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Barbara Krynska, MS, PhD


Barbara Krynska, MS, PhD


Assistant Professor, Neurology

Assistant Professor, Shriners Hospitals Pediatric Research Center

Telephone: 215-707-7233

Email: krynskb@tuhs.temple.edu


Department of Neurology

Shriners Hospitals Pediatric Research Center


Educational Background:


MS, Medical University of Warsaw, Poland, 1992


PhD, Medical University of Silesia, Poland, 1998


Visiting scholar, Thomas Jefferson University, Philadelphia, PA, 1995-1996


Visiting scholar, Allegheny University of the Health Sciences, Philadelphia, PA, 1996-1998


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professional affiliations:

  • Society for Neuroscience
  • International Society for NeuroVirology

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Dr. Krynska is studying the basic biology of adult-derived stem cells and testing their potential for repair of the injured nervous system. She is interested in the characterization, behavior, and plasticity of neural and mesenchymal stem cells and the understanding of environmental signals that govern the fate of these cells. This is accomplished by manipulating the tissue culture environment in vitro and by transplantations of stem cells in vivo. One of the recent models that she is working on is a retinoic acid-induced model of spina bifida in fetal rats that mimics human myelomeningocele, a severe form of spina bifida. Dr. Krynska is investigating the role of prenatal transplantation of mesenchymal stem cells on repairing the spina bifida defects in this model. A novel aspect of the research is to deliver mesenchymal cells after seeding them on a frame/scaffolding to engineer tissue for repair in situ. Another way of creating a scaffold for stem cells is to build a vascular framework, which is a key component of the in vivo environment and a necessary precondition for the successful engraftment of functional engineered tissue after injury. In collaboration with Drs. Kiani and Wang in the Department of Mechanical Engineering, Dr. Krynska is utilizing immunoliposomes specifically targeting cell adhesion molecules to deliver proangiogenic compounds to injured tissue after myocardial infarction in addition to implantation of mesenchymal stem cells.

Dr. Krynska is also interested in exploring the role of stem cells in the origin and therapy of primary brain tumors. She is utilizing the oncoprotein of the human polyomavirus JCV, T-antigen, as a tool to delineate the role of stem/progenitor cells in the genesis of brain tumors. The ultimate goal of this study is to utilize these cells to explore the possible mechanisms of transformation of adult stem cells into cancer stem cells and to eventually apply this information for controlling oncogenic cell transformation. In addition, this information will allow for the determination of the safety and preclinical efficacy of stem cells in regenerative medicine. In collaboration with Drs. Gordon and Khalili in the Department of Neuroscience, Dr. Krynska is applying the JCV T-antigen induced stem cell model to study the pathogenesis of JCV- associated diseases.


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Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

26555149. Deosarkar SP, Prabhakarpandian B, Wang B, Sheffield JB, Krynska B, Kiani MF, A Novel Dynamic Neonatal Blood-Brain Barrier on a Chip. PLoS One 10:11(e0142725)2015

25498106. Smith GM, Krynska B, Myelomeningocele: How we can improve the assessment of the most severe form of spina bifida. Brain Res 1619:(84-90)2015 Sep 4

24954432. Barbe MF, Adiga R, Gordiienko O, Pleshko N, Selzer ME, Krynska B, Micro-computed tomography assessment of vertebral column defects in retinoic acid-induced rat model of myelomeningocele. Birth Defects Res A Clin Mol Teratol 100:6(453-62)2014 Jun

24941463. Tang Y, Gan X, Cheheltani R, Curran E, Lamberti G, Krynska B, Kiani MF, Wang B, Targeted delivery of vascular endothelial growth factor improves stem cell therapy in a rat myocardial infarction model. Nanomedicine 10:8(1711-8)2014 Nov

23975823. Azizi SA, Krynska B, Derivation of neuronal cells from fetal normal human astrocytes (NHA). Methods Mol Biol 1078:(89-96)2013

23805194. Gordon J, Sariyer IK, De La Fuente-Granada M, Augelli BJ, Otte J, Azizi SA, Amini S, Khalili K, Krynska B, Neural Crest Cells Isolated from the Bone Marrow of Transgenic Mice Express JCV T-Antigen. PLoS One 8:6(e65947)2013

22573347. Imitola J, Pitt K, Peoples JL, Krynska B, Sheikh H, Kesari S, Azizi SA, Multifocal CNS infiltration of chronic lymphocytic leukemia in the form of small-cell solid metastatic lesions. J Neurooncol 109:1(213-5)2012 Aug

22034072. Merabova N, Kaminski R, Krynska B, Amini S, Khalili K, Darbinyan A, JCV agnoprotein-induced reduction in CXCL5/LIX secretion by oligodendrocytes is associated with activation of apoptotic signaling in neurons. J Cell Physiol 227:8(3119-27)2012 Aug

20190567. Del Valle L, Piņa-Oviedo S, Perez-Liz G, Augelli BJ, Azizi SA, Khalili K, Gordon J, Krynska B, Bone marrow-derived mesenchymal stem cells undergo JCV T-antigen mediated transformation and generate tumors with neuroectodermal characteristics. Cancer Biol Ther 9:4(286-94)2010 Feb

23487404. Knight LC, Romano JE, Krynska B, Faro S, Mohamed FB, Gordon J, Binding and Internalization of Iron Oxide Nanoparticles Targeted to Nuclear Oncoprotein. J Mol Biomark Diagn 1:1()2010

