Department of Endodontology

Temple University

 

 

 

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Week of November 3, 2004

 

 

 

 

Title: Evaluation of the incidence of transportation after placement and removal of calcium hydroxide

 

Author: Goldberg et al

 

Journal: JOE Vol. 30 No. 9, September 2004

 

Reviewer: Alex Wang, DDS

 

Purpose: To evaluate the incidence of apical transportation after the placement and removal of Calcium Hydroxide in straight and curved root canals.

 

Materials & Methods: Twenty maxillary central incisors (group A) and 20 mesio-buccal canals from moderately curved roots of mandibular molars (group B) were instrumented and filled with calcium hydroxide using a lentulo spiral. After seven days, calcium hydroxide was removed and post-instrumentation and final radiographs were superimposed to evaluate the incidence of transportation.

 

Results: In group A, no transportation was detected, whereas in group B, 9 of the 20 canals showed apical transportation. This result was statistically significant.

 

Conclusion: During removal, residual pastes of calcium hydroxide could block the apical area of the root, affecting the apical patency in curved canals. This blockage will cause the file to go in a straight direction, transporting the canal anatomy.

 

 

 

 

Title: Accuracy of TMC endo III torque-control motor for nickel-titanium rotary instruments

 

Authors:    Ghassan Yared and Gajanan Kulkarni

 

Journal:    JOE 2004. Vol. 30 (9) pp 644-5.

 

Reviewer: Omar Porras, DMD

 

Purpose:  To determine the actual torque corresponding to four torque settings and evaluate the accuracy of these torque settings on different TCM motors.

 

Material & Methods:

 

Five TMC motors (Sybron Endo) were tested. The actual torque was measured using a size 50 Orifice Shaper (Tulsa Dental) mounted on a 16:1 reduction handpiece that was connected to a digital torque meter memocouple through a chuck that clamped the file. The meter was connected to a computer for measurement recording. Torque 1, 2, 3 and 4 were evaluated at 350 RPM.  The results were analyzed using analysis of variance and post –hoc pairwise comparison.

 

Results:  For each torque settings in all the motors tested, the actual torque settings where significantly higher than the preset and claimed by the manufacturer.

 

Discussion: The usefulness of torque-control motors in reducing instrument fracture is questionable. The lowest torque available in the TMC motors (1.2 Ncm) is higher than the reported torque at fracture for profile .04 No.15 and 25, 0.21 and 0.48 Ncm respectively.

 

 

 

 

 

Title:  Antibacterial efficacy of calcium hydroxide and chlorhexidine gluconate irrigants at 37 ° C and 46 °C

 

Authors: C. Evanov, et al.

 

Journal:    JOE 2004. Vol. 30 (9) pp 653

 

Reviewer: Brian Chang, DDS

 

Purpose: To determine the effectiveness of antibacterial activity of irrigants at body temperatures and an elevated temperature against Enterococcus faecalis.

 

Materials and Methods:

 

  1. Ninty-five bovine mandibular central and lateral incisors were extracted and used and the teeth were sectioned to 5mm cylindrical cross sections with the pulpal lumens standardized to 2.5mm diameters.
  2. The smear layer was removed with 17% EDTA and then autoclaved for 15 min. and the specimens were mounted in individual 22mm diameter tissue wells.
  3. The lumens of the specimens were filled with brain-heart infusion broth containing 1.0 X 107 colony forming units of E. faecalis and the specimens were incubated at 37° for 72 hours.
  4. Two temperatures of 37° C and 46° C were tested for each of three irrigants: saline solution, 10% Ca(OH)2 solution, and .12% Chlorhexidine Gluconate solution.  The negative control consisted of teeth that were inolculated with sterile, uninoculated brain heart infusion broth.
  5. The specimens were filled with their respective test irrigants and then returned to the incubator at 37° C and 46°C for 35 min. The specimens were flushed with sterile saline.
  6. Specimens were weighed, pulverized in liquid nitrogen, and dilutions of the crushed material were plated on brain-heart infusion samples. The plates were incubated at 37° for 24 hours.
  7. The number of bacterial colony forming units was measured.

 

Results:

 

  1. There was no statistical difference in bacterial growth between the two saline groups
  2. The Chlohexidine and Ca(OH)2 group produced significantly less growth than the saline groups.
  3. There was no significant difference in growth between the Chlorhexidine and Ca(OH) 2 groups at the same temperature, but both groups had much lower growth at the higher temperature of 46 ° C.

