Basic Science Core I: Cell Culture and Neurotropic Viruses, Neuroscience, Proteomics
This Core provides mammalian cell culture and virology services to investigators performing biomedical research on AIDS and the nervous system. Services include the preparation of CNS cell cultures, virus propagation, clinical sample analysis, proteomics, biomarkers, and consultative expertise based in neuroscience. These services promote in uncovering mechanisms involved in the development of nervous system disorders associated with HIV-1 infection.
This section of the Core focuses on the study of HIV-1 infection at the molecular and cellular levels. Services include the purification, characterization, and maintenance of an assortment of central nervous system cells (neurons, astrocytes, oligodendrocytes, microglia, cerebral endothelial cells, progenitor and neural stem cells) and peripheral cells such as macrophages and T-cells. Services also include the isolation, characterization, propagation and maintenance of a variety of HIV-1 clades, HIV-2 and SIV.
For additional information, please contact Dianne Langford, PhD.
We offer expertise to our investigators in a variety of basic neuroscience techniques and procedures, interpretation of results, and in experimental design expertise.
This Core provides state-of-the-art services in proteomics and data analysis, including novel methods for discovering biomarkers, innovative differential expression profiling and bioinformatics, interactomics (protein-protein interaction), and post-translational modifications (PTMs). In addition, this Core assists investigators with identification of discriminative proteins, protein function and pathway analysis, development of prognostic models, and modern methods for biomarker discovery from plasma and cerebral spinal fluid.
For additional information on proteomics, please contact Salim Merali, PhD.
Kamel Khalili, PhD
Basic Science Core I Leader
Department of Neuroscience
Temple University School of Medicine
3500 N. Broad St., 7th Fl.
Philadelphia, PA 19140
T. Dianne Langford, PhD
Salim Merali, PhD