Figure 4: Effect of rCTGF on bone formation in vivo.
Photomicrographs of H&E stained sections of the diaphysis from control (A) and rCTGF (B and C) injected femurs. (A) Low power photomicrograph showing normal cortical bone (cb) and marrow (m) in a saline/BSA-injected control femur. There was no evidence of de novo bone formation anywhere in the marrow cavity. (B) Low power photomicrograph of similar region shown in A, but in a rCTGF-injected femur showing a large island of newly formed woven bone (outlined by arrows). (C) Higher power photomicrographs of bone formed in response to rCTGF showing bony trabeculae (t) lined with active osteoblasts. In some areas, especially at the periphery, there are rows of osteoblasts and/or osteoprogenitors piled two or more layers deep (arrows). Note that the marrow (m) immediately adjacent to newly formed bone has an abundance of vascular channels. Magnifications: A & B = X60; C = X130.