 

Discussion: The results demonstrated that heat enhances the anti-microbial action of both the 10% Ca(OH) 2 solution and the .12% Chlorhexidine group. These results indicate possible ways of increasing anti-microbial activity in irrigation without resorting to more cytotoxic irrigants.

 

 

 

 

  

 

 

Week of November 24, 2004

 

 

 

Title: An evidence-based analysis of the antibacterial effectiveness of intracanal medicaments

 

Authors:    Amanda Law and Harold Messer

 

Journal:    JOE 2004. Vol. 30 (10) pp 689-94.

 

Reviewer: Omar Porras, DMD

 

Purpose:  To analyze the endodontic literature evaluating the antibacterial effectiveness of intracanal medicaments.

 

Material & Methods: A four-step method of evidence-based analysis was applied.

 

  • Step 1: Search for best evidence using electronic database.
  • Step 2: appraisal and selection of the papers.
  • Step 3: Collection and analysis of the published evidence
  • Step 4: Determine the clinical applicability of the results.

 

The search addressed only human studies. The identified articles were examined following inclusion/exclusion criteria (Table 2). Also the strength of evidence of the papers was analyzed (e.g. RCTs, systematic reviews with meta-analysis, prospective studies).

 

Results:  Only 5 papers met the most significant criteria for inclusion, which was the microbiological sampling post-instrumentation (S2), and post-medication (S3) as well as the pre-instrumentation (S1); all of them evaluating antibacterial effect of Ca(HO)2.

 

The best evidence available was low with all studies characterized as prospective pre-test/post-test experimental trials with no control participants.

 

The combined overall sample size was 164 teeth.  Pre instrumentation cultures (S1) were positive in almost all the canals. After instrumentation (S2) 62% were positive and post medication (S3) 27% of the overall test samples was positive. Of the cultures that were (+) after instrumentation (S2), 45 % culture remained positive at (S3).

No study evaluating other medicaments met the inclusive criteria. However, the combined antibacterial effect of chemo-mechanical preparation and paramonochlorophenol or camphorated phenol medication showed a similar efficacy as IKI with approx. 30% positive cultures at (S3).

 

Discussion: This paper reinforces the evidence that canals cannot by sterilized with chemo-mechanical instrumentation only.  Calcium hydroxide remains the best medicament available. In order to ensure successful outcome it is essential to reduce the bacterial flora to the lowest level possible using Ca(OH) 2 as interappointment dressing for at least 7 days before obturation.

 

 

 

 

Title:  Cold testing through full-coverage restorations

Author: Miller, S.O. et. al.

Journal: JOE, vol. 30 (10): 695 October 2004

Reviewer:  Daniel Bitner, DMD

Purpose:  The purpose of this study was to measure the temperature change occurring at the pulp-dentin junction (PDJ) of extracted premolars restored with PFM or all-porcelain full coverage restorations compared with non restored premolars or those restored with full gold crowns during thermal testing with a ice stick, TFE, or CO2 snow.

Materials and Methods: Ten extracted human premolars were selected as test samples.  The roots were then sectioned 5 mm below the CEJ, and the pulp contents removed.  A thermal conductive medium was placed into the pulp chamber, after which, a thermocouple was secured within the tooth.  Thermal testing was conducted at room temperature.  Thermal testing was first conducted on the intact crowns of the ten samples using a ice stick, TFE using a saturated cotton pellet, and a 3.5 mm diameter pencil shaped piece of CO2  snow.  All testing agents were applied to the middle third of the facial surface for 30 seconds, with measurements of temperature occurring every five seconds.  The thermal test was done three times to each sample.  One sample was tested for 2 minutes to evaluate the prolonged effect of the different thermal tests.  Crown preps were then done on each of the extracted teeth and three coronal restorations for each sample were constructed.  The coronal restorations were a full gold crown, a PFM crown, and an all-porcelain crown.  The same procedure for testing the unprepared samples was then conducted for the prepared samples using the full gold crown, PFM crown, and all-porcelain crown as a restoration.  A thermal conductive medium was placed between the restoration and the tooth during each test.  The all-porcelain crown was then luted into place and tested again.

Results:  Comparison of thermal testing methods found no significant difference between thermal testing methods at 5 seconds for all restoration types.

Discussion:  The results of this study indicates that TFE sprayed onto a #2 cotton pellet provides a more rapid and effective initial temperature reduction than either ice or CO2 snow when testing non restored or crowned teeth.  It was also found that thermal transfer through PFM and all ceramic crowns was similar to thermal transfer through non restored teeth. 

Conclusion: The wide use of PFM and all ceramic restorations and the possibility that many of these restored teeth may require endodontic treatment makes it necessary to have a safe and reliable method to test pulp vitality. 

