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Lee Liu-chen, PhDLee-Yuan Liu-Chen, PhD


Professor, Pharmacology

Telephone:  215-707-4188

Fax:  215-707-6661

Email: lee-yuan.liu-chen@temple.edu


Department of Pharmacology

Center for Substance Abuse Research


Educational Background:


BS, National Taiwan University, Taipei, Taiwan 1976


MS, University of Minnesota (Mayo Clinic), Rochester, MN 1980


PhD, Massachusetts Institue of Technology, Cambridge, MA 1983


Postdoctoral Fellowship, Massachusetts General Hospital/Harvard Medical School, Boston, MA 1984

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Research Interests:


  • Biochemistry and molecular and cellular pharmacology of opioid receptors.
  • Mechanisms underlying drug abuse.


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Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

26057692. DiMattio KM, Ehlert FJ, Liu-Chen LY, Intrinsic relative activities of ? opioid agonists in activating Ga proteins and internalizing receptor: Differences between human and mouse receptors. Eur J Pharmacol 761:(235-44)2015 Aug 15

25481857. DiMattio KM, Chen C, Shi L, Liu-Chen LY, K3036·58 in the µ opioid (MOP) receptor is important in conferring selectivity for covalent binding of ß-funaltrexamine (ß-FNA). Eur J Pharmacol 748:(93-100)2015 Feb 5

25293322. Huang P, Chen C, Liu-Chen LY, Detection of mu opioid receptor (MOPR) and its glycosylation in rat and mouse brains by western blot with anti-µC, an affinity-purified polyclonal anti-MOPR antibody. Methods Mol Biol 1230:(141-54)2015

25293321. Chen C, Huang P, Liu-Chen LY, Identification and verification of proteins interacting with the kappa opioid receptor (KOPR). Methods Mol Biol 1230:(129-40)2015

24503508. Dimattio KM, Yakovleva TV, Aldrich JV, Cowan A, Liu-Chen LY, Zyklophin, a short-acting kappa opioid antagonist, induces scratching in mice. Neurosci Lett 563:(155-9)2014 Mar 20

24454571. Munro TA, Xu W, Ho DM, Liu-Chen LY, Cohen BM, Studies toward bivalent ? opioids derived from salvinorin A: heteromethylation of the furan ring reduces affinity. Beilstein J Org Chem 9:(2916-24)2013 Dec 20

24080315. Xu W, Wang Y, Ma Z, Chiu YT, Huang P, Rasakham K, Unterwald E, Lee DY, Liu-Chen LY, L-isocorypalmine reduces behavioral sensitization and rewarding effects of cocaine in mice by acting on dopamine receptors. Drug Alcohol Depend 133:2(693-703)2013 Dec 1

23856011. Huang P, Chiu YT, Chen C, Wang Y, Liu-Chen LY, A G protein-coupled receptor (GPCR) in red: live cell imaging of the kappa opioid receptor-tdTomato fusion protein (KOPR-tdT) in neuronal cells. J Pharmacol Toxicol Methods 68:3(340-5)2013 Nov-Dec

23624231. Xu W, Chen C, Li JG, Dimattio K, Wang Y, Unterwald E, Liu-Chen LY, PKA and ERK1/2 are involved in dopamine D1 receptor-induced heterologous desensitization of the d opioid receptor. Life Sci 92:23(1101-9)2013 Jun 21

23446450. Van't Veer A, Bechtholt AJ, Onvani S, Potter D, Wang Y, Liu-Chen LY, Schütz G, Chartoff EH, Rudolph U, Cohen BM, Carlezon WA Jr, Ablation of kappa-opioid receptors from brain dopamine neurons has anxiolytic-like effects and enhances cocaine-induced plasticity. Neuropsychopharmacology 38:8(1585-97)2013 Jul

