Stritch School of Medicine, Maywood, IL

Ph.D., Cell Biology/Anatomy (Advisor:  Gary B. Schneider)


University of Massachusetts Medical School, Worcester, MA

Postdoctoral Fellowship (Sponsor: Sandy C. Marks, Jr.)


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Research Interests:


Steven N. Popoff, Ph.D., Professor and Chairperson of the Department of Anatomy and Cell Biology, is a bone cell biologist with training and experience in cellular and molecular approaches as they are applied to the study of mineralized tissues. His research focuses on the regulation of skeletal development and the pathogenesis of various metabolic bone diseases including congenital osteopetrosis, inflammation-mediated osteopenia (i.e., rheumatoid arthritis, osteoarthritis and periodontal disease) and estrogen-deficient osteoporosis. This area of research is important because bone diseases are a major cause of mortality and morbidity in the United States and throughout the world. The need for increased research efforts in bone disorders has been designated as a high priority by many health-care and research organizations including the National Institutes of Health. The incidence of osteoporosis, with the estrogen-deficient post-menopausal form being the most prevalent, has increased dramatically as life expectancy continues to increase. The various forms of osteoporosis have been implicated in more than 1.5 million fractures per year in the United States alone, with estimated health care costs of $7 to $10 billion annually. Bone loss (osteopenia) is also a common occurrence in association with chronic inflammatory disease states, such as rheumatoid arthritis, osteoarthritis and periodontal disease. Diseases affecting skeletal development, such as osteogenesis imperfecta and osteopetrosis, account for a significant portion of birth defects and childhood disorders. Dr. Popoff’s laboratory utilizes various animal models of congenital or induced bone diseases that influence the development or maintenance of skeletal tissues. Recently, his laboratory identified a novel regulator of osteoclast function that is believed to play an important role in mediating the bone loss associated with chronic inflammatory conditions. Dr. Popoff’s lab is also working on cloning and functional characterization of two novel genes, connective tissue growth factor and osteoactivin, that appear to play a important roles in osteogenesis during skeletal development, skeletal remodeling and fracture repair. These studies will provide insights regarding the precise and coordinated regulation of bone formation and resorption. Ultimately, this information will lead to the development of novel therapeutic strategies to either block excessive bone resorption or stimulate bone formation in specific bone diseases where these effects would produce a desirable clinical outcome, namely the preservation of bone mass and strength.


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Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

26245773. Ali S, Cunningham R, Amin M, Popoff SN, Mohamed F, Barbe MF, The extensor carpi ulnaris pseudolesion: evaluation with microCT, histology, and MRI. Skeletal Radiol :()2015 Aug 6

25899717. Frara N, Abdelmagid SM, Sondag GR, Moussa FM, Yingling VR, Owen TA, Popoff SN, Barbe MF, Safadi FF, Transgenic Expression of Osteoactivin/gpnmb Enhances Bone Formation In Vivo and Osteoprogenitor Differentiation Ex Vivo. J Cell Physiol 231:1(72-83)2016 Jan

25739896. Barbe MF, Jain NX, Massicotte VS, Popoff SN, Barr-Gillespie AE, Ergonomic task reduction prevents bone osteopenia in a rat model of upper extremity overuse. Ind Health 53:3(206-21)2015

25714841. Hendesi H, Barbe MF, Safadi FF, Monroy MA, Popoff SN, Integrin mediated adhesion of osteoblasts to connective tissue growth factor (CTGF/CCN2) induces cytoskeleton reorganization and cell differentiation. PLoS One 10:2(e0115325)2015

25617052. Fisher PW, Zhao Y, Rico MC, Massicotte VS, Wade CK, Litvin J, Bove GM, Popoff SN, Barbe MF, Increased CCN2, substance P and tissue fibrosis are associated with sensorimotor declines in a rat model of repetitive overuse injury. J Cell Commun Signal 9:1(37-54)2015 Mar

24583543. Jain NX, Barr-Gillespie AE, Clark BD, Kietrys DM, Wade CK, Litvin J, Popoff SN, Barbe MF, Bone loss from high repetitive high force loading is prevented by ibuprofen treatment. J Musculoskelet Neuronal Interact 14:1(78-94)2014 Mar

24156755. Barbe MF, Gallagher S, Massicotte VS, Tytell M, Popoff SN, Barr-Gillespie AE, The interaction of force and repetition on musculoskeletal and neural tissue responses and sensorimotor behavior in a rat model of work-related musculoskeletal disorders. BMC Musculoskelet Disord 14:(303)2013 Oct 25

