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Stephen PilderStephen Pilder, PhD

 

Associate Professor, Anatomy and Cell Biology

Assistant Professor, Fels Institute for Cancer Research and Molecular Biology

Telephone:  215-707-8996

Fax:  215-707- 2966

Email: stephen.pilder@temple.edu

 

Department of Anatomy and Cell Biology

Fels Institute for Cancer Research and Molecular Biology

 

Educational Background:

 

Lawrence University, Appleton, WI
Philosophy, 1965-68

 

University of Cincinnati
Cincinnati, OH

Philosophy, 1969-70

B.A, 1970


University of Cincinnati
Cincinnati, OH

Biology, 1980-81

 

Dept of Molecular Microbiology
State University of New York, Stony Brook, NY
Molecular Biology, 1981-84

 

Dept of Molecular Biology
Princeton University, Princeton, NJ

Molecular Biology, 1984-86
Ph.D, 1986

 

Princeton University, Princeton, NJ

Postdoctoral Fellowship,  Molecular Biology of the t Complex-SpecificTcp10 Multigene Family in Mice and Humans, 1986-89

(Sponsor: Dr. Dr. Lee M. Silver)

 

Princeton University, Princeton, NJ

Postdoctoral Fellowship, Molecular Genetics of t Complex-Specific Hybrid Sterility, 1989-91

(Sponsor: Dr. Lee M. Silver)

 

Hemoglobin Department, Gene Expression Division, DNX Corporation, Princeton, NJ

Production of Functional Human Hemoglobin in Transgenic Swine, 1991-92

 

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Research Interests:

 

Stephen H. Pilder, Ph.D., Associate Professor in the Department of Anatomy and Cell Biology, is a reproductive biologist with training and experience in cellular, genetic, and molecular approaches to the study of mammalian spermiogenesis, sperm motility, and sperm-egg interactions. His research focuses on the molecular and physiological bases of mammalian sperm function in the fertilization process, with primary emphasis on mammalian sperm motility. Infertility is a major cause of social, psychological, and economic stress throughout the developed world. Because increased research efforts into the genetic and molecular bases of male infertility hold promise for the development of advanced diagnostic tools and effective, less physically intrusive, and less expensive assisted reproductive therapies, this research is strongly supported by the National Institutes of Health. Dr. Pilder’s laboratory has engineered and presently utilizes numerous mouse models of male infertility that provide the material for isolating and studying the networks of genes responsible for numerous male infertility phenotypes. Recently, he and members of his laboratory have identified and isolated a tightly-linked complex of genes that operate in several signaling pathways to control unrelated calcium-dependent aspects of sperm function, such as hyperactivated motility, zona pellucida binding, and penetration of the egg plasma membrane. Dr. Pilder’s lab is currently characterizing these genes and the pathways in which they act. These studies are expected to provide insights into the coordinated regulation of mammalian sperm function and the basic molecular mechanisms underlying male fertility.

 

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PUBMED PUBLICATIONS :


Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

17566267. Pilder SH, Lu J, Han Y, Hui L, Samant SA, Olugbemiga OO, Meyers KW, Cheng L, Vijayaraghavan S, The molecular basis of "curlicue": a sperm motility abnormality linked to the sterility of t haplotype homozygous male mice. Soc Reprod Fertil Suppl 63:(123-33)2007

16354795. Hui L, Lu J, Han Y, Pilder SH, The mouse T complex gene Tsga2, encoding polypeptides located in the sperm tail and anterior acrosome, maps to a locus associated with sperm motility and sperm-egg interaction abnormalities. Biol Reprod 74:4(633-43)2006 Apr

16054618. Samant SA, Ogunkua OO, Hui L, Lu J, Han Y, Orth JM, Pilder SH, The mouse t complex distorter/sterility candidate, Dnahc8, expresses a gamma-type axonemal dynein heavy chain isoform confined to the principal piece of the sperm tail. Dev Biol 285:1(57-69)2005 Sep 1