23086943. Schattauer SS, Miyatake M, Shankar H, Zietz C, Levin JR, Liu-Chen LY, Gurevich VV, Rieder MJ, Chavkin C, Ligand directed signaling differences between rodent and human ?-opioid receptors. J Biol Chem 287:50(41595-607)2012 Dec 7

22989890. Li JG, Chen C, Huang P, Wang Y, Liu-Chen LY, 14-3-3? Protein regulates anterograde transport of the human ?-opioid receptor (hKOPR). J Biol Chem 287:45(37778-92)2012 Nov 2

22863678. Rasakham K, McGillivray KL, Liu-Chen LY, Sex differences in U50,488H-induced phosphorylation of p44/42 mitogen-activated protein kinase in the guinea pig brain. Neuroscience 223:(447-56)2012 Oct 25

22862850. Wang YJ, Huang P, Blendy JA, Liu-Chen LY, Brain region- and sex-specific alterations in DAMGO-stimulated [(35) S]GTP?S binding in mice with Oprm1 A112G. Addict Biol 19:3(354-61)2014 May

22736766. Rives ML, Rossillo M, Liu-Chen LY, Javitch JA, 6'-Guanidinonaltrindole (6'-GNTI) is a G protein-biased ?-opioid receptor agonist that inhibits arrestin recruitment. J Biol Chem 287:32(27050-4)2012 Aug 3

22652295. Lisek R, Xu W, Yuvasheva E, Chiu YT, Reitz AB, Liu-Chen LY, Rawls SM, Mephedrone ('bath salt') elicits conditioned place preference and dopamine-sensitive motor activation. Drug Alcohol Depend 126:1-2(257-62)2012 Nov 1

22240251. Wang YJ, Huang P, Ung A, Blendy JA, Liu-Chen LY, Reduced expression of the µ opioid receptor in some, but not all, brain regions in mice with OPRM1 A112G. Neuroscience 205:(178-84)2012 Mar 15

22204910. Béguin C, Potuzak J, Xu W, Liu-Chen LY, Streicher JM, Groer CE, Bohn LM, Carlezon WA Jr, Cohen BM, Differential signaling properties at the kappa opioid receptor of 12-epi-salvinorin A and its analogues. Bioorg Med Chem Lett 22:2(1023-6)2012 Jan 15

21864297. Huang P, Chen C, Mague SD, Blendy JA, Liu-Chen LY, A common single nucleotide polymorphism A118G of the µ opioid receptor alters its N-glycosylation and protein stability. Biochem J 441:1(379-86)2012 Jan 1

21841040. Wang YJ, Rasakham K, Huang P, Chudnovskaya D, Cowan A, Liu-Chen LY, Sex difference in ?-opioid receptor (KOPR)-mediated behaviors, brain region KOPR level and KOPR-mediated guanosine 5'-O-(3-[35S]thiotriphosphate) binding in the guinea pig. J Pharmacol Exp Ther 339:2(438-50)2011 Nov

21545805. Zhu J, Spencer TJ, Liu-Chen LY, Biederman J, Bhide PG, Methylphenidate and µ opioid receptor interactions: a pharmacological target for prevention of stimulant abuse. Neuropharmacology 61:1-2(283-92)2011 Jul-Aug

21388957. Chen C, Wang Y, Huang P, Liu-Chen LY, Effects of C-terminal modifications of GEC1 protein and gamma-aminobutyric acid type A (GABA(A)) receptor-associated protein (GABARAP), two microtubule-associated proteins, on kappa opioid receptor expression. J Biol Chem 286:17(15106-15)2011 Apr 29

20951148. Rasakham K, Liu-Chen LY, Sex differences in kappa opioid pharmacology. Life Sci 88:1-2(2-16)2011 Jan 3

20801035. Lee DY, Yang L, Xu W, Deng G, Guo L, Liu-Chen LY, Synthesis and biological evaluation of C-2 halogenated analogs of salvinorin A. Bioorg Med Chem Lett 20:19(5749-52)2010 Oct 1

