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Barrie Ashby, PhD

Professor, Pharmacology

Associate Professor, Sol Sherry Thrombosis Research Center

Telephone:  215-707- 4404

Fax:  215-707-9890

Email: barrie.ashby@temple.edu

Department of Pharmacology

Sol Sherry Thrombosis Research Center

Ph.D. Genetics, (1974) University of Leeds, U.K.

 

Postdoctoral Fellow, (1974-78)Department of Biological Chemistry, Washington University School of Medicine, St. Louis, MO.

 

EMBO Fellow (1978-79), Pasteur Institute, Paris, France.

 

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Structure-function studies of prostaglandin E receptors.  Prostaglandins are lipid mediators released from most cells. They are involved in numerous physiological and pathological functions including inflammation, fever, blood pressure regulation and kidney function.  Prostaglandin E2 acts through binding to four subtypes of receptor designated EP1 through EP4.  The laboratory studies the structure-function relationships of the receptors by site-directed mutagenesis and heterologous expression.  Current work is focused on the EP3 subtype, which occurs as several isoforms.  In efforts to understand the significance of the isoforms we have used the yeast-hybrid system to identify proteins that bind to specific isoforms.  Studies are underway to determine the physiological significance of these interactions.    

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Recent Medically Related Publications, Obtained from PubMed (Click on PubMed ID to view abstract)

21261805. Nagy B Jr, Jin J, Ashby B, Reilly MP, Kunapuli SP, Contribution of the P2Y12 receptor-mediated pathway to platelet hyperreactivity in hypercholesterolemia. J Thromb Haemost 9:4(810-9)2011 Apr

20599922. Mao Y, Zhang L, Jin J, Ashby B, Kunapuli SP, Mutational analysis of residues important for ligand interaction with the human P2Y(12) receptor. Eur J Pharmacol 644:1-3(10-6)2010 Oct 10

15304361. Bilson HA, Mitchell DL, Ashby B, Human prostaglandin EP3 receptor isoforms show different agonist-induced internalization patterns. FEBS Lett 572:1-3(271-5)2004 Aug 13

12181444. Ozdener F, Dangelmaier C, Ashby B, Kunapuli SP, Daniel JL, Activation of phospholipase Cgamma2 by tyrosine phosphorylation. Mol Pharmacol 62:3(672-9)2002 Sep

11918350. Sakolsky DJ, Ashby B, Patterns of cyclic AMP formation by coexpressed D1 and D2L dopamine receptors in HEK 293 cells. Receptors Channels 7:6(479-89)2001

11470276. Desai S, Ashby B, Agonist-induced internalization and mitogen-activated protein kinase activation of the human prostaglandin EP4 receptor. FEBS Lett 501:2-3(156-60)2001 Jul 20

11113544. Sakolsky DJ, Ashby B, Determination of D1 and D2 dopamine receptor expression by Ntera-2 cells. Brain Res Mol Brain Res 84:1-2(158-61)2000 Dec 8

11093764. Desai S, April H, Nwaneshiudu C, Ashby B, Comparison of agonist-induced internalization of the human EP2 and EP4 prostaglandin receptors: role of the carboxyl terminus in EP4 receptor sequestration. Mol Pharmacol 58:6(1279-86)2000 Dec

10051157. Bastepe M, Ashby B, Identification of a region of the C-terminal domain involved in short-term desensitization of the prostaglandin EP4 receptor. Br J Pharmacol 126:1(365-71)1999 Jan

9753046. Paul BZ, Ashby B, Sheth SB, Distribution of prostaglandin IP and EP receptor subtypes and isoforms in platelets and human umbilical artery smooth muscle cells. Br J Haematol 102:5(1204-11)1998 Sep

9535991. Zhu J, Luo LY, Mao GF, Ashby B, Liu-Chen LY, Agonist-induced desensitization and down-regulation of the human kappa opioid receptor expressed in Chinese hamster ovary cells. J Pharmacol Exp Ther 285:1(28-36)1998 Apr

9484788. Ashby B, Co-expression of prostaglandin receptors with opposite effects: a model for homeostatic control of autocrine and paracrine signaling. Biochem Pharmacol 55:3(239-46)1998 Feb 1

9442039. Daniel JL, Dangelmaier C, Jin J, Ashby B, Smith JB, Kunapuli SP, Molecular basis for ADP-induced platelet activation. I. Evidence for three distinct ADP receptors on human platelets. J Biol Chem 273:4(2024-9)1998 Jan 23

9154343. Jin J, Mao GF, Ashby B, Constitutive activity of human prostaglandin E receptor EP3 isoforms. Br J Pharmacol 121:2(317-23)1997 May

