about | Maps & Directions | contact | admissions | faculty | alumni & development | library | Tech Support Center | dean's office | Policies & Procedures

FAculty directory

Back to alphabetical index


Lee Liu-chen, PhDLee-Yuan Liu-Chen, PhD


Professor, Pharmacology

Telephone:  215-707-4188

Fax:  215-707-6661

Email: lee-yuan.liu-chen@temple.edu


Department of Pharmacology

Center for Substance Abuse Research


Educational Background:


BS, National Taiwan University, Taipei, Taiwan 1976


MS, University of Minnesota (Mayo Clinic), Rochester, MN 1980


PhD, Massachusetts Institue of Technology, Cambridge, MA 1983


Postdoctoral Fellowship, Massachusetts General Hospital/Harvard Medical School, Boston, MA 1984

Return to top



Research Interests:


  • Biochemistry and molecular and cellular pharmacology of opioid receptors.
  • Mechanisms underlying drug abuse.


Return to top




Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

26330078. Lee DY, Deng G, Ma Z, Xu W, Yang L, Liu J, Dai R, Liu-Chen LY, Synthesis and biological evaluation of 2-alkyl-2-methoxymethyl-salvinorin ethers as selective ?-opioid receptor agonists. Bioorg Med Chem Lett 25:20(4689-92)2015 Oct 15

26057692. DiMattio KM, Ehlert FJ, Liu-Chen LY, Intrinsic relative activities of ? opioid agonists in activating Ga proteins and internalizing receptor: Differences between human and mouse receptors. Eur J Pharmacol 761:(235-44)2015 Aug 15

25481857. DiMattio KM, Chen C, Shi L, Liu-Chen LY, K3036·58 in the µ opioid (MOP) receptor is important in conferring selectivity for covalent binding of ß-funaltrexamine (ß-FNA). Eur J Pharmacol 748:(93-100)2015 Feb 5

25293322. Huang P, Chen C, Liu-Chen LY, Detection of mu opioid receptor (MOPR) and its glycosylation in rat and mouse brains by western blot with anti-µC, an affinity-purified polyclonal anti-MOPR antibody. Methods Mol Biol 1230:(141-54)2015

25293321. Chen C, Huang P, Liu-Chen LY, Identification and verification of proteins interacting with the kappa opioid receptor (KOPR). Methods Mol Biol 1230:(129-40)2015

24503508. Dimattio KM, Yakovleva TV, Aldrich JV, Cowan A, Liu-Chen LY, Zyklophin, a short-acting kappa opioid antagonist, induces scratching in mice. Neurosci Lett 563:(155-9)2014 Mar 20

24454571. Munro TA, Xu W, Ho DM, Liu-Chen LY, Cohen BM, Studies toward bivalent ? opioids derived from salvinorin A: heteromethylation of the furan ring reduces affinity. Beilstein J Org Chem 9:(2916-24)2013 Dec 20

24080315. Xu W, Wang Y, Ma Z, Chiu YT, Huang P, Rasakham K, Unterwald E, Lee DY, Liu-Chen LY, L-isocorypalmine reduces behavioral sensitization and rewarding effects of cocaine in mice by acting on dopamine receptors. Drug Alcohol Depend 133:2(693-703)2013 Dec 1

23856011. Huang P, Chiu YT, Chen C, Wang Y, Liu-Chen LY, A G protein-coupled receptor (GPCR) in red: live cell imaging of the kappa opioid receptor-tdTomato fusion protein (KOPR-tdT) in neuronal cells. J Pharmacol Toxicol Methods 68:3(340-5)2013 Nov-Dec

23624231. Xu W, Chen C, Li JG, Dimattio K, Wang Y, Unterwald E, Liu-Chen LY, PKA and ERK1/2 are involved in dopamine D1 receptor-induced heterologous desensitization of the d opioid receptor. Life Sci 92:23(1101-9)2013 Jun 21