20363293. Huang P, Liu-Chen LY, Kirby LG, Anxiety-like effects of SR141716-precipitated delta9-tetrahydrocannabinol withdrawal in mice in the elevated plus-maze. Neurosci Lett 475:3(165-8)2010 May 21

19733208. Huang P, Liu-Chen LY, Unterwald EM, Cowan A, Hyperlocomotion and paw tremors are two highly quantifiable signs of SR141716-precipitated withdrawal from delta9-tetrahydrocannabinol in C57BL/6 mice. Neurosci Lett 465:1(66-70)2009 Nov 6

19528658. Mague SD, Isiegas C, Huang P, Liu-Chen LY, Lerman C, Blendy JA, Mouse model of OPRM1 (A118G) polymorphism has sex-specific effects on drug-mediated behavior. Proc Natl Acad Sci U S A 106:26(10847-52)2009 Jun 30

19482639. Huang P, Liu-Chen LY, Detecting the mu opioid receptor in brain following SDS-PAGE with multiple approaches. Front Biosci (Elite Ed) 1:(220-7)2009 Jun 1

19416973. Wang Q, Liu-Chen LY, Traynor JR, Differential modulation of mu- and delta-opioid receptor agonists by endogenous RGS4 protein in SH-SY5Y cells. J Biol Chem 284:27(18357-67)2009 Jul 3

19373968. Brailoiu E, Dun SL, Gao X, Brailoiu GC, Li JG, Luo JJ, Yang J, Chang JK, Liu-Chen LY, Dun NJ, C-peptide of preproinsulin-like peptide 7: localization in the rat brain and activity in vitro. Neuroscience 159:2(492-500)2009 Mar 17

19273091. Chen Y, Liu-Chen LY, Chaperone-like effects of cell-permeant ligands on opioid receptors. Front Biosci (Landmark Ed) 14:(634-43)2009 Jan 1

19211245. Yang L, Xu W, Chen F, Liu-Chen LY, Ma Z, Lee DY, Synthesis and biological evaluation of C-12 triazole and oxadiazole analogs of salvinorin A. Bioorg Med Chem Lett 19:5(1301-4)2009 Mar 1

19147366. Béguin C, Duncan KK, Munro TA, Ho DM, Xu W, Liu-Chen LY, Carlezon WA Jr, Cohen BM, Modification of the furan ring of salvinorin A: identification of a selective partial agonist at the kappa opioid receptor. Bioorg Med Chem 17:3(1370-80)2009 Feb 1

19130621. Wang Y, Xu W, Huang P, Chavkin C, Van Bockstaele EJ, Liu-Chen LY, Effects of acute agonist treatment on subcellular distribution of kappa opioid receptor in rat spinal cord. J Neurosci Res 87:7(1695-702)2009 May 15

19001416. Chen Y, Chen C, Kotsikorou E, Lynch DL, Reggio PH, Liu-Chen LY, GEC1-kappa opioid receptor binding involves hydrophobic interactions: GEC1 has chaperone-like effect. J Biol Chem 284:3(1673-85)2009 Jan 16

18950900. Woodruff-Pak DS, Lehr MA, Li JG, Liu-Chen LY, Young and older good learners have higher levels of brain nicotinic receptor binding. Neurobiol Aging 31:6(1032-43)2010 Jun

18930741. Wang Y, Van Bockstaele EJ, Liu-Chen LY, In vivo trafficking of endogenous opioid receptors. Life Sci 83:21-22(693-9)2008 Nov 21

18925386. Inan S, Lee DY, Liu-Chen LY, Cowan A, Comparison of the diuretic effects of chemically diverse kappa opioid agonists in rats: nalfurafine, U50,488H, and salvinorin A. Naunyn Schmiedebergs Arch Pharmacol 379:3(263-70)2009 Mar