 


 

 

 

Title: In vivo study on the biocompatibility of newly developed calcium phosphate- based root canal sealers

 

Author: Kim, Jin-Su, Baek, Seung-Ho, and Bae, Kwang-Shik

 

Journal: JOE, Vol. 30, No. 10 (p708-711), October 2004.

 

Reviewer: Brian S. Jeon, DMD

 

Purpose: To compare the biocompatibility of new calcium phosphate-based root canal sealers (CAPSEAL I & II) w/ another commercially available calcium phosphate sealers (Apatite Root Sealer I & II) and a ZOE-based sealer (Pulp Canal Sealer EWT). 

 

Materials & Methods:

 

  • 64 white male Sprague-Dawley rats were assigned to five groups of three animals each for an experimental period of 1, 2, 4, and 12 weeks. 
  • 4 subcutaneous pockets were prepared in each rat by a blunt dissection.
  • A polytetrafluoroethylene tube, 5mm in length and 1.5mm in diameter, containing freshly mixed sealer was placed into each pocket.  Empty tubes were used as control.
  • At the end of each period (1, 2, 4, and 12 weeks), the tubes were removed along w/ the surrounding tissue and fixed.
  • Thin tissue sections were prepared and the tissue responses were graded as being mild (grade 1), moderate (grade 2), and severe (grade 3) according to the criteria suggested by Olsson et al.
  • The results were analyzed by the Kruskal-Wallis test.

 

Results:

 

  • There were no statistically significant differences among the test material groups. 
  • However, there were significant differences among the experimental periods in all sealer groups w/ decreased inflammation observed in later weeks.
  • CAPSEAL I and II groups showed less inflammation than other sealer groups in all experimental periods.

 

Discussion: The early responses of all the groups in this study showed more than moderate inflammation, but the inflammatory reactions decreased w/ time.  Biocompatibility is an important consideration when selecting a material for Endodontic therapy because of direct contact w/ the viral tissue.  However, the currently used root canal filling materials have a tissue-irritating potential (PCS EWT contains eugenol and zinc ions; ARS contains polyacrylic acid).  For this reason, the new calcium phosphate-based sealers will be useful root canal sealers.   

 

 

 

 

 

Title: Identification of resected root-end dentinal cracks: A comparative study of transillumination and dyes.

 

Author: Wright et al

 

Journal: JOE October 2004

 

Reviewer: Rahul Gupta, DDS

 

Introduction: Root-end resection and ultrasonic preparation are commonly performed during apical surgery and could reveal or potentially create cracks in the root dentin. The

dilemma of diagnosing dentinal cracks continues to present a challenge in endodontics.  The definitive diagnosis of a failing nonsurgical endodontic case, in many situations, may be determined only during surgical exposure through direct inspection of the root surface.  Accurate diagnosis of these cracks would provide the clinician with important information to advise the patient better on the long-term prognosis of the endodontic treatment. The purpose of this in vitro study was to compare the effectiveness of transillumination and dyes in identifying root-end dentinal cracks.

Methods and Materials: Fifty maxillary central incisors were de-coronated, and the canals were instrumented to an ISO size 50 at the working length. The apical 3mm of each root was then resected perpendicular to the long axis using a bur in a high-speed handpiece with water spray. Four independent examiners evaluated the root ends at x8 magnification with a surgical operating microscope using

·        transillumination (group 1)

·        sodium fluorescein dye (group 2)

·        caries detect dye (group 3)

·        methylene blue dye (group4)

·        methylene blue plus transillumination (group 5).

The examiners' ability to identify root ends correctly with and without cracks was analyzed by comparing the data with the predetermined standard (cracked and noncracked) using logistic regression analysis.

Results: All techniques used were shown to be more effective than random chance at diagnosing cracks. The areas under the curve (AROC) of the different techniques were as follows: transillumination, 0.81(95 confidence interval); sodium fluorescein, 0.72 (95 confidence interval); caries detector, 0.76 (95 confidence interval); methylene blue, 0.70 (95 confidence interval); and methylene blue plus transillumination, 0.82 (95 confidence interval). Thus, the technique that provided the best AROC, or the best discrimination between cracked and non-cracked resected roots, regardless of rater,was methylene blue plus transillumination.

Discussion: There were no statistically significant differences between transillumination alone and methylene blue plus transillumination. Within the parameters of this study, transilluminating the root end, whether alone or in combination with a dye, appears to be the most accurate way of diagnosing root-end dentinal cracks.

 

 

 

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Created: September 20, 2000 Revised: URL:

 

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