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Linda Knight, PhD

Professor, Radiology

Associate Professor, Sol Sherry Thrombosis Research Center

Telephone:  215-707-4940

Fax:  215-707-8110

Email: lknight@temple.edu

Department of Radiology

Sol Sherry Thrombosis Research Center

University of California, Davis, CA

B.S., 1972 Chemistry (honors)

 

Washington University, St. Louis, MO

M.A., 1974 Nuclear and Radiochemistry

Washington University, St. Louis, MO

P.hD., 1976 Nuclear and Radiochemistry

Washington University, St. Louis, MO

1976-1977

Postdoctoral Fellow, Nuclear and Radiochemistry

 

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Dr. Knight’s work focuses on using in vivo imaging to follow the behavior of molecules and cells in vivo, and to use functional imaging to learn more about physiology.

Major areas of work include:    Development of radiopharmaceuticals which target vascular receptors. One project is to design and evaluate a new diagnostic imaging test for imaging thrombi and emboli. The approach currently under evaluation is based on a radiolabeled peptide which recognizes a receptor on activated platelets. Another project is to evaluate radiolabeled peptides which bind to receptors in the blood vessels of tumors. The goals of this project are to locate a wide variety of solid tumors by imaging, monitor the effects of anti-angiogenic therapy, and eventually treat the tumors with targeted radiotherapy using a therapeutic radionuclide bound to the same peptide.

Assessment of gastrointestinal motility. Physiologic meals can be prepared containing a radiotracer, so that their transit through the GI tract can be detected and quantified by noninvasive imaging. The effects of drugs and other interventions on the motility of segments of the GI tract can be assessed quantitatively in vivo.

Radiolabeling of drugs to determine their behavior in vivo.   Drugs to be administered to the GI tract have been radiolabeled to assess their duration and extent of coverage of GI mucosa in various diseases such as peptic ulcer and colitis.

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Knight LC. Radiolabeled peptides for tumor imaging. In: Handbook of Radiopharmaceuticals: Radiochemistry and Applications. 643-684. MJ Welch and CS Redvanly, eds. Chichester, John Wiley & Sons Ltd, 2003.

Knight LC. Radiopharmacy aspects of gastrointestinal imaging. In: Nuclear Medicine. Volume II, Chapter 54 (2006). RE Henkin, MA Boles, GL Dillehay, JR Halama, SM Karesh, RH Wagner, AM Zimmer, eds. St. Louis, Mosby Year Book.

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Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

23589143. Sachdeva P, Kantor S, Knight LC, Maurer AH, Fisher RS, Parkman HP, Use of a High Caloric Liquid Meal as an Alternative to a Solid Meal for Gastric Emptying Scintigraphy. Dig Dis Sci :()2013 Apr 16

22293168. Knight LC, Update on gastrointestinal radiopharmaceuticals and dosimetry estimates. Semin Nucl Med 42:2(138-44)2012 Mar

23487404. Knight LC, Romano JE, Krynska B, Faro S, Mohamed FB, Gordon J, Binding and Internalization of Iron Oxide Nanoparticles Targeted to Nuclear Oncoprotein. J Mol Biomark Diagn 1:1()2010

19172383. Pattillo CB, Venegas B, Donelson FJ, Del Valle L, Knight LC, Chong PL, Kiani MF, Radiation-guided targeting of combretastatin encapsulated immunoliposomes to mammary tumors. Pharm Res 26:5(1093-100)2009 May

18378499. Mekapati J, Knight LC, Maurer AH, Fisher RS, Parkman HP, Transsphincteric pH profile at the gastroesophageal junction. Clin Gastroenterol Hepatol 6:6(630-4)2008 Jun

18194154. Cassilly D, Kantor S, Knight LC, Maurer AH, Fisher RS, Semler J, Parkman HP, Gastric emptying of a non-digestible solid: assessment with simultaneous SmartPill pH and pressure capsule, antroduodenal manometry, gastric emptying scintigraphy. Neurogastroenterol Motil 20:4(311-9)2008 Apr

17942806. Knight LC, Kantor S, Doma S, Parkman HP, Maurer AH, Egg labeling methods for gastric emptying scintigraphy are not equivalent in producing a stable solid meal. J Nucl Med 48:11(1897-900)2007 Nov

17921036. Knight LC, Romano JE, Bright LT, Agelan A, Kantor S, Maurer AH, Platelet binding and biodistribution of [99mTc]rBitistatin in animal species and humans. Nucl Med Biol 34:7(855-63)2007 Oct

17499726. Knight LC, Romano JE, Cosenza SC, Iqbal NM, Marcinkiewicz C, Differences in binding of (99m)Tc-disintegrins to integrin alphavbeta3 on tumor and vascular cells. Nucl Med Biol 34:4(371-81)2007 May