23446450. Van't Veer A, Bechtholt AJ, Onvani S, Potter D, Wang Y, Liu-Chen LY, Schütz G, Chartoff EH, Rudolph U, Cohen BM, Carlezon WA Jr, Ablation of kappa-opioid receptors from brain dopamine neurons has anxiolytic-like effects and enhances cocaine-induced plasticity. Neuropsychopharmacology 38:8(1585-97)2013 Jul

23086943. Schattauer SS, Miyatake M, Shankar H, Zietz C, Levin JR, Liu-Chen LY, Gurevich VV, Rieder MJ, Chavkin C, Ligand directed signaling differences between rodent and human ?-opioid receptors. J Biol Chem 287:50(41595-607)2012 Dec 7

22989890. Li JG, Chen C, Huang P, Wang Y, Liu-Chen LY, 14-3-3? Protein regulates anterograde transport of the human ?-opioid receptor (hKOPR). J Biol Chem 287:45(37778-92)2012 Nov 2

22863678. Rasakham K, McGillivray KL, Liu-Chen LY, Sex differences in U50,488H-induced phosphorylation of p44/42 mitogen-activated protein kinase in the guinea pig brain. Neuroscience 223:(447-56)2012 Oct 25

22862850. Wang YJ, Huang P, Blendy JA, Liu-Chen LY, Brain region- and sex-specific alterations in DAMGO-stimulated [(35) S]GTP?S binding in mice with Oprm1 A112G. Addict Biol 19:3(354-61)2014 May

22736766. Rives ML, Rossillo M, Liu-Chen LY, Javitch JA, 6'-Guanidinonaltrindole (6'-GNTI) is a G protein-biased ?-opioid receptor agonist that inhibits arrestin recruitment. J Biol Chem 287:32(27050-4)2012 Aug 3

22652295. Lisek R, Xu W, Yuvasheva E, Chiu YT, Reitz AB, Liu-Chen LY, Rawls SM, Mephedrone ('bath salt') elicits conditioned place preference and dopamine-sensitive motor activation. Drug Alcohol Depend 126:1-2(257-62)2012 Nov 1

22240251. Wang YJ, Huang P, Ung A, Blendy JA, Liu-Chen LY, Reduced expression of the µ opioid receptor in some, but not all, brain regions in mice with OPRM1 A112G. Neuroscience 205:(178-84)2012 Mar 15

22204910. Béguin C, Potuzak J, Xu W, Liu-Chen LY, Streicher JM, Groer CE, Bohn LM, Carlezon WA Jr, Cohen BM, Differential signaling properties at the kappa opioid receptor of 12-epi-salvinorin A and its analogues. Bioorg Med Chem Lett 22:2(1023-6)2012 Jan 15

21864297. Huang P, Chen C, Mague SD, Blendy JA, Liu-Chen LY, A common single nucleotide polymorphism A118G of the µ opioid receptor alters its N-glycosylation and protein stability. Biochem J 441:1(379-86)2012 Jan 1

21841040. Wang YJ, Rasakham K, Huang P, Chudnovskaya D, Cowan A, Liu-Chen LY, Sex difference in ?-opioid receptor (KOPR)-mediated behaviors, brain region KOPR level and KOPR-mediated guanosine 5'-O-(3-[35S]thiotriphosphate) binding in the guinea pig. J Pharmacol Exp Ther 339:2(438-50)2011 Nov

21545805. Zhu J, Spencer TJ, Liu-Chen LY, Biederman J, Bhide PG, Methylphenidate and µ opioid receptor interactions: a pharmacological target for prevention of stimulant abuse. Neuropharmacology 61:1-2(283-92)2011 Jul-Aug

21388957. Chen C, Wang Y, Huang P, Liu-Chen LY, Effects of C-terminal modifications of GEC1 protein and gamma-aminobutyric acid type A (GABA(A)) receptor-associated protein (GABARAP), two microtubule-associated proteins, on kappa opioid receptor expression. J Biol Chem 286:17(15106-15)2011 Apr 29

