Seo-Hee Cho, PhD
Assistant Professor, Anatomy and Cell Biology
Assistant Professor, Shriners Hospitals Pediatric Research Center
Telephone: 215-926-9361 (Internal: 7-9361)
Anatomy and Cell Biology
Shriners Hospitals Pediatric Research Center
Mechanisms underlying eye development and retinal degeneration
Our research goal is to understand the molecular and cellular mechanisms underlying normal development and degenerative retinal diseases, and to establish the intervening strategies. Topics we currently study include: (I) Functional analysis of apical polarity gene Pals1 during retinal and lens development, (II) Pathophysiological study of degenerative retinal diseases, LCA (Leber Congenital Amaurosis) and RP (Retinitis Pigmentosa), to understand disease-causing mechanisms. We are particularly interested in polarity defects in retinal progenitor cells, which cause early-onset, photoreceptor degeneration in LCA type 8, (III) Cell-transplantation and gene-based therapies to to restore vision loss in LCA8-like mouse models in animal settings, (IV) Investigating the function of tumor suppressor-oncogene signal transduction pathways, TSC2-mTOR and Hippo-Yap, in eye development. Fundamental questions that we are trying to answer through these studies include, but are not limited to: (1) How is organ size determined? How are cellular contact inhibition signals translated and coordinated to cell proliferation activity in the nucleus? (2) How is cell size determined? How are differential cell sizes achieved and maintained? (3) What are the functions of the apical polarity complex during CNS development? (4) Can visual impairment in degenerative retinal diseases be rescued by gene- or cell-based therapies? Currently, we are using a combination of molecular and cellular methods, high-throughput (microarray), imaging (including confocal and multi-photon) and mouse genetics approaches (including conditional knock-out technology).
Song JY, Park R, Kim JY, Hughes L, Lu L, Kim S, Johnson RL, Cho SH. Dual function of Yap in the regulation of lens progenitor cells and cellular polarity. Dev Biol. 2014 Feb 15;386(2):281-90. doi: 10.1016/j.ydbio.2013.12.037. Epub 2013 Dec 31.
Reith RM, McKenna J, Wu H, Hashmi SS, Cho SH, Dash PK, Gambello MJ. Loss of Tsc2 in Purkinje cells is associated with autistic-like behavior in a mouse model of tuberous sclerosis complex. Neurobiol Dis. 2013 Mar;51:93-103. doi: 10.1016/j.nbd.2012.10.014. Epub 2012 Nov 1.
Cho, S.-H., Kim, J.Y., Simons, D.L., Song, J. Y., Le J. H., Swindell, E. C., Jamrich, M., Wu, S. M., and Kim, S. Genetic ablation of Pals1 in retinal progenitor cells models the retinal pathology of Leber Congenital Amaurosis (2012) Hum. Mol. Genet. 21(12) 2663-2676
Schneider, D.J., Wu, M., Le, T., Cho, S.-H., Brenner, M.B., Blackburn, M.R., and Agarwal, S.K. Cadherin 11 contributes to pulmonary fibrosis: potential role in TGF-b production and epithelial to mesenchymal transition. (2011) The FASEB Journal 26 (2) 503-512.
Kim, S., Lehtinen, M. K., Sessa, A., Zappaterra, M., Cho, S.-H., Gonzalez D., Boggan, B., Austin, C. A., Wijnholds, J., Gambello, M. J., Malicki, J., LaMantia, A. S., Broccoli, V., and Walsh, C. A. The apical complex couples cell fate and cell survival to cerebral cortical development (2010) Neuron 66, 69-84.
Trimarchi, J.*, Cho, S.-H.* and Cepko, C.L. Identification of genes expressed preferentially in the developing peripheral margin of the optic cup (2009) Dev. Dyn. 238, 2327-39. * Equally contributed to this work.
Jadhav, A. P., Cho, S.-H., and Cepko, C. L. Notch activity permits retinal cells to progress through multiple progenitor states and acquire stem cell properties (2006) Proc Natl Acad Sci 103 (50):18998-19003
Cho, S.-H. and Cepko, C. L. Wnt2b/beta-catenin mediated Wnt signaling determines the peripheral fates of the chick eye (2006) Development 133, (16): 3167-3177
Wildner, H., Muller, T., Cho, S.-H., Heppenstall, P., Brohl, D., Lewin, G., Cepko, C., Guillemot, F., and Birchmeier, C.dILA neurons in the dorsal spinal cord are the product of terminal and non-terminal asymmetric progenitor cell divisions, and require Mash1 for their development (2006) Development 133, (11):2105-2113
Blackshaw, S., Harpavat, S., Trimarchi, J., Cai, L., Huang, H., Kuo, W.P., Weber, G., Lee, K., Fraioli, R.E., Cho, S.-H., Yung, R., Asch, E., Ohno-Machado, L., Wong, W.H. and Cepko, C.L. (2004) Genomic analysis of mouse retinal development. PloS Biol 2 (9) E247: 1411-1431.
Das, P., Maduzia, L.L., Wang, H., Finelli, A.L., Cho, S.-H., Smith, M.M., and Padgett, R.W. (1998) The Drosophila gene Medea demonstrates the requirement for different classes of Smads in dpp signaling. Development 125, (8):1519-1528
Padgett, R.W., Cho, S.-H., and Evangelista, C. (1998) Smads are the central component in transforming growth factor b signaling. Pharmacol. Ther. 78 (1): 47-52
Bong, Y.S., Cho, S.-H., Nham, SU and Lee, Y.-I. (1998) Cloning and characterization of cDNAs coding for heavy and light chains of agglutinating monoclonal antibody (HAG12islrh) specific for human red blood cells. Biochem. Biophys. Acta 1380 (2); 156-158
Sohn, M.J., Cho, S.-H., Lee, Y.I., (1995) Rapid and simple purification of human-immunodeficiency-virus type-1 epitope P24 and its use in ELISA. Molecules and Cells 5 (4): 388-392
Sohn, M.J., Cho, S.-H., Jang, W., Chong, Y., Nham, S., and Lee, Y.-I. (1993) Rapid and simple purification of human immunodeficiency virus I epitope gp41. J. Virol. Methods 41 (1): 93-100
Cho, S.-H., Yoon, J., Chang, J., Ahn, B., Lee, C., and Lee, Y.-I. (1993) Genomic typing of hepatitis C virus from Korean patients: implications of genomic variation in the E2/NS1 region. Biochem. Biophys. Res. Commun. 196 (2):780-788
Cho, S.-H., and Kang, C. (1990) DNA sequence-dependent cleavage sites of restriction endonuclease HphI. Mol. Cells 1 (1) 81-86