15642483. Knight LC, Romano JE, Functional expression of bitistatin, a disintegrin with potential use in molecular imaging of thromboembolic disease. Protein Expr Purif 39:2(307-19)2005 Feb

15366961. Baidoo KE, Knight LC, Lin KS, Gabriel JL, Romano JE, Design and synthesis of a short-chain bitistatin analogue for imaging thrombi and emboli. Bioconjug Chem 15:5(1068-75)2004 Sep-Oct

15366951. McQuade P, Knight LC, Welch MJ, Evaluation of 64Cu- and 125I-radiolabeled bitistatin as potential agents for targeting alpha v beta 3 integrins in tumor angiogenesis. Bioconjug Chem 15:5(988-96)2004 Sep-Oct

15235061. Simonian HP, Maurer AH, Knight LC, Kantor S, Kontos D, Megalooikonomou V, Fisher RS, Parkman HP, Simultaneous assessment of gastric accommodation and emptying: studies with liquid and solid meals. J Nucl Med 45:7(1155-60)2004 Jul

14973420. Knight LC, Non-oncologic applications of radiolabeled peptides in nuclear medicine. Q J Nucl Med 47:4(279-91)2003 Dec

12897712. McQuade P, KnIght LC, Radiopharmaceuticals for targeting the angiogenesis marker alpha(v)beta(3). Q J Nucl Med 47:3(209-20)2003 Sep

12757160. Gonlachanvit S, Hsu CW, Boden GH, Knight LC, Maurer AH, Fisher RS, Parkman HP, Effect of altering gastric emptying on postprandial plasma glucose concentrations following a physiologic meal in type-II diabetic patients. Dig Dis Sci 48:3(488-97)2003 Mar

12141833. Bromer MQ, Kantor SB, Wagner DA, Knight LC, Maurer AH, Parkman HP, Simultaneous measurement of gastric emptying with a simple muffin meal using [13C]octanoate breath test and scintigraphy in normal subjects and patients with dyspeptic symptoms. Dig Dis Sci 47:7(1657-63)2002 Jul

12135014. Miller LS, Szych GA, Kantor SB, Bromer MQ, Knight LC, Maurer AH, Fisher RS, Parkman HP, Treatment of idiopathic gastroparesis with injection of botulinum toxin into the pyloric sphincter muscle. Am J Gastroenterol 97:7(1653-60)2002 Jul

11516697. Knight LC, Radiolabeled peptide ligands for imaging thrombi and emboli. Nucl Med Biol 28:5(515-26)2001 Jul

10855635. Knight LC, Baidoo KE, Romano JE, Gabriel JL, Maurer AH, Imaging pulmonary emboli and deep venous thrombi with 99mTc-bitistatin, a platelet-binding polypeptide from viper venom. J Nucl Med 41:6(1056-64)2000 Jun

10446101. Parkman HP, Trate DM, Knight LC, Brown KL, Maurer AH, Fisher RS, Cholinergic effects on human gastric motility. Gut 45:3(346-54)1999 Sep

9843182. Knight LC, Romano JE, Maurer AH, In vitro platelet binding compared with in vivo thrombus imaging using alpha(IIb)beta3-targeted radioligands. Thromb Haemost 80:5(845-51)1998 Nov

9604828. Maurer AH, Urbain JL, Krevsky B, Knight LC, Revesz G, Brown K, Effects of in vitro versus in vivo red cell labeling on image quality in gastrointestinal bleeding studies. J Nucl Med Technol 26:2(87-90)1998 Jun

9536950. Parkman HP, Urbain JL, Knight LC, Brown KL, Trate DM, Miller MA, Maurer AH, Fisher RS, Effect of gastric acid suppressants on human gastric motility. Gut 42:2(243-50)1998 Feb

9252843. Parkman HP, Miller MA, Trate D, Knight LC, Urbain JL, Maurer AH, Fisher RS, Electrogastrography and gastric emptying scintigraphy are complementary for assessment of dyspepsia. J Clin Gastroenterol 24:4(214-9)1997 Jun

9177512. Knight LC, Parkman HP, Brown KL, Miller MA, Trate DM, Maurer AH, Fisher RS, Delayed gastric emptying and decreased antral contractility in normal premenopausal women compared with men. Am J Gastroenterol 92:6(968-75)1997 Jun

9040203. Fisher RS, Sher DJ, Donahue D, Knight LC, Maurer A, Urbain JL, Krevsky B, Regional differences in gastric acidity and antacid distribution: is a single pH electrode sufficient? Am J Gastroenterol 92:2(263-70)1997 Feb

9009110. Miller MA, Parkman HP, Urbain JL, Brown KL, Donahue DJ, Knight LC, Maurer AH, Fisher RS, Comparison of scintigraphy and lactulose breath hydrogen test for assessment of orocecal transit: lactulose accelerates small bowel transit. Dig Dis Sci 42:1(10-8)1997 Jan