9203641. Bastepe M, Ashby B, The long cytoplasmic carboxyl terminus of the prostaglandin E2 receptor EP4 subtype is essential for agonist-induced desensitization. Mol Pharmacol 51:2(343-9)1997 Feb

9561143. Ortiz-Vega S, Ashby B, Human prostacyclin receptor: cloning and co-expression with EP3 prostaglandin receptor. Adv Exp Med Biol 433:(235-8)1997

8908618. Mao GF, Jin JG, Bastepe M, Ortiz-Vega S, Ashby B, Prostaglandin E2 both stimulates and inhibits adenyl cyclase on platelets: comparison of effects on cloned EP4 and EP3 prostaglandin receptor subtypes. Prostaglandins 52:3(175-85)1996 Sep

8702478. Akbar GK, Dasari VR, Webb TE, Ayyanathan K, Pillarisetti K, Sandhu AK, Athwal RS, Daniel JL, Ashby B, Barnard EA, Kunapuli SP, Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells. J Biol Chem 271:31(18363-7)1996 Aug 2

8897312. Akbar GK, Dasari VR, Sheth SB, Ashby B, Mills DC, Kunapuli SP, Characterization of P2 purinergic receptors on human erythro leukemia cells. J Recept Signal Transduct Res 16:3-4(209-24)1996 May-Jul

8135729. Kunapuli SP, Fen Mao G, Bastepe M, Liu-Chen LY, Li S, Cheung PP, DeRiel JK, Ashby B, Cloning and expression of a prostaglandin E receptor EP3 subtype from human erythroleukaemia cells. Biochem J 298 ( Pt 2):(263-7)1994 Mar 1

8038705. Ashby B, Interactions among prostaglandin receptors. Receptor 4:1(31-42)1994 Spring

8240268. Li S, Zhu J, Chen C, Chen YW, Deriel JK, Ashby B, Liu-Chen LY, Molecular cloning and expression of a rat kappa opioid receptor. Biochem J 295 ( Pt 3):(629-33)1993 Nov 1

1375452. Smith JB, Dangelmaier C, Selak MA, Ashby B, Daniel J, Cyclic AMP does not inhibit collagen-induced platelet signal transduction. Biochem J 283 ( Pt 3):(889-92)1992 May 1

1378640. Ashby B, Comparison of Iloprost, Cicaprost and prostacyclin effects on cyclic AMP metabolism in intact platelets. Prostaglandins 43:3(255-61)1992 Mar

1722394. Ashby B, Almonor GO, Wernick E, Selak MA, Prostaglandin-concentration-dependent desensitization of adenylate cyclase in human erythroleukaemia (HEL) cells is abolished by pertussis toxin and enhanced by induction by dimethyl sulphoxide. Biochem J 280 ( Pt 3):(801-4)1991 Dec 15

2168700. Williams JA, Ashby B, Daniel JL, Ligands to the platelet fibrinogen receptor glycoprotein IIb-IIIa do not affect agonist-induced second messengers Ca2+ or cyclic AMP. Biochem J 270:1(149-55)1990 Aug 15

1695318. Ashby B, Novel mechanism of heterologous desensitization of adenylate cyclase: prostaglandins bind with different affinities to both stimulatory and inhibitory receptors on platelets. Mol Pharmacol 38:1(46-53)1990 Jul

2110473. Rawala-Sheikh R, Ahmad SS, Ashby B, Walsh PN, Kinetics of coagulation factor X activation by platelet-bound factor IXa. Biochemistry 29:10(2606-11)1990 Mar 13

2155902. Ashby B, Daniel JL, Smith JB, Mechanisms of platelet activation and inhibition. Hematol Oncol Clin North Am 4:1(1-26)1990 Feb

21043980. Ashby B, Prostaglandin Regulation of Cyclic AMP Metabolism in Human Platelets. Platelets 1:1(11-20)1990

2481225. Ashby B, Model of prostaglandin-regulated cyclic AMP metabolism in intact platelets: examination of time-dependent effects on adenylate cyclase and phosphodiesterase activities. Mol Pharmacol 36:6(866-73)1989 Dec

2547839. Ahmad SS, Rawala-Sheikh R, Ashby B, Walsh PN, Platelet receptor-mediated factor X activation by factor IXa. High-affinity factor IXa receptors induced by factor VIII are deficient on platelets in Scott syndrome. J Clin Invest 84:3(824-8)1989 Sep

2848122. Ashby B, Cyclic AMP turnover in response to prostaglandins in intact platelets: evidence for separate stimulatory and inhibitory prostaglandin receptors. Second Messengers Phosphoproteins 12:1(45-57)1988

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