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Temple University

 

 

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Week of October 8, 2003

 

 

 

 

Title: The effect of insertion rates on fill length and adaptation of a thermoplasticized gutta-percha technique

Author: Levitan et al

Journal: JOE vol. 29, No.8 August 2003; 505-508

Reviewer: Sang Shin, D.M.D. 

Purpose: To determine the effect of the insertion rate of a thermoplasticized gutta-percha obturation technique (Thermafil Plus) on the quality of the root canal obturation.

Methods and Materials: A calibrated practitioner obturated the tooth in each of  three groups with insertion rates of 18, 6, 3 mm/s (Duration of insertion 1s, 3s, 6s), respectively. The quality of the obturation was assessed by measuring the length of fill and the replication of induced canal irregularities, consisting of dimples and grooves. All measurements were made on a single tooth, with a working length of 18 mm from the canal orifice, embedded in a split mold, with 20 measurements for each insertion rate. Measurements were made under a low-power microscope, with the images recorded on videotape.

Results: The length of fill decreased with decreasing the insertion rate, from a mean overextention of +0.88 mm (SD 1.12 mm) for the 18 mm/s rate to a mean underfill of -0.13 mm (SD 1.03 mm) for the 3 mm/s rate. A trend of decreasing fill with decreasing insertion rate was observed, with the difference between the insertion rates of 18 mm/s and 3 mm/s being statistically significant at p < 0.05 using a single comparison test.  

          Replication of dimples decreased with decreasing the insertion rate from a mean reproduction of 99% for the 18 mm/s rate to a mean reproduction of 90% for the 3 mm/s rate. The replication of grooves also decreased with decreasing the insertion rate from a mean reproduction of 100% for the 18 mm/s rate to a mean reproduction of 88% for the 12 mm/s rate. Statistical analysis of the data for both the dimples and the grooves using a paired comparison post hoc Scheffe test found these differences are significant at p < 0.05.

Conclusion: Although faster insertion led to better replication of irregularities in the extreme apical region, it seemed to come with the loss of control over final length of fill by consistent overextension beyond working length (+0.88mm). If quality of apical replication and length of fill are equally important, one could insert at the faster rate but stop short of working length (0.88mm), allowing the thermoplaticized gutta-percha to flow to the desired working length.

    

 

 

Title: Analysis of continuous-wave obturation using a single-cone and hybrid technique

Author: Guess et. al.

Journal: JOE 2003;29(8):509-512

Reviewer: Jessy Tseng, D.D.S.

Purpose:   To analyze the adaptation of gutta-percha (GP) to prepared root canal walls using two obturation
techniques and determine the influence of the System-B plugger depth on filling adaptation.

Materials and Methods:

  • 56 extracted human mandibular molars were instrumented using Profile NiTi .06 rotary instruments,
  • Teeth were randomly divided into 2 groups.  Group 1 was obturated using the single-cone continuous-wave technique.  Group 2 was obturated with a hybrid technique: lateral condensation followed by a continuous-wave down-pack.  Backfill was not performed.
  • Based on System-B plugger penetration, teeth were divided into three subgroups: (a)2.5-3.5mm; (b)3.5-4.5mm; (c)4.5-7mm from the working length.
  • Roots were horizontally sectioned at 1mm and 3mm coronal to the apical foramen, stained and photographed under the microscope at x16 magnification
  • 4 evaluators scored the adaptation of GP to the prepared canal walls using a three-point scoring system (1=GP adapting to all of the prepared root surface; 2=GP adapting to 2/3 of the surface; 3=GP adapting to <1/3 of the surface)

Results:

  • There is no statistical significance between the two obturation methods at 1mm or 3mm sections in 100% of the samples.
  • Best results were obtained with a plugger depth 3.5-4.5mm from the working length; however, the differences between the three subgroups were not statistically significant.

 

 

Title:  Elimination of candida albicans infection of the radicular dentin by intracanal medications.

Authors:  Siqueira, J.F. et. al.

Journal:  Journal of Endodontics.  29 (8), 2003

Reviewer:  Brett Strong, D.D.S.

Purpose:  To evaluate the effectiveness of four intracanal medications ((Ca(OH)2/Glycerin, Ca(OH)2/0.12% chlorhexidine digluconate, Ca(OH)2/camporated paramonochlorophenol(CMCP)/glycerin, 0.12% chlorhexidine digluconate/zinc oxide) in disinfecting the root dentin of bovine teeth experimentally infected with Candida albicans.