20951148. Rasakham K, Liu-Chen LY, Sex differences in kappa opioid pharmacology. Life Sci 88:1-2(2-16)2011 Jan 3

20801035. Lee DY, Yang L, Xu W, Deng G, Guo L, Liu-Chen LY, Synthesis and biological evaluation of C-2 halogenated analogs of salvinorin A. Bioorg Med Chem Lett 20:19(5749-52)2010 Oct 1

20363293. Huang P, Liu-Chen LY, Kirby LG, Anxiety-like effects of SR141716-precipitated delta9-tetrahydrocannabinol withdrawal in mice in the elevated plus-maze. Neurosci Lett 475:3(165-8)2010 May 21

19733208. Huang P, Liu-Chen LY, Unterwald EM, Cowan A, Hyperlocomotion and paw tremors are two highly quantifiable signs of SR141716-precipitated withdrawal from delta9-tetrahydrocannabinol in C57BL/6 mice. Neurosci Lett 465:1(66-70)2009 Nov 6

19528658. Mague SD, Isiegas C, Huang P, Liu-Chen LY, Lerman C, Blendy JA, Mouse model of OPRM1 (A118G) polymorphism has sex-specific effects on drug-mediated behavior. Proc Natl Acad Sci U S A 106:26(10847-52)2009 Jun 30

19482639. Huang P, Liu-Chen LY, Detecting the mu opioid receptor in brain following SDS-PAGE with multiple approaches. Front Biosci (Elite Ed) 1:(220-7)2009 Jun 1

19416973. Wang Q, Liu-Chen LY, Traynor JR, Differential modulation of mu- and delta-opioid receptor agonists by endogenous RGS4 protein in SH-SY5Y cells. J Biol Chem 284:27(18357-67)2009 Jul 3

19373968. Brailoiu E, Dun SL, Gao X, Brailoiu GC, Li JG, Luo JJ, Yang J, Chang JK, Liu-Chen LY, Dun NJ, C-peptide of preproinsulin-like peptide 7: localization in the rat brain and activity in vitro. Neuroscience 159:2(492-500)2009 Mar 17

19273091. Chen Y, Liu-Chen LY, Chaperone-like effects of cell-permeant ligands on opioid receptors. Front Biosci (Landmark Ed) 14:(634-43)2009 Jan 1

19211245. Yang L, Xu W, Chen F, Liu-Chen LY, Ma Z, Lee DY, Synthesis and biological evaluation of C-12 triazole and oxadiazole analogs of salvinorin A. Bioorg Med Chem Lett 19:5(1301-4)2009 Mar 1

19147366. Béguin C, Duncan KK, Munro TA, Ho DM, Xu W, Liu-Chen LY, Carlezon WA Jr, Cohen BM, Modification of the furan ring of salvinorin A: identification of a selective partial agonist at the kappa opioid receptor. Bioorg Med Chem 17:3(1370-80)2009 Feb 1

19130621. Wang Y, Xu W, Huang P, Chavkin C, Van Bockstaele EJ, Liu-Chen LY, Effects of acute agonist treatment on subcellular distribution of kappa opioid receptor in rat spinal cord. J Neurosci Res 87:7(1695-702)2009 May 15

19001416. Chen Y, Chen C, Kotsikorou E, Lynch DL, Reggio PH, Liu-Chen LY, GEC1-kappa opioid receptor binding involves hydrophobic interactions: GEC1 has chaperone-like effect. J Biol Chem 284:3(1673-85)2009 Jan 16

18950900. Woodruff-Pak DS, Lehr MA, Li JG, Liu-Chen LY, Young and older good learners have higher levels of brain nicotinic receptor binding. Neurobiol Aging 31:6(1032-43)2010 Jun

18930741. Wang Y, Van Bockstaele EJ, Liu-Chen LY, In vivo trafficking of endogenous opioid receptors. Life Sci 83:21-22(693-9)2008 Nov 21

