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Week of November 7, 2000

 

 

 
Title: Periapical status of root-filled teeth exposed to the oral environment by loss of restoration or caries

Author: Ricucci, Grondahl & Bergenholtz

Journal: OOO 2000;90:354-9.

Prepared by: Thomas M. Goebel

Purpose: To evaluate the association between coronal restoration integrity and presence of periapical bone lesions in root-filled teeth several years after root canal therapy.

M & M: This is a matched, retrospective cohort study including patients whom received initial root canal therapy and restorative treatment in a private dental clinic over a period of 14 years. A total of 733 patients received endodontic therapy but 210 never returned for clinical or radiographic follow-up. Follow-up data had been collected for 523 patients (71%) for 1 or more years after completion of endodontic treatment. Patients with a follow-up period of less than 3 years were excluded from the study so that existing periapical lesions would have time enough to heal. This limited the sample size to 339 patients with 564 teeth. From this pool, all patients at follow-up with teeth with deep caries or missing restoration were classified as "open group." Patients with intact restorations were classified as the "intact group." Cases were matched based upon pulpal diagnosis, periapical diagnosis, tooth type, observation period, age of patient, quality of root filling & presence or absence of post. One tooth per patient was selected for the study. Teeth were excluded from the study if radiographs were poor or if the apical region was not clearly seen in all radiographs. 55 patients fit the "open" group. All endodontic procedures had been performed using the standardized protocol and 1% NaOCl. Vital teeth were treated in 1 visit while necrotic teeth were treated in 2 visits with an intracanal medicament. Data was collected at follow-up appointments. Coronal aspect of the radiographs was covered up with opaque tape during apical evaluation so that the examiner did not know which group the tooth belonged to. Absence or presence of an osteolytic lesion was determined through the joint assessment between 2 examiners. Comparisons of 55 matched pairs of "open" & "intact" teeth were carried out with McNemar chi-square test.

Results: Mean age for the "open" group was 35.4 yr. and 30.6 for the "intact" group. 95% of teeth were deemed adequately filled. 14 matched pairs for posts occurred. Follow-up radiographs had 14 periapical lesions, 10 "open" teeth & 4 "intact" teeth. 15 teeth (7 "open," 8 "intact") had an unclear periapical condition. 81 teeth were found to have a normal periapical status. Of the matched pairs, 43 of the 55 (78%) had identical periapical conditions. Of the 14 teeth with lesions at follow-up examination, 5 lesions had developed after the completion of treatment (3 "open", 2 "intact"). The remaining lesions (7 "open", 2 "intact") were clearly reduced in size in comparison with the preop condition. The statistical ratio for a lesion to be present in the "open" group was 3.0, which was not statistically significant.

Conclusion: Only 10 of 55 teeth (18%) that had exposure to the oral environment had osteolytic lesions at the last follow-up exam. Of those 10 teeth, 7 had lesions prior to treatment but were clearly reduced in size & 3 had lesions that emerged after therapy. This data suggests that the problem of coronal leakage may not be of such a great clinical importance provided that instrumentation & root fillings are carefully performed.

 

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Week of November 14, 2000

 

 

 
Title: TGF-beta(1) alone and in combination with calcium hydroxide is synergistic to TGF-beta(1) production by osteoblasts in vitro.

Journal: International Endodontic Journal, 33 (5) : 421. September 2000.

Author: A. Jaunberzins, J.L. Gutman, D.E. Witherspoon & R.P. Harper

Prepared by: Greg Dearing, D.M.D.

Purpose: To examine the effects of calcium hydroxide, transforming growth factor-beta, and their co-administration on TGF beta(1)and IL-6 synthesis by early (subculture1) and late (subculture 5) osteoblast cultures.

Methods and Materials: Early and late cultures were established using bone cells harvested from 21 day old fetal rat calvaria. Cell cultures of both early and late osteoblasts were divided into 4 groups: Group #1, control; Group #2, cells challenged with Ca(OH)2; Group #3, cells challenged with TGF-beta1; Group #4, cells challenged with Ca(OH)2 and TGF-beta(1) in combination. TGF-beta(1) and Il-6 levels for all groups were determined using ELISA methodology.

Results: ANOVA and TUKEY HS analyses revealed that osteoblasts of groups 3 and 4 significantly increased (p<0.001) TGF-beta(1) synthesis in both early and late cultures of osteoblasts. IL-6 was not detected in any of the groups considered in this study.

Conclusions: Exogenous TGF-beta(1) has an autocrine effect on cell cultures of osteoblasts. Administration of TGF-beta(1) alone or in combination with Ca(OH)2 increases the synthesis of TGF-beta(1) in osteoblast cultures. Ca(OH)2 and TGF-beta(1) are compatible when placed in a culture of osteoblasts. Ca(OH)2 provides a favorable environment for the anabolic effects of TGF-beta(1).

