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T. Dianne Langford, PhDT. Dianne Langford, PhD


Associate Professor, Neuroscience

Associate Professor, Neurovirology

Location: Room 750 MERB

Telephone:  215-707-5487

Fax:  215-707-4888

Email: tdl@temple.edu


Department of Neuroscience

Center for Neurovirology


Educational Background:


PhD, Biological Sciences, University of Alabama, Tuscaloosa, AL, 1996


Postdoctoral Fellowship, University of California San Diego School of Medicine, La Jolla, CA, 2001


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Research Interests:


Studies in Dr. Langford’s lab focus on how cells of the brain respond to HIV infection of the central nervous system (CNS) and to other CNS-specific challenges including substance abuse and abnormal protein processing.


. We are interested in CNS cell response to the harmful effects of HIV infection, drugs of abuse, and other disorders associated with CNS dysfunction.  Specifically, we are focusing on mechanisms by which host cells attempt to preserve or restore normal cell functioning.  In earlier studies, we reported CNS cell-type specific protective responses to HIV-associated CNS challenge (1, 2).


More recent studies from our lab report the production of a protein called PINCH by neurons in the brains of HIV infected patients (3).  These results are particularly interesting since PINCH is undetectable in normal (un-diseased) brains.  In health, PINCH is expressed during development to maintain cellular polarity and communication with the extracellular matrix.  From our data, it appears that PINCH expression is recalled to repair or rescue damaged neurons.  To address potential triggers for PINCH induction in HIV patients’ brains we are using an in vitro system that mimics some aspects of HIV infection of the CNS (4).  Our data show that HIV proteins and inflammatory factors produced in response to HIV infection of CNS prompt PINCH expression.  Interestingly, sequestering PINCH using antibody-mediated mobility-disruption resulted in fewer and shorter neurite processes following treatment with the inflammatory factor, TNF-α, indicating that not only is PINCH increased upon challenge, but that it must also be free to migrate throughout the cell to maintain neurite extensions.


Individuals who suffer from substance abuse disorder are at high risk for becoming infected by HIV.  Drugs of abuse such as cocaine, methamphetamine, heroin and alcohol continue to account for a significant percentage of CNS dysfunctions treated in the clinic.  In this context, our lab is studying the potential interactions of drugs of abuse, HIV and CNS cell signaling in the context of neuroplasticity and neuronal recovery (5).


Cited references:

  1. Hashimoto, M., Sagara, Y., Langford, D., Everall, I. P., Mallory, M., Everson, A., Digicaylioglu, M., and Masliah, E. (2002) The Journal of biological chemistry 277(36), 32985-32991
  2. Langford, D., Hurford, R., Hashimoto, M., Digicaylioglu, M., and Masliah, E. (2005) BMC neuroscience 6(1), 8
  3. Rearden, A., Hurford, RG, Luu, N, Kieu, E, Sandoval, M, Perez-Liz, G, Del Valle, L, Powell, H, Langford TD. (2008) Journal of neuroscience research 86(11), 2535-2542
  4. Jatiani, A., Pannizzo, Paola, Gualco, Elisa, Del Valle, Luis, Langford, Dianne. (2010) Journal of Neuroimmune Pharmacology 6(3), 330-340
  5. Langford D, Baron D, Joy J, Del Valle L, and J., S. (2010) Psychoneuroendocrinology. 36(5), 710-719

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Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

17180161. Ellis R, Langford D, Masliah E, HIV and antiretroviral therapy in the brain: neuronal injury and repair. Nat Rev Neurosci 8:1(33-44)2007 Jan

17060582. Chana G, Everall IP, Crews L, Langford D, Adame A, Grant I, Cherner M, Lazzaretto D, Heaton R, Ellis R, Masliah E, Cognitive deficits and degeneration of interneurons in HIV+ methamphetamine users. Neurology 67:8(1486-9)2006 Oct 24

16798671. Langford D, Marquie-Beck J, de Almeida S, Lazzaretto D, Letendre S, Grant I, McCutchan JA, Masliah E, Ellis RJ, Relationship of antiretroviral treatment to postmortem brain tissue viral load in human immunodeficiency virus-infected patients. J Neurovirol 12:2(100-7)2006 Apr

15960237. Tadesse T, Langford D, Manji K, Mehari E, Patterns of neuroAIDS in Africa. J Neurovirol 11 Suppl 1:(22-6)2005

15765804. Langford D, Grigorian A, Hurford R, Adame A, Crews L, Masliah E, The role of mitochondrial alterations in the combined toxic effects of human immunodeficiency virus Tat protein and methamphetamine on calbindin positive-neurons. J Neurovirol 10:6(327-37)2004 Dec

15689238. Langford D, Hurford R, Hashimoto M, Digicaylioglu M, Masliah E, Signalling crosstalk in FGF2-mediated protection of endothelial cells from HIV-gp120. BMC Neurosci 6:(8)2005 Feb 2

15579294. Masliah E, Roberts ES, Langford D, Everall I, Crews L, Adame A, Rockenstein E, Fox HS, Patterns of gene dysregulation in the frontal cortex of patients with HIV encephalitis. J Neuroimmunol 157:1-2(163-75)2004 Dec

15535131. Langford D, Grigorian A, Hurford R, Adame A, Ellis RJ, Hansen L, Masliah E, Altered P-glycoprotein expression in AIDS patients with HIV encephalitis. J Neuropathol Exp Neurol 63:10(1038-47)2004 Oct

15081264. Carlson KA, Limoges J, Pohlman GD, Poluektova LY, Langford D, Masliah E, Ikezu T, Gendelman HE, OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis. J Neuroimmunol 150:1-2(186-98)2004 May

14769347. Langford D, Masliah E, The emerging role of infectious pathogens in neurodegenerative diseases. Exp Neurol 184:2(553-5)2003 Dec

14657756. Langford D, Adame A, Grigorian A, Grant I, McCutchan JA, Ellis RJ, Marcotte TD, Masliah E, Patterns of selective neuronal damage in methamphetamine-user AIDS patients. J Acquir Immune Defic Syndr 34:5(467-74)2003 Dec 15

12498789. Everall IP, Bell C, Mallory M, Langford D, Adame A, Rockestein E, Masliah E, Lithium ameliorates HIV-gp120-mediated neurotoxicity. Mol Cell Neurosci 21:3(493-501)2002 Nov

12476355. Langford D, Masliah E, Role of trophic factors on neuroimmunity in neurodegenerative infectious diseases. J Neurovirol 8:6(625-38)2002 Dec

12238761. Chismar JD, Mondala T, Fox HS, Roberts E, Langford D, Masliah E, Salomon DR, Head SR, Analysis of result variability from high-density oligonucleotide arrays comparing same-species and cross-species hybridizations. Biotechniques 33:3(516-8, 520, 522 passim)2002 Sep

12095987. Hashimoto M, Sagara Y, Langford D, Everall IP, Mallory M, Everson A, Digicaylioglu M, Masliah E, Fibroblast growth factor 1 regulates signaling via the glycogen synthase kinase-3beta pathway. Implications for neuroprotection. J Biol Chem 277:36(32985-91)2002 Sep 6

12044982. Langford D, Sanders VJ, Mallory M, Kaul M, Masliah E, Expression of stromal cell-derived factor 1alpha protein in HIV encephalitis. J Neuroimmunol 127:1-2(115-26)2002 Jun

11414473. Langford D, Masliah E, Crosstalk between components of the blood brain barrier and cells of the CNS in microglial activation in AIDS. Brain Pathol 11:3(306-12)2001 Jul

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