24127409. Mundy C, Gannon M, Popoff SN, Connective tissue growth factor (CTGF/CCN2) negatively regulates BMP-2 induced osteoblast differentiation and signaling. J Cell Physiol 229:5(672-81)2014 May

24015193. Gao HG, Fisher PW, Lambi AG, Wade CK, Barr-Gillespie AE, Popoff SN, Barbe MF, Increased serum and musculotendinous fibrogenic proteins following persistent low-grade inflammation in a rat model of long-term upper extremity overuse. PLoS One 8:8(e71875)2013

23073844. Lambi AG, Pankratz TL, Mundy C, Gannon M, Barbe MF, Richtsmeier JT, Popoff SN, The skeletal site-specific role of connective tissue growth factor in prenatal osteogenesis. Dev Dyn 241:12(1944-59)2012 Dec

22454039. Sharir H, Console-Bram L, Mundy C, Popoff SN, Kapur A, Abood ME, The endocannabinoids anandamide and virodhamine modulate the activity of the candidate cannabinoid receptor GPR55. J Neuroimmune Pharmacol 7:4(856-65)2012 Dec

21807131. Joshi RN, Safadi FF, Barbe MF, Del Carpio-Cano F, Popoff SN, Yingling VR, Different effects on bone strength and cell differentiation in pre pubertal caloric restriction versus hypothalamic suppression. Bone 49:4(810-8)2011 Oct

21503878. Singh M, Del Carpio-Cano FE, Monroy MA, Popoff SN, Safadi FF, Homeodomain transcription factors regulate BMP-2-induced osteoactivin transcription in osteoblasts. J Cell Physiol 227:1(390-9)2012 Jan

20506259. Abdelmagid SM, Barbe MF, Hadjiargyrou M, Owen TA, Razmpour R, Rehman S, Popoff SN, Safadi FF, Temporal and spatial expression of osteoactivin during fracture repair. J Cell Biochem 111:2(295-309)2010 Oct 1

20432467. Zhang X, Arnott JA, Rehman S, Delong WG Jr, Sanjay A, Safadi FF, Popoff SN, Src is a major signaling component for CTGF induction by TGF-beta1 in osteoblasts. J Cell Physiol 224:3(691-701)2010 Sep

21395506. Singh M, Del Carpio-Cano F, Belcher JY, Crawford K, Frara N, Owen TA, Popoff SN, Safadi FF, Functional roles of osteoactivin in normal and disease processes. Crit Rev Eukaryot Gene Expr 20:4(341-57)2010

19006175. Zhu S, Barbe MF, Liu C, Hadjiargyrou M, Popoff SN, Rani S, Safadi FF, Litvin J, Periostin-like-factor in osteogenesis. J Cell Physiol 218:3(584-92)2009 Mar

18555216. Abdelmagid SM, Barbe MF, Rico MC, Salihoglu S, Arango-Hisijara I, Selim AH, Anderson MG, Owen TA, Popoff SN, Safadi FF, Osteoactivin, an anabolic factor that regulates osteoblast differentiation and function. Exp Cell Res 314:13(2334-51)2008 Aug 1

18464247. Barbe MF, Elliott MB, Abdelmagid SM, Amin M, Popoff SN, Safadi FF, Barr AE, Serum and tissue cytokines and chemokines increase with repetitive upper extremity tasks. J Orthop Res 26:10(1320-6)2008 Oct

18314002. Arnott JA, Zhang X, Sanjay A, Owen TA, Smock SL, Rehman S, DeLong WG, Safadi FF, Popoff SN, Molecular requirements for induction of CTGF expression by TGF-beta1 in primary osteoblasts. Bone 42:5(871-85)2008 May

17219411. Rico MC, Castaneda JL, Manns JM, Uknis AB, Sainz IM, Safadi FF, Popoff SN, Dela Cadena RA, Amelioration of inflammation, angiogenesis and CTGF expression in an arthritis model by a TSP1-derived peptide treatment. J Cell Physiol 211:2(504-12)2007 May

17133352. Arnott JA, Nuglozeh E, Rico MC, Arango-Hisijara I, Odgren PR, Safadi FF, Popoff SN, Connective tissue growth factor (CTGF/CCN2) is a downstream mediator for TGF-beta1-induced extracellular matrix production in osteoblasts. J Cell Physiol 210:3(843-52)2007 Mar