12297094. Samant SA, Ogunkua O, Hui L, Fossella J, Pilder SH, The T complex distorter 2 candidate gene, Dnahc8, encodes at least two testis-specific axonemal dynein heavy chains that differ extensively at their amino and carboxyl termini. Dev Biol 250:1(24-43)2002 Oct 1

11023686. Redkar AA, Si Y, Twine SN, Pilder SH, Olds-Clarke P, Genes in the first and fourth inversions of the mouse t complex synergistically mediate sperm capacitation and interactions with the oocyte. Dev Biol 226:2(267-80)2000 Oct 15

9922385. Samant SA, Fossella J, Silver LM, Pilder SH, Mapping and cloning recombinant breakpoints demarcating the hybrid sterility 6-specific sperm tail assembly defect. Mamm Genome 10:2(88-94)1999 Feb

9745038. Redkar AA, Olds-Clarke P, Dugan LM, Pilder SH, High-resolution mapping of sperm function defects in the t complex fourth inversion. Mamm Genome 9:10(825-30)1998 Oct

9432139. Pilder SH, Olds-Clarke P, Orth JM, Jester WF, Dugan L, Hst7: a male sterility mutation perturbing sperm motility, flagellar assembly, and mitochondrial sheath differentiation. J Androl 18:6(663-71)1997 Nov-Dec

9283007. Orth JM, Qiu J, Jester WF Jr, Pilder S, Expression of the c-kit gene is critical for migration of neonatal rat gonocytes in vitro. Biol Reprod 57:3(676-83)1997 Sep

9096116. Pilder SH, Identification and linkage mapping of Hst7, a new M. spretus/M. m. domesticus chromosome 17 hybrid sterility locus. Mamm Genome 8:4(290-1)1997 Apr

8600017. Johnson LR, Pilder SH, Olds-Clarke P, The cellular basis for interaction of sterility factors in the mouse t haplotype. Genet Res 66:3(189-93)1995 Dec

7883069. Johnson LR, Pilder SH, Bailey JL, Olds-Clarke P, Sperm from mice carrying one or two t haplotypes are deficient in investment and oocyte penetration. Dev Biol 168:1(138-49)1995 Mar

8405685. Pilder SH, Olds-Clarke P, Phillips DM, Silver LM, Hybrid sterility-6: a mouse t complex locus controlling sperm flagellar assembly and movement. Dev Biol 159:2(631-42)1993 Oct

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Teaching:

 

Princeton University Mentor

Undergraduate Honors Research Projects
Department of Molecular Biology
1987 - 1990


Temple University School of Medicine

Gross Anatomy, 1993 - present
Cell Biology, 1993 - present
Developmental Biology, 1995 - present
Seminars in Cell Biology, 1999 - present
Course Director 2002 - present


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Trainees:

 

Postdoctoral Fellows and Other Scientists Supervised

Usha Rani Pyapali, Ph.D., 1995-1996

 

Sadhana A. Samant, Ph.D., 1995-2003

 

Anjali Redkar, Ph.D., 1997-2001

 

Yibing Han, Ph.D., 2002-2005

 

Graduate Students Supervised

Mr. Ge Jin: Instructor in lab rotation 1996  

 

Ms.Yelin Yang: Instructor in lab rotation, 1997

 

Ms.Molinda Lausingh: Instructor in lab rotation, 1997

 

Dr. Olugbemiga Ogunkua: Instructor in lab rotation, 1999

 

Ms. Ling, Hui: Instructor in lab rotation, 1999

 

Dr. Olugbemiga Ogunkua: PhD Dissertation Advisor, 2000-2003

(Received PhD, August, 2003)

 

Dr. Ling Hui: PhD Dissertation Advisor, 2000-2004

(Received PhD, May, 2004)

 

Ms. Lauren LeBeau: Master’s Thesis Advisor, 2004-2005

(Received Degree, May, 2005)

 

Ms. Jing Lu, PhD Dissertation Advisor,  2004-present

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