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Walter Long, PhD

Professor, Microbiology and Immunology

Professor, Sol Sherry Thrombosis Research Center

Professor, Fels Institute for Cancer Research and Molecular Biology

Telephone: 215-707-4984

Fax: 215-707-7788

Email: longwk@temple.edu

Department of Microbiology and Immunology

Sol Sherry Thrombosis Research Center

Fels Institute for Cancer Research and Molecular Biology

University of Illinois, PhD

 

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1 Intranuclear Immunization. Role: P.I. In collaboration with E.E. Henderson (HIV) and K.J. Soprano (RARα)
The purpose of this project is to develop a mechanism for the regulated expression of single chain antibodies (linked heavy and light chain variable fragments) in cells and the transport of such antibodies to the nucleus of the cell. A system of vectors has been constructed and a monoclonal antibody to HIV integrase has been successfully introduced and expressed blocking the replication of HIV. A library of monoclonal antibodies has been generated to retinoic acid receptor RARα. One of these has been cloned and will be introduced into the vector system to determine its effect on the cellular response to retinoids. Additional antibodies will also be cloned and tested in hopes of mapping retinoid response elements.

2. Angiogenesis. Role: Co-investigator. In collaboration with Dr. R.W. Colman, and Dr. I.M. Sainz
A monoclonal antibody directed against D5 of high molecular weight kininogen has been shown to inhibit neovascularization of the chicken chorioallantoic membrane. When injected into mice the hybridoma producing this antibody gives rise to tumors which are significantly smaller than those produced by control hybridomas. Vascular density in these tumors is also significantly less than controls and the tumors regress by 22 days post injection, as opposed to continued and rapid growth of control hybridomas. The kinetics and mechanisms of these findings are being investigated to determine whether there is potential for clinical application of this antibody.

3. Gene Therapy. Role: Co-investigator. In collaboration with Dr. R. Rolandelli, and Dr. J. Migaly
A role for TGF-β gene therapy in intestinal surgery is being investigated. Maintaining tissue viability following colon resectioning is a common problem. There is evidence that the cytokine TGF-β can improve tissue viability and healing following surgery. Currently the TGF-β gene is expressed in an adenovirus vector. Dr. Long has expertise in virology and molecular biology. He is involved in propagation of the vector, subcloning the TGF-β gene into a more suitable adeno-associated virus vector, and determining levels of TGF-β expression.

4. Generation of Monoclonal Antibodies to Opioid Receptors. Role: Co-investigator. In collaboration with Dr. T. Rogers
Monoclonal antibodies to opioid receptors will be generated. Attempts to generate monoclonal antibodies to opioid receptors by a number of investigators have been unsuccessful because the receptor proteins are recognized as self. To avoid this problem, knockout mice which do not express receptors will be immunized and used as a source of cells in generating hybridomas producing the appropriate antibodies.

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Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

18262563. Gupta D, Palma J, Molina E, Gaughan JP, Long W, Houser S, Macha M, Improved exercise capacity and reduced systemic inflammation after adenoviral-mediated SERCA-2a gene transfer. J Surg Res 145:2(257-65)2008 Apr

17332116. De Long WG Jr, Einhorn TA, Koval K, McKee M, Smith W, Sanders R, Watson T, Bone grafts and bone graft substitutes in orthopaedic trauma surgery. A critical analysis. J Bone Joint Surg Am 89:3(649-58)2007 Mar

16187087. Sainz IM, Isordia-Salas I, Espinola RG, Long WK, Pixley RA, Colman RW, Multiple myeloma in a murine syngeneic model:modulation of growth and angiogenesis by a monoclonal antibody to kininogen. Cancer Immunol Immunother 55:7(797-807)2006 Jul

15540302. Migaly J, Lieberman J, Long W, Fisher C, Rolandelli RH, Effect of adenoviral-mediated transfer of transforming growth factor-beta1 on colonic anastomotic healing. Dis Colon Rectum 47:10(1699-705)2004 Oct

15331420. Espinola RG, Uknis A, Sainz IM, Isordia-Salas I, Pixley R, DeLa Cadena R, Long W, Agelan A, Gaughan J, Adam A, Colman RW, A monoclonal antibody to high-molecular weight kininogen is therapeutic in a rodent model of reactive arthritis. Am J Pathol 165:3(969-76)2004 Sep

9344904. Romano G, Guan M, Long WK, Henderson EE, Differential effects on HIV-1 gene regulation by EBV in T lymphocytic and promonocytic cells transduced to express recombinant human CR2. Virology 237:1(23-32)1997 Oct 13

8212555. Nonkwelo CB, Long WK, Regulation of Epstein-Barr virus BamHI-H divergent promoter by DNA methylation. Virology 197:1(205-15)1993 Nov

1987045. Fratamico PM, Long WK, Buckley HR, Production and characterization of monoclonal antibodies to a 58-kilodalton antigen of Aspergillus fumigatus. Infect Immun 59:1(316-22)1991 Jan

2538121. Fronko GE, Long WK, Wu B, Papadopoulos T, Henderson EE, Relationship between methylation status and expression of an Epstein-Barr virus (EBV) capsid antigen gene. Biochem Biophys Res Commun 159:1(263-70)1989 Feb 28

2446955. Valerie K, Fronko G, Long W, Henderson EE, Nilsson B, Uhlén M, de Riel JK, Production and detection of coliphage T4 endonuclease V polyclonal and monoclonal antibodies using staphylococcal protein-A hybrid proteins. Gene 58:1(99-107)1987

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Director of M212, Microbiology and Immunology Medical Course; Assistant Chairperson for Professional Teaching and Administration.

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Current or Recent Grant Support of Active Projects:

Walter Long , PhD as Co-Investigator:

National Institutes of Health, P30 DA13429, M. W. Adler, PhD, Principal Investigator; Center on Intersystem Regulation by Drugs of Abuse

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