Immunofluorescent localization of TSGA2 with a C-terminal antibody. Left hand panel, negative phase contrast image of cauda epididymal sperm from C57BL/6 mouse. Center panel, DAPI-stained nucleus. Right hand panel, immunofluorecence showing TSGA2 localization to the acrosomal crescent, and the midpiece and proximal principal piece of the sperm flagellum.