Materials and Methods:

  • Freshly extracted mature bovine incisors used.  Overnight disinfection with 0.5% sodium hypochlorite.   Teeth maintained in phosphate buffer solutions until used.
  • Apical portion and crown removed.  Canal enlarged to a ~2 mm with round tungsten carbide bur.  Root cementum removed.  Root transversely sectioned to create two 4 mm cylindrical sections from each tooth.   Smear layer removed with 3 min 10% citric acid solution exposure.   Dentin cylinders then sterilized.
  • Dentin tubes then incubated for 14 days in 0.1 ml of C. albicans ATCC 10231 culture.
  • 60 specimens (15 each) were then submerged in plates which contained one of the four solutions being tested. Ca(OH)2/ Glycerin, Ca(OH)2/0.12% chlorhexidine digluconate, Ca(OH)2/CMCP/glycerin, 0.12% chlorhexidine digluconate/zinc oxide.  5 specimens were also put in sterile broth.  
  • The plates were aerobically incubated at 37oC for 1 hour, 2 days, or 7 days.  At the designated time intervals 5 specimens from each group were removed and transferred to test tubes containing thioglycolate broth, where they remained incubated for 30 days.   Test tubes were checked daily for growth.  Purity of the culture was verified by plating onto trypticase-soy agar and Sabouraud dextrose agar plates.

Results:

  • Ca(OH)2/ Glycerin = 7 days for predictable disinfection.
  • Ca(OH)2/ 0.12% chlorhexidine digluconate = Never achieved disinfection.
  • Ca(OH)2/ CMCP/glycerin = 1 hour for predictable disinfection.
  • 0.12% chlorhexidine digluconate/zinc oxide  = 1 hour for predictable disinfection.

Discussion:

            As shown in previous studies both C. albicans and E. faecalis  resist elimination when challenged with calcium hydroxide suspended in an inert vehicle (glycerin, saline, water).   Therefore, the antibacterial effects of Ca(OH)2 combined with other antimicrobial medications is being investigated in this and other experiments.  

Prior papers have demonstrated that a Ca(OH)2/chlorhexidine combination is equally as inefficient in eliminating E. Faecalis.   It has been suggested that this is because the chlorhexidine and calcium hydroxide combine to create a solution with a relatively neutral pH; however, no definitive answer has been elucidated.  

In this study both Ca(OH)2/CMCP/glycerin and 0.12% chlorhexidine digluconate/zinc oxide were effective in eliminating C. albicans in less than 1 hour.  However, at this time the authors only recommend a mixture containing  Ca(OH)2 because chlorhexidine has not been  as thoroughly tested with regard to its sequelae when placed as an interappointment medicament.

 

 

 

 

Week of October 22, 2003

 

 

 

Title: Crown-down tip design and shaping

Authors: de Leon, T.P. et. al.

Journal: Journal of Endodontics:29(8)513, August 2003

Reviewer: Vahid Atabakhsh, D.D.S.

Purpose:  To examine the effect of file tip designs on the prevalence of instrument damage, breakage, transportation, and ledge formation during canal shaping by using the crown-down technique.

Materials and Methods: The shaping affects of three types of stainless-steel files that differ in tip shape were studied using curved canals in acrylic blocks.  These were the biconical tip (flex-R ; Union Broach),  conical tip (Mor-Flex ; Union Broach, York, PA), and pyramidal tip (flex-O ; Dentsply Maillefer ).

Curved plastic canals size 25, 0.02 taper were used.   A 25 Flex-R was fitted to the apex in each canal, and radiographed.  The working length (WL) ranged from 16.5 to 18.5 mm.  Each radiograph was magnified 10 times and file path traced on cephalometric paper.  Curvature angle was determined with the Schneider method and demonstrated at 30 to 44 degrees.  All canals were instrumented to 7 mm of apex with #2-6 Gates Glidden, each shaping 2 mm of the coronal canal.  Canals were divided to 3 similar groups, each with 30 specimens.  Two clinicians participated, shaping 15 specimens for each group.  The above three brands of stainless steel (SS) files sizes 60 to 25 were used ( with crown down technique- using 60 first).    Each resin block was mounted and radiographed.  The apical 7 mm were instrumented using one of the 3 files.  Crown-down from #60, clockwise rotation with water as irrigant and recapitulation was done after each file with a #25.  Radiographs were digitized, and NIH image software used to measure the longitudinal cross section in the apical 7 mm.    Data were analyzed by single-factor ANOVA.  