8965152. Maurer AH, Krevsky B, Knight LC, Brown K, Opioid and opioid-like drug effects on whole-gut transit measured by scintigraphy. J Nucl Med 37:5(818-22)1996 May

8772651. Knight LC, Maurer AH, Romano JE, Comparison of iodine-123-disintegrins for imaging thrombi and emboli in a canine model. J Nucl Med 37:3(476-82)1996 Mar

8295000. Knight LC, Radcliffe R, Maurer AH, Rodwell JD, Alvarez VL, Thrombus imaging with technetium-99m synthetic peptides based upon the binding domain of a monoclonal antibody to activated platelets. J Nucl Med 35:2(282-8)1994 Feb

8283521. Chelsky MJ, Rosen SI, Knight LC, Maurer AH, Hanno PM, Ruggieri MR, Bladder permeability in interstitial cystitis is similar to that of normal volunteers: direct measurement by transvesical absorption of 99mtechnetium-diethylenetriaminepentaacetic acid. J Urol 151:2(346-9)1994 Feb

8441057. Knight LC, Scintigraphic methods for detecting vascular thrombus. J Nucl Med 34:3 Suppl(554-61)1993 Mar

1569480. Knight LC, Abrams MJ, Schwartz DA, Hauser MM, Kollman M, Gaul FE, Rauh DA, Maurer AH, Preparation and preliminary evaluation of technetium-99m-labeled fragment E1 for thrombus imaging. J Nucl Med 33:5(710-5)1992 May

1539657. Knight LC, Maurer AH, Wikander R, Krevsky B, Malmud LS, Fisher RS, Effect of ethyl alcohol on motor function in canine stomach. Am J Physiol 262:2 Pt 1(G223-30)1992 Feb

1941157. Maurer AH, Knight LC, Charkes ND, Vitti RA, Krevsky B, Fisher RS, Siegel JA, Comparison of left anterior oblique and geometric mean gastric emptying. J Nucl Med 32:11(2176-80)1991 Nov

2155882. Maurer AH, Knight LC, Siegel JA, Elfenbein IB, Adler LP, Paramagnetic pyrophosphate. Preliminary studies on magnetic resonance contrast enhancement of acute myocardial infarction. Invest Radiol 25:2(153-63)1990 Feb

2136960. Knight LC, Radiopharmaceuticals for thrombus detection. Semin Nucl Med 20:1(52-67)1990 Jan

2582990. Vitti RA, Meyers F, Knight LC, Siegel JA, Malmud LS, Fisher RS, Quantitative distribution of radiolabeled 5-aminosalicylic acid enemas in patients with left-sided ulcerative colitis. Dig Dis Sci 34:11(1792-7)1989 Nov

2781002. Knight LC, Maurer AH, Ammar IA, Epps LA, Dean RT, Pak KY, Berger HJ, Tc-99m antifibrin Fab' fragments for imaging venous thrombi: evaluation in a canine model. Radiology 173:1(163-9)1989 Oct

3177649. Krevsky B, Somers MB, Maurer AH, Malmud LS, Knight LC, Fisher RS, Quantitative measurement of feline colonic transit. Am J Physiol 255:4 Pt 1(G529-34)1988 Oct

3066392. Comerota AJ, Knight LC, Maurer AH, The diagnosis of acute deep venous thrombosis: noninvasive and radioisotopic techniques. Ann Vasc Surg 2:4(406-24)1988 Oct

3062513. Knight LC, Imaging thrombi with radiolabelled fragment E1. Nucl Med Commun 9:10(849-57)1988 Oct

3062512. Knight LC, Imaging thrombi with radiolabelled anti-fibrin monoclonal antibodies. Nucl Med Commun 9:10(823-9)1988 Oct

3351604. Knight LC, Maurer AH, Ammar IA, Shealy DJ, Mattis JA, Evaluation of indium-111-labeled anti-fibrin antibody for imaging vascular thrombi. J Nucl Med 29:4(494-502)1988 Apr

3390351. Knight LC, Maurer AH, Kollmann M, Ammar IA, Fisher RS, Malmud LS, Selenium-75-labeled sucralfate: comparison with other radiolabels and initial clinical studies. Am J Physiol Imaging 3:1(10-8)1988

3343018. Siegel JA, Urbain JL, Adler LP, Charkes ND, Maurer AH, Krevsky B, Knight LC, Fisher RS, Malmud LS, Biphasic nature of gastric emptying. Gut 29:1(85-9)1988 Jan

3254879. Knight LC, Maurer AH, Ammar IA, Siegel JA, Fisher RS, Malmud LS, Use of 111In-labeled alginate to study the pH dependence of alginic acid anti-esophageal reflux barrier. Int J Rad Appl Instrum B 15:5(563-71)1988

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