17111364. Song JJ, Aswad R, Kanaan RA, Rico MC, Owen TA, Barbe MF, Safadi FF, Popoff SN, Connective tissue growth factor (CTGF) acts as a downstream mediator of TGF-beta1 to induce mesenchymal cell condensation. J Cell Physiol 210:2(398-410)2007 Feb

17034042. Abdelmagid SM, Barbe MF, Arango-Hisijara I, Owen TA, Popoff SN, Safadi FF, Osteoactivin acts as downstream mediator of BMP-2 effects on osteoblast function. J Cell Physiol 210:1(26-37)2007 Jan

16869004. Manns JM, Uknis AB, Rico MC, Agelan A, Castaneda J, Arango I, Barbe MF, Safadi FF, Popoff SN, DeLa Cadena RA, A peptide from thrombospondin 1 modulates experimental erosive arthritis by regulating connective tissue growth factor. Arthritis Rheum 54:8(2415-22)2006 Aug

14696973. Selim AA, Abdelmagid SM, Kanaan RA, Smock SL, Owen TA, Popoff SN, Safadi FF, Anti-osteoactivin antibody inhibits osteoblast differentiation and function in vitro. Crit Rev Eukaryot Gene Expr 13:2-4(265-75)2003

14606516. Barr AE, Safadi FF, Gorzelany I, Amin M, Popoff SN, Barbe MF, Repetitive, negligible force reaching in rats induces pathological overloading of upper extremity bones. J Bone Miner Res 18:11(2023-32)2003 Nov

12767040. Safadi FF, Xu J, Smock SL, Kanaan RA, Selim AH, Odgren PR, Marks SC Jr, Owen TA, Popoff SN, Expression of connective tissue growth factor in bone: its role in osteoblast proliferation and differentiation in vitro and bone formation in vivo. J Cell Physiol 196:1(51-62)2003 Jul

11746512. Safadi FF, Xu J, Smock SL, Rico MC, Owen TA, Popoff SN, Cloning and characterization of osteoactivin, a novel cDNA expressed in osteoblasts. J Cell Biochem 84:1(12-26)2001

10855695. Safadi FF, Dissanayake IR, Goodman GG, Jago RA, Baker AE, Bowman AR, Sass DA, Popoff SN, Epstein S, Influence of estrogen deficiency and replacement on T-cell populations in rat lymphoid tissues and organs. Endocrine 12:1(81-8)2000 Feb

10786828. Safadi FF, Hermey DC, Popoff SN, Seifert MF, Skeletal resistance to 1,25-dihydroxyvitamin D3 in osteopetrotic rats. Endocrine 11:3(309-19)1999 Dec

10679821. Xu J, Smock SL, Safadi FF, Rosenzweig AB, Odgren PR, Marks SC Jr, Owen TA, Popoff SN, Cloning the full-length cDNA for rat connective tissue growth factor: implications for skeletal development. J Cell Biochem 77:1(103-15)2000 Feb

8796921. Popoff SN, Schneider GB, Animal models of osteopetrosis: the impact of recent molecular developments on novel strategies for therapeutic intervention. Mol Med Today 2:8(349-58)1996 Aug

8579954. Popoff SN, Marks SC Jr, The heterogeneity of the osteopetroses reflects the diversity of cellular influences during skeletal development. Bone 17:5(437-45)1995 Nov

7664677. Hermey DC, Popoff SN, Expression of the vitamin D receptor in the intestine and kidney of osteopetrotic rats. Endocrinology 136:10(4558-64)1995 Oct

7864108. Hermey DC, Ireland RA, Zerwekh JE, Popoff SN, Regulation of mineral homeostasis in osteopetrotic (op) rats. Am J Physiol 268:2 Pt 1(E312-7)1995 Feb

7980962. Popoff SN, Osier LK, Zerwekh JE, Marks SC Jr, Interdependence of skeletal sclerosis and elevated circulating levels of 1,25-dihydroxyvitamin D in osteopetrotic (op and tl) rats. Bone 15:5(515-22)1994 Sep-Oct

8176226. Yamamoto N, Lindsay DD, Naraparaju VR, Ireland RA, Popoff SN, A defect in the inflammation-primed macrophage-activation cascade in osteopetrotic rats. J Immunol 152:10(5100-7)1994 May 15