Results: Of the 210 possibilities per instrument group (files #60, 55, 50, 45, 40, 35, 25 = 7 files X 30 canals per group):  

  • Flex-R - file damage incidence was 20, fractured files 0, transportation 4, ledges 0, bypass 0
  • Mor-Flex - file damage incidence was 4, fractured files 0, transportation 69, ledges 0, bypass 0
  • Flex-O- file damage incidence was 59, fractured files 3, transportation 76, ledges 64, bypass 67

The average cross sectional area created by the 3 files in the apical 7mm of the canal space were: 

  • conical -   5.02mm sq. +/-0.34SD 
  • biconical -  5.98mm sq.+/- 0.36SD 
  • pyramidal files -  6.13mm sq. +/-0.47SD 

ANOVA showed significance at P<0.001.  Moreover, canals instrumented with Flex Mor-Flex and Flex-O showed more transportation than Flex-R.   ANOVA showed significance at P<0.001 again.

Discussion/Conclusion:

Both operators concluded that it was more difficult to shape with a pyramidal tipped file as it; required more pressure to advance, threaded the canal rather than shaping it, ledged, required more bypass procedures and was damaged more during instrumentation.   The ridges on the face of a pyramidal shaped tip give it the ability to cause ledging.  Conical shaped tips prevent ledging, but transport and fail to shape the inner wall to the instrument tip.  The biconical tip tends to shape the inner wall to instrument tip.  The incidence of transportation and ledging increases as smaller files go successively deeper and make greater deflection from a straight path.  Thus, crown-down not only frees each instrument from coronal contacts, but also causes the radius of curvature to become less.  The biconical tip reduces the final canal diameter and incidence of transportation relative to the other two tips at all levels (ANOVA, P<0.001).  There were more damaged biconical tipped files which may be due to the fact that the clinicians were unfamiliar with crown-down clockwise rotational instrumentation initially and that unfamiliarity is reflected in the first experimental group, the biconical group.  The biconical tip’s lack of transition angles and formation of lands allows the canal to reorient the tip through curvatures and the file stays centered, thus cutting all sides more evenly.  Thus, ledges are eliminated and transportation is reduced.    There have been other studies that show the same biconical tip design to be superior for curved canals.  (Powell, et al. and Sabala et al.)

 

 

 

Title: Pattern of vertical root fracture: factors affecting stress distribution in the root canal.

Author : Lertchirakarn et al.

Journal : Journal of Endodontics Vol.29 (8), 2003

Reviewer : Sahrip Kim, D.D.S.

Purpose: To compare quantitatively the tensile-stress distribution from various models of simulated canal sections, and to relate stress patterns to fracture patterns observed in teeth subjected to clinical or experimental vertical root fracture.

Material and Methods:

-FEA was used for analysis of stress distribution.

-Developed 6 idealized simple models of dentin and canal

Model I : Cylindrical root and canal
            Model II : Oval root with round canal
           Model III : Cylindrical root with oval canal
           Model IV-VI : Oval root with Oval canal (varying dentin thickness)

1N(Newtons force) applied perpendicularly around the surface of the canal

-Developed models based on fractured roots (digitized cross-section at level of vertical root fracture - VRF)

Model VII : Mandibular Incisor (bucco-lingual fracture)
           Model VIII: Maxillary Premolar (complex fracture due to large post)

6N applied perpendicular to canal surfaces. Distribution of stresses in relation to fracture pattern examined.

-Graphical representations of stress variation in a mid-level cross-section were obtained and maximum tensile stress was computed for each model.

Results:

Simple Dentin models:

  • Tensile stress greatest on the inner (canal) surface and lowest on the outer (root) surface.
  • Stresses radially symmetrical in cylindrical models (decline progressively from inner surface to the outer surface).
  • A change in the cross-sectional shape of the canal or root surfaces resulted in an assymetrical stress distribution.
  • Tensile stress was elevated on the canal or root curvature bucco-lingually.

Fractured tooth roots :

Model VII :

  • Tensile stress oriented bucco-lingual direction
  • Predominantly bucco-lingual fracture, fracture line almost tangential to the proximal canal wall (line does not pass through the middle of canal)
  • Tensile stress distribution mimicked the fracture pattern.