18925386. Inan S, Lee DY, Liu-Chen LY, Cowan A, Comparison of the diuretic effects of chemically diverse kappa opioid agonists in rats: nalfurafine, U50,488H, and salvinorin A. Naunyn Schmiedebergs Arch Pharmacol 379:3(263-70)2009 Mar

18830156. Ma ZZ, Xu W, Jensen NH, Roth BL, Liu-Chen LY, Lee DY, Isoquinoline alkaloids isolated from Corydalis yanhusuo and their binding affinities at the dopamine D1 receptor. Molecules 13:9(2303-12)2008 Sep 25

18256594. Li JG, Lehr M, Liu-Chen LY, Woodruff-Pak DS, Nicotinic acetylcholine receptors and modulation of learning in 4- and 27-month-old rabbits. Neuropsychopharmacology 33:12(2820-30)2008 Nov

18212250. Li JG, Haines DS, Liu-Chen LY, Agonist-promoted Lys63-linked polyubiquitination of the human kappa-opioid receptor is involved in receptor down-regulation. Mol Pharmacol 73:4(1319-30)2008 Apr

18166466. Ma Z, Xu W, Liu-Chen LY, Lee DY, Novel coumarin glycoside and phenethyl vanillate from Notopterygium forbesii and their binding affinities for opioid and dopamine receptors. Bioorg Med Chem 16:6(3218-23)2008 Mar 15

18089845. Wang Y, Chen Y, Xu W, Lee DY, Ma Z, Rawls SM, Cowan A, Liu-Chen LY, 2-Methoxymethyl-salvinorin B is a potent kappa opioid receptor agonist with longer lasting action in vivo than salvinorin A. J Pharmacol Exp Ther 324:3(1073-83)2008 Mar

17981041. Munro TA, Duncan KK, Xu W, Wang Y, Liu-Chen LY, Carlezon WA Jr, Cohen BM, Béguin C, Standard protecting groups create potent and selective kappa opioids: salvinorin B alkoxymethyl ethers. Bioorg Med Chem 16:3(1279-86)2008 Feb 1

17980352. Huang P, Xu W, Yoon SI, Chen C, Chong PL, Unterwald EM, Liu-Chen LY, Agonist treatment did not affect association of mu opioid receptors with lipid rafts and cholesterol reduction had opposite effects on the receptor-mediated signaling in rat brain and CHO cells. Brain Res 1184:(46-56)2007 Dec 12

17980152. Huang P, Chen C, Xu W, Yoon SI, Unterwald EM, Pintar JE, Wang Y, Chong PL, Liu-Chen LY, Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor. Biochem Biophys Res Commun 365:1(82-8)2008 Jan 4

17951511. Béguin C, Potter DN, Dinieri JA, Munro TA, Richards MR, Paine TA, Berry L, Zhao Z, Roth BL, Xu W, Liu-Chen LY, Carlezon WA Jr, Cohen BM, N-methylacetamide analog of salvinorin A: a highly potent and selective kappa-opioid receptor agonist with oral efficacy. J Pharmacol Exp Ther 324:1(188-95)2008 Jan

17711303. Li JG, Chen C, Liu-Chen LY, N-Glycosylation of the human kappa opioid receptor enhances its stability but slows its trafficking along the biosynthesis pathway. Biochemistry 46:38(10960-70)2007 Sep 25

17433292. Xie Z, Li Z, Guo L, Ye C, Li J, Yu X, Yang H, Wang Y, Chen C, Zhang D, Liu-Chen LY, Regulator of G protein signaling proteins differentially modulate signaling of mu and delta opioid receptors. Eur J Pharmacol 565:1-3(45-53)2007 Jun 22

Return to top




Laboratory Personnel:


Research Technologist: Chongguang Che, cgggc@temple.edu

Associate Scientist: Peng Huang, PhD, peng.huang@temple.edu
Postdoctoral Fellow: Yujun Wang, PhD, yujun@temple.edu
Graduate Students: Yi-Ting Chiu, rubisco85@gmail.com

                                     Kelly DiMattio, tuc21238@temple.edu


Return to top