1759690. Popoff SN, Marks SC Jr, Congenitally osteosclerotic (os/os) rabbits are not cured by bone marrow transplantation from normal littermates. Am J Anat 192:3(274-80)1991 Nov

2035353. Popoff SN, Jackson ME, Koevary SB, Marks SC Jr, Coexistence of reduced function of natural killer cells and osteoclasts in two distinct osteopetrotic mutations in the rat. J Bone Miner Res 6:3(263-71)1991 Mar

2313606. Popoff SN, Marks SC Jr, Relationship of abnormalities in dental and skeletal development in the osteopetrotic (os) rabbit. J Oral Pathol Med 19:1(5-12)1990 Jan

2557510. Popoff SN, Coderre JA, Marks SC Jr, Stock JL, Calcitonin inhibits accumulation of cyclic AMP in stimulated peritoneal macrophages from normal rats but not from osteopetrotic (incisors-absent) littermates. Life Sci 45:21(2007-13)1989

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Loyola University, Stritch School of Medicine

Gross Anatomy, 1982

Histology/Cell Biology, 1983

Embryology, 1983

Neuroscience, 1982-1985

University of Massachusetts Medical School

Gross Anatomy, 1985-1987

Temple University School of Medicine

Gross Anatomy, 1988 - present

Embryology, 1993 - 1997

Cell Biology, 1994 - 1997

Histology, 1998 - 2000, 2003


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Postdoctoral and Other Scientists Supervised

Fayez F. Safadi, PhD, Assistant Scientist, 1998-1999

Current Position – Professor of Anatomy and Neurobiology, Northeast Ohio Medical University


Hwyda Arafat, MD, PhD, Postdoctoral Fellow, 1998-1999

Current Position – Associate Professor of Surgery, Thomas Jefferson University


Marina D’Angelo, PhD, Associate Scientist, 2000-2001

Current Position – Assistant Professor of Anatomy, Philadelphia College of Osteopathic Medicine


Mario C. Rico, MD, Postdoctoral Fellow, 2001-2005

Current Position – Research Assistant Professor, Temple University School of Medicine


Xue-Qing Wang, PhD, Associate Scientist, 2002

Current Position – Research Associate, Fox Chase Cancer Center


Roula Aswad, DDS, Postdoctoral Fellow, 2002-2005

Current Position, Private Practice in Periodontology


Edem Nuglozeh, PhD, Associate Scientist, 2002-2005


Israel Arango, MD, Postdoctoral Fellow, 2003-2009


John A. Arnott, Postdoctoral Fellow, 2006-2008

Current Position – Assistant Professor of Cell Biology, The Commonwealth Medical College


Fabiola del Carpio-Cana, PhD, Postdoctoral Fellow, 2006-2011

Current Position – Associate Scientist, Temple University School of Medicine


Robin A. Pixley, PhD, Research Scientist, 2009-present


Graduate Students Supervised (as primary Advisor)

Donna C. Hermey (PhD Program), 1990-1994

Current Position – Professor of Anatomy, DeBusk College of Osteopathic Medicine


Jie Xu (PhD Program), 1995-2000


Julian Cataneda (MS Program), 2003-2005

Current Position – Resident, Department of Anesthesiology, Temple University School of Medicine


John A. Arnott (PhD Program), 2002-2006

Current Position – Assistant Professor of Cell Biology, The Commonwealth Medical College


Jason J. Song (MD/PhD Program), 2003-2006

Current Position – Resident, Department of Physical Medicine and Rehabilitation, University of Pennsylvania Health System


Xuemei Zhang (PhD Program), 2007-2009

Current Position – Postdoctoral Fellow, University of Pennsylvania


Honey Hendesi (PhD Program), 2009-present


Alex Lambi (MD/PhD program), 2010-present


Christina M. Mundy (PhD Program), 2010-present


Graduate Students Supervised (as co-Advisor)

Reem A. Kanaan (PhD Program), 2001-2003

Current Position – Assistant Professor, Faculty of Pharmacy, Al-Faytoonah University of Jordan


Abdul-Hafez Selim (PhD Program), 2001-2003

Current Position – Assistant Professor of Physics, King Fahd University of Petroleum and Minerals, Saudi Arabia


Samir Abdel-Magid (PhD Program), 2002-2005

Current Position – Research Scientist, Northeast Ohio Medical University


Maneet Singh (PhD Program), 2008-2011


Joyce Belcher (PhD Program), 2006-present


Nagat Frara (PhD Program), 2009-present


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