Model VIII :

  • Complex fracture pattern
  • Asymmetrical tensile stress distribution predominantly in a bucco-lingual direction with highest stresses on the root canal surface.
  • The localized high tensile-stress concentrations coincided approximately with the clinical fracture lines.

Discussion:

  • Factors affecting tensile stress distribution are canal shape, root shape, dentin thickness and interactions among these factors.
  • Any localized region of stress concentration would predispose to crack initiation at that location
  • Tensile stresses were highest in inner dentin and concentrated bucco-lingually, but with a subtle inclination toward the distal (concave) root surface.
  • Decreasing proximal thickness increased the tendency for bucco-lingual stress concentration and predispose bucco-lingual fracture.
  • A markedly oval root shape is more susceptible to high stress than a more circular one.
  • A strong similarity between modeled stress and fracture patterns was observed.
  • Irregularity of the root canal in natural teeth increased stress concentration in specific areas and contributed to VRF.

 

 

Title:  Identification of resected root-end dentinal cracks: a comparative study of visual agnification.

Author:  Slaton et. al.

Journal: Journal of Endodontics  29(8), 2003

Reviewer:  Hung Do, D.D.S. 

Purpose:  To evaluate and compare the effectiveness of visual enhancements as aids in correctly identifying artificially created dentinal cracks in resected root ends.

Materials and Methods:

·        50 extracted, permanent maxillary and central incisors were used
·        Teeth were instrumented to a size 50 file
·        The apical 3 mm of each root were resected perpendicular to long axis and examined at X65 magnification with a video microscope to ensure no cracks were present
·        In 27 of the root segments, dentinal cracks were created in the apical dentin with average load of 5.6 kg using a cylindrical wedge in a miniature drill press
·        Teeth with cracks (27) and without (23) were grouped back together, mounted, and randomly selected for inspection.
·        4 examiners viewed the teeth using:

      1.      unaided/corrected vision (group 1)
2.
      loupes at X3.3 magnification (group 2)
3.
      surgical operating microscope at X10 magnification (group 3)
4.
      Orascope at X35 magnification (group 4)

Results:

·        The accuracy of correct identification of cracks using:

      1.      unaided/corrected vision = 39%
2.
      loupes at X3.3 = 45%
3.
      surgical operating microscope at X10 = 53%
4.
      Orascope at X35 = 58 %

·        Three types of cracks were seen:

      1.      canal cracks (both complete and incomplete)
2.
      intradental cracks
3.
      cemental cracks

Discussion:  The study found that with increasing magnification, accuracy gets improved.  Accuracy however was lower than expected.  Two factors that can contribute to this are light reflection and irregularities in the resected root ends.

 

 

 

 

Title:  Finite element analysis and strain-gauge studies of vertical root fractures

Author:  Lertchirakarn, et al.

Journal:  JOE: 29(8)529, Aug 2003

Reviewed by:  J. Lee

Purpose:  Investigate the stress distribution generated in maxillary and mandibular incisors by forces applied with in the canal using finite element analysis (FEA) and to use stain-gauge techniques to validate the root surface strain results obtained from FEA.

Materials and Methods:   One maxillary and mandibular incisor were instrumented and imbedded into epoxy resin blocks; Blocks were crossed sectioned at 1mm x 10 sections.  3D geometry of the root and surrounding tissues created using FEA software.  Two possible loading patterns simulated:  load 1) proximal surfaces of the root canal were subject to point loading, load 2) entire root canal walls were subject to loading.  Maxillary incisor with 170N, mandibular incisor with 60N.  Linear elastic isotropic analysis used on stress distribution in root dentin from canal wall to the outer root surface.  Locations of high stress areas on the root canal surface used to determine potential sites of crack initiation.

Ten maxillary and mandibular incisors were instrumented and obturated to mid-root level by lateral condensation.  Strain gauges placed on buccal/lingual and mesial/distal walls of root surfaces ~apical and middle third of the root and placed in servohydraulic testing machine.  Each tooth subject to increasing force with D11 spreader tip until fracture occurred.

Results:  Distribution of stress by FEA for both maxillary and mandibular incisors with similar results:

  • for both loads 1 and 2 greatest amount of tensile stress along the buccal and lingual canal walls, least amount of tensile stress along the proximal canal walls
  • uniformly low tensile stress along the root surface

Distribution of strain on the outer root surfaces (FEA):  mostly compressive stain on buccal/lingually and tensile strain on proximal.