18830156. Ma ZZ, Xu W, Jensen NH, Roth BL, Liu-Chen LY, Lee DY, Isoquinoline alkaloids isolated from Corydalis yanhusuo and their binding affinities at the dopamine D1 receptor. Molecules 13:9(2303-12)2008 Sep 25

18256594. Li JG, Lehr M, Liu-Chen LY, Woodruff-Pak DS, Nicotinic acetylcholine receptors and modulation of learning in 4- and 27-month-old rabbits. Neuropsychopharmacology 33:12(2820-30)2008 Nov

18212250. Li JG, Haines DS, Liu-Chen LY, Agonist-promoted Lys63-linked polyubiquitination of the human kappa-opioid receptor is involved in receptor down-regulation. Mol Pharmacol 73:4(1319-30)2008 Apr

18166466. Ma Z, Xu W, Liu-Chen LY, Lee DY, Novel coumarin glycoside and phenethyl vanillate from Notopterygium forbesii and their binding affinities for opioid and dopamine receptors. Bioorg Med Chem 16:6(3218-23)2008 Mar 15

18089845. Wang Y, Chen Y, Xu W, Lee DY, Ma Z, Rawls SM, Cowan A, Liu-Chen LY, 2-Methoxymethyl-salvinorin B is a potent kappa opioid receptor agonist with longer lasting action in vivo than salvinorin A. J Pharmacol Exp Ther 324:3(1073-83)2008 Mar

17981041. Munro TA, Duncan KK, Xu W, Wang Y, Liu-Chen LY, Carlezon WA Jr, Cohen BM, Béguin C, Standard protecting groups create potent and selective kappa opioids: salvinorin B alkoxymethyl ethers. Bioorg Med Chem 16:3(1279-86)2008 Feb 1

17980352. Huang P, Xu W, Yoon SI, Chen C, Chong PL, Unterwald EM, Liu-Chen LY, Agonist treatment did not affect association of mu opioid receptors with lipid rafts and cholesterol reduction had opposite effects on the receptor-mediated signaling in rat brain and CHO cells. Brain Res 1184:(46-56)2007 Dec 12

17980152. Huang P, Chen C, Xu W, Yoon SI, Unterwald EM, Pintar JE, Wang Y, Chong PL, Liu-Chen LY, Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor. Biochem Biophys Res Commun 365:1(82-8)2008 Jan 4

17951511. Béguin C, Potter DN, Dinieri JA, Munro TA, Richards MR, Paine TA, Berry L, Zhao Z, Roth BL, Xu W, Liu-Chen LY, Carlezon WA Jr, Cohen BM, N-methylacetamide analog of salvinorin A: a highly potent and selective kappa-opioid receptor agonist with oral efficacy. J Pharmacol Exp Ther 324:1(188-95)2008 Jan

17711303. Li JG, Chen C, Liu-Chen LY, N-Glycosylation of the human kappa opioid receptor enhances its stability but slows its trafficking along the biosynthesis pathway. Biochemistry 46:38(10960-70)2007 Sep 25

17433292. Xie Z, Li Z, Guo L, Ye C, Li J, Yu X, Yang H, Wang Y, Chen C, Zhang D, Liu-Chen LY, Regulator of G protein signaling proteins differentially modulate signaling of mu and delta opioid receptors. Eur J Pharmacol 565:1-3(45-53)2007 Jun 22

17316701. Chen Y, Chen C, Liu-Chen LY, Dynorphin peptides differentially regulate the human kappa opioid receptor. Life Sci 80:15(1439-48)2007 Mar 20

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Laboratory Personnel:


Research Technologist: Chongguang Che, cgggc@temple.edu

Associate Scientist: Peng Huang, PhD, peng.huang@temple.edu
Postdoctoral Fellow: Yujun Wang, PhD, yujun@temple.edu
Graduate Students: Yi-Ting Chiu, rubisco85@gmail.com

                                     Kelly DiMattio, tuc21238@temple.edu


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