H1 linker histone transitions in the chromatin
of somatic cell
nuclei following SCNT.Cumulus cell nuclei were injected into
MII-arrested ooplasts, or myoblast nuclei were
introduced by electrofusion. The resultant cloned constructs
were fixed at
times indicated. Note that H1FOO assembles on the donor
nuclei within 5 min.
Somatic H1s, in turn, are progressively
lost from the donor nuclei by 60 min post-
cumulus nucleus; cc, condensed cumulus chromosomes; mn,
nucleus; mc, condensed myoblast chromosomes.