Strain measurement w/ strain-gauge technique:  mostly compressive strain on the buccal/lingual and tensile stain on proximal.  Proximal stain >> buccal/lingual stain.

Discussion:  Greater distribution of stress on buccal/lingual direction than proximal may be due to asymmetry of canal shape.  Strain measurement by FEA and stain-gauge method with “good” correspondence.  But still no predictable way to suggest patterns of root fractures.

 

 

 

 

 

Week of October 29, 2003

 

 

Title: Repair of perforating internal resorption with mineral trioxide aggregate: A case report.

Author: Hsien  et al.

Journal: Journal of Endodontics Vol.29, No.8 August 2003

Reviewer: Rahul Gupta, D.D.S.

Purpose: To show a case report of perforating internal resorption on a maxillary central incisor and its repair using mineral trioxide aggregate.

Case Report: A 39 year old female with buccal swelling with sinus tract located on the attached gingival of maxillary left central incisor (tooth # 9) for several weeks was referred to the hospital dental clinic. Before coming to the hospital dental clinic the patient had gone to three local clinics for help, where the tooth was opened and debrided.  But the symptoms and signs did not subside as expected, so she was referred to the hospital dental clinic.  In the hospital dental clinic, on performing diagnostic tests the tooth had biting and percussion pain, tenderness on palpation, and mild mobility with normal probing depth.   The patient’s medical history was noncontributory and dental trauma history was denied.  A previous disto-palatal composite resin restoration with extensive secondary caries was noted on the tooth.  Radiographic examination showed an oval radiolucent lesion that was later confirmed as internal resorption in the middle third of the canal.  Access to the canal system was reestablished.  The original canal was negotiable, but the canal could not be completely dried because of continuous exudation and hemorrhage.  Surgical intervention was used.  The apical third was obturated with gutta-percha, and the perforated lesion was repaired with mineral trioxide aggregate(MTA).  A week later the patient returned, then the residual canal space coronal to the MTA was backfilled with a warm gutta-percha system and the tooth was restored with composite resin.  A 2-week follow-up evaluation found that the sinus tract had healed.  The patient was asymptomatic at the 1-year follow-up.

Discussion: Mineral trioxide aggregate has many favorable properties, including good sealing ability, biocompatibility, radiopacity, bacteriostatic activity, and moisture resistance.  There are many studies or clinical case reports of iatrogenic perforation repair with MTA.  This case report demonstrates successful repair of perforating internal resorption with MTA.  

 

 

Title:  Effect of MTAD on enterococcus faecalis contaminated root canals of extracted human teeth

Author: Shabahang, S and Torabinejad, M

Journal: JOE, vol. 29 (9) September 2003

Reviewer:  Michael Moreno, D.M.D.

Purpose:  The purpose of this study was to compare the antimicrobial effect of MTAD with that of NaOCl with and without EDTA.

Materials and Methods:  Eighty-five single rooted human anterior teeth were accessed and sterilized after removing the pulp with a barbed broach.  The teeth were immersed in an inoculum of E. faecalis for four weeks under aerobic conditions at 37 degrees F. After biomechanical instrumentation to size 35 using 1.3% or 5.25% NaOCl as root canal irrigant, the root canal and the external surface of each tooth were exposed to a 5-min application of MTAD, 1.3% NaOCl, 5.25% NaOCl or a 1-min application of EDTA followed by irrigation with 5 ml of 1.3% NaOCl or 5.25% NaOCl. Teeth or dentin shavings were cultured to determine presence or absence of the test bacteria.

Results: None of the 15 samples exposed to MTAD showed any growth after 1 week incubation. 

Conclusion:  Fisher’s exact test showed that the combination of 1.3% NaOCI as a root canal irrigant and MTAD as a final rinse was significantly more effective against E. faecalis than the other regimens.

 

 

 

Title:  Effectiveness of selected materials against enterococcus faecalis:  part 3.  the antibacterial effect of calcium hydroxide and chlorhexidine on enterococcus faecalis.

Author:  Lin et. al.

Journal:  Journal of Endodontics 29(9), 2003

Purpose:  To evaluate the antibacterial effect of calcium hydroxide, chlorhexidine, and a combination of the        on two enterococcus faecalis versus an ampicillin control.