19452279. Rosano JM, Tousi N, Scott RC, Krynska B, Rizzo V, Prabhakarpandian B, Pant K, Sundaram S, Kiani MF, A physiologically realistic in vitro model of microvascular networks. Biomed Microdevices 11:5(1051-7)2009 Oct

19216795. Rieske P, Golanska E, Zakrzewska M, Piaskowski S, Hulas-Bigoszewska K, Wolanczyk M, Szybka M, Witusik-Perkowska M, Jaskolski DJ, Zakrzewski K, Biernat W, Krynska B, Liberski PP, Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors. BMC Cancer 9:(54)2009 Feb 14

19061885. Rieske P, Augelli BJ, Stawski R, Gaughan J, Azizi SA, Krynska B, A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells. Exp Cell Res 315:3(462-73)2009 Feb 1

18638414. Witusik M, Piaskowski S, Hulas-Bigoszewska K, Zakrzewska M, Gresner SM, Azizi SA, Krynska B, Liberski PP, Rieske P, Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells. BMC Biotechnol 8:(56)2008 Jul 19

18426386. Scott RC, Crabbe D, Krynska B, Ansari R, Kiani MF, Aiming for the heart: targeted delivery of drugs to diseased cardiac tissue. Expert Opin Drug Deliv 5:4(459-70)2008 Apr

17996224. Witusik M, Gresner SM, Hulas-Bigoszewska K, Krynska B, Azizi SA, Liberski PP, Brown P, Rieske P, Neuronal and astrocytic cells, obtained after differentiation of human neural GFAP-positive progenitors, present heterogeneous expression of PrPc. Brain Res 1186:(65-73)2007 Dec

17241264. Rieske P, Azizi SA, Augelli B, Gaughan J, Krynska B, A population of human brain parenchymal cells express markers of glial, neuronal and early neural cells and differentiate into cells of neuronal and glial lineages. Eur J Neurosci 25:1(31-7)2007 Jan

16351691. Rieske P, Krynska B, Azizi SA, Human fibroblast-derived cell lines have characteristics of embryonic stem cells and cells of neuro-ectodermal origin. Differentiation 73:9-10(474-83)2005 Dec

11303381. Khalili K, Croul S, Del Valle L, Krynska B, Gordon J, Oncogenic potential of human neurotropic virus: laboratory and clinical observations. Isr Med Assoc J 3:3(210-5)2001 Mar

11243899. Jasani B, Cristaudo A, Emri SA, Gazdar AF, Gibbs A, Krynska B, Miller C, Mutti L, Radu C, Tognon M, Procopio A, Association of SV40 with human tumours. Semin Cancer Biol 11:1(49-61)2001 Feb

11117551. Del Valle L, Azizi SA, Krynska B, Enam S, Croul SE, Khalili K, Reactivation of human neurotropic JC virus expressing oncogenic protein in a recurrent glioblastoma multiforme. Ann Neurol 48:6(932-6)2000 Dec

10936089. Krynska B, Del Valle L, Gordon J, Otte J, Croul S, Khalili K, Identification of a novel p53 mutation in JCV-induced mouse medulloblastoma. Virology 274:1(65-74)2000 Aug 15

10500209. Krynska B, Del Valle L, Croul S, Gordon J, Katsetos CD, Carbone M, Giordano A, Khalili K, Detection of human neurotropic JC virus DNA sequence and expression of the viral oncogenic protein in pediatric medulloblastomas. Proc Natl Acad Sci U S A 96:20(11519-24)1999 Sep 28

10209983. Khalili K, Krynska B, Del Valle L, Katsetos CD, Croul S, Medulloblastomas and the human neurotropic polyomavirus JC virus. Lancet 353:9159(1152-3)1999 Apr 3

10082081. Tretiakova A, Krynska B, Gordon J, Khalili K, Human neurotropic JC virus early protein deregulates glial cell cycle pathway and impairs cell differentiation. J Neurosci Res 55:5(588-99)1999 Mar 1

9926918. Krynska B, Otte J, Franks R, Khalili K, Croul S, Human ubiquitous JCV(CY) T-antigen gene induces brain tumors in experimental animals. Oncogene 18:1(39-46)1999 Jan 7

9789434. Krynska B, Lewin-Kowalik J, Sieron AL, [Disturbance of the normal cell cycle by T antigens of SV40 viruses and JC cause some tumors]. Postepy Hig Med Dosw 52:3(237-57)1998

9788590. Testa JR, Carbone M, Hirvonen A, Khalili K, Krynska B, Linnainmaa K, Pooley FD, Rizzo P, Rusch V, Xiao GH, A multi-institutional study confirms the presence and expression of simian virus 40 in human malignant mesotheliomas. Cancer Res 58:20(4505-9)1998 Oct 15

9776230. Gordon J, Krynska B, Otte J, Houff SA, Khalili K, Oncogenic potential of human neurotropic papovavirus, JCV, in CNS. Dev Biol Stand 94:(93-101)1998

9473521. Steplewski A, Krynska B, Tretiakova A, Haas S, Khalili K, Amini S, MyEF-3, a developmentally controlled brain-derived nuclear protein which specifically interacts with myelin basic protein proximal regulatory sequences. Biochem Biophys Res Commun 243:1(295-301)1998 Feb 4

9328827. Krynska B, Gordon J, Otte J, Franks R, Knobler R, DeLuca A, Giordano A, Khalili K, Role of cell cycle regulators in tumor formation in transgenic mice expressing the human neurotropic virus, JCV, early protein. J Cell Biochem 67:2(223-30)1997 Nov 1

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