Materials & Methods:  Ten agar plates were swabbed with E. Faecalis, and filter paper points saturated with the following test agent(s) were placed on the plates and incubated for 72 hours:

o       Group 1:  calcium hydroxide powder with sterile water.

o       Group 2:  Pulpdent® (calcium hydroxide paste) with sterile water.

o       Group 3:  Peridex® (0.12% chlorhexidine)

o       Group 4:  calcium hydroxide powder with Peridex®

o       Group 5:  Pulpdent® with Peridex®.

o       Control:   Ampicillin

     Zones of inhibition were measured from periphery of the paper disk after 24 and 72 hours.

Results: No changes in size of inhibition after 24 hours.

    After 72 hours:

o       There was no significant difference between the calcium hydroxide powder and Pulpdent®.

o       Peridex® alone and the combination with either of the calcium hydroxide preparations showed significantly better antibacterial effect than either of the calcium hydroxide preparations alone.

o       There was no significant difference between Peridex® alone and Peridex® combined with either of the calcium hydroxide preparations.

Discussion: Although there was a significant difference between the use of calcium hydroxide and chlorhexidine the authors of the article stated that this might have resulted from the experimental method used.  None-the-less, in this study chlorhexidine showed significantly better antibacterial activity against E. Faecalis than calcium hydroxide, and the addition of calcium hydroxide to chlorhexidine did not enhance the antibacterial effect.

 

 

 

 

Title: The effect of passive ultrasonic activation of 2% chlorhexidine or 5.25% sodium hypochlorite irrigant on residual antimicrobial activity in root canals

Author: Weber, C.D. et al.

Journal:  JOE, 29 (9) 2003, 562-564.

Reviewer: Allyson Byrne, D.M.D.

Purpose: To evaluate the effect of passive ultrasonic activation of 2% chlorhexidine (CHX) and 5.25% NaOCl irrigant on residual antimicrobial activity in root canals.

Materials & Methods: 94 freshly extracted single canal teeth were used.  Instrumentation was completed using the step-down technique.  Between file sizes, teeth were irrigated  w/ 1mm of either 2% chlorhexidine or 5.25 % NaOCl.  Teeth were divided into 5 groups.

  • Group 1- 21 canals irrigated w/ CHX
  • Group 2- 21 canals irrigated w/ CHX plus 1 min of final passive ultrasonic activation.
  • Group 3-21 canals irrigated w/ NaOCl
  • Group 4-21 canals irrigated w/ NaOCl plus 1 min of final passive ultrasonic activation.
  • Group 5-10 canals, negative control, irrigated w/ sterile saline

After instrumentation was completed, canals were further enlarged with a parapost drill to provide a standardized reservoir depth of 8mm, and the apical 3-5mm was covered w/ nail polish. (the majority of apical ramifications are in the apical 3-5mm).  Canals were then rinsed w/ sterile saline (PBS), dried, refilled with PBS and stored.  6hrs after instrumentation, 20 ml of fluid was pipetted from each canal and placed into wells on agar plates, which were inoculated w/ Streptococcus sanguinis.  The plates were incubated and zones of inhibition were measured.  Canals were then rinsed, refilled w/ PBS and stored.  Sampling was repeated 24, 48, 72, 96, 120, 144, and 168 h.

Results:

  • Group 1- 14.7mm zone of inhibition at 6h, 12.9mm at 24h, 11.2mm at 48h, 10.3 at 72h, 10.1mm at 96h, 8.4mm at 120h, 9.1mm at 144h, and 8.5mm at 168h.
  • Group 2-15.6mm at 6h, 13.2mm at 24h, 12.4mm at 48h, 11mm at 72h, 10.6mm at 96h, 9.4mm at 120h, 9.9mm at 144h, 9.8mm at 168h.
  • Group 3- 1.1mm at 6h, 0.2mm at 24h, and no inhibition after 24 hrs!
  • Group 4- 2.2mm at 6h, 0.9mm at 24h, 0.2mm at 48h, and no inhibition after 48h!
  • Group 5- 0.4mm at 6h, 2.2mm at 24h, 1.3mm at 48h, 0mm at 72h, 0.4mm at 96h, and no inhibition after 96 h.

Conclusion:  Residual antimicrobial activity with 2% CHX was statistically significantly superior to 5.25% NaOCl with irrigation alone and with final passive ultrasonic activation.  CHX groups showed antimicrobial activity for as long as 168 h.

 

 

 

 

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