 

 

 


Title:
Root Canal Anatomy of Maxillary First and Second Permanent Molars

Author: Shalabi et al

Journal: Int End J , 33, 2000

Reviewed by: Brian Frey

Purpose:

  • To study the root canal anatomy of max first and second molar teeth from an Irish population using a clearing technique

M&M:

  • 83 max 1st molars and 40 max 2nd molars were extracted, placed in 10% formal saline, 30 minutes in 5% NaOCl to remove surface debris, crowns removed at CEJ, placed again in 5% NaOCl for 24 hours, dried overnight, India ink injected into canals and drawn through canal by using a central suction system
  • Teeth were then decalcified for 8 days in 10% HCl and then dehydrated in succession with 95% alcohol for 36 hours and 100% alcohol for another 36 hours
  • Teeth were cleared by placing in methyl salicylate , thus making them transparent
  • Teeth examined under dissecting microscope
  • # of roots, canal type, lateral canals, transverse anastomoses, apical foramen position, and apical deltas were collected for data

Results:

  • # of roots – all 1st molars except 2 ( 2 roots= 2.4%) had 3 roots; 2nd molars- 6 had 2 roots (15%)
  • Fusion- fusion of MB and DB roots most common ( 11% for 1st molars, 43% for 2nd molars) - there was a tendency for increased fusion with increased age
  • Most variation in MB root
  • 1st molars MB root – 78% had 2 canals
  • 2nd molars MB root – 58% had 2 canals
  • 1st molar MB root with 2 foramina – 62%
  • 2nd molar MB root with 2 foramina – 50%
  • Lateral canals in 1st and 2nd max molars- most commonly found in palatal roots
  • Lateral canals- 94% in apical third of roots
  • Transverse anastomoses- in MB roots ( 1st molars- 37.8% and 2nd molars- 16.7%) = majority of anastomoses occurred in the middle 1/3 of the root
  • Transverse anastomoses- age was a factor ( lesser probability with older individuals)
  • 2 apical foramen in MB roots- 62% 1st molars and 50% 2nd molars
  • Position of apical foramen- 1st molars MB root ( 22% centrally placed foramina, 36% laterally placed foramina, and 36% had foramen both centrally and laterally)
  • Position of apical foramen – palatal root –more roots with laterally placed foramina than centrally for both 1st and 2nd molars
  • Position of apical foramen- DB root – more centrally placed foramina than lateral
  • Position of apical foramina- age had significance for position of foramina (central position more common in older individuals) only for 1st molars

Conclusion:

  • Significant proportion of maxillary molars exhibited 2 canals in MB root
  • Centrally placed foramina was more common in 1st molars in older groups
  • Transverse anastomoses were more common in younger individuals

 

 


Title: 
Comparison of cold lateral condensation and a warm multiphase gutta-percha technique for obturating curved root canals

Author: Gilhooly, R.M.P., et al

Source: International Endodontic Journal, 33, 415- 420, 2000

Prepared by: Nima Dayani, DDS

Purpose: To evaluate the radiographic quality and sealability of multiphase gutta-percha technique and to compare it with cold lateral condensation of gutta-percha in curved canals.

Introduction: Cold gutta-percha relies heavily on root canal sealer to address the problem of the accessory anatomy. However, there are a number of warm gutta-percha methods available where the movement of filling materials to the accessory canals is possible.

Materials and Methods: A total of 108 human, freshly extracted teeth, with single curved canal, were allocated to two identical groups of 54 teeth. Working length, apical diameter, and angle of curvature were measured (p 416) and then the teeth were distributed between two groups, so that both groups were identical. All the teeth were prepped with NiTi rotary instruments in a crown down fashion to a final apical size of 35, the smear layer was removed with 17% EDTA and NaOCl.

One experimental group was obturated with traditional cold lateral and Extended Working Time Tubliseal (Syborn/Kerr). The other experimental group was obturated using multiphase gutta-percha technique (Alphaseal) and the same sealer.

Multiphase Technique: (FYI)

In this technique, thermo-mechanical compaction has been combined with thermo-plasticized gutta-percha in a technique termed multiphase obturation. A nickel-titanium rotary condenser simultaneously introduces two phases of gutta-percha (alpha and beta) into root canal, which are dispensed form preheated syringes at 70º C.

The Alphaseal system consists of two phases of gutta-percha (Alpha and Beta) in separate pre-filled, disposable syringes and a portable heater. The gutta-percha is warmed in the heater and then extruded onto a size 25/04 taper NiTi rotary condenser for placement into root canal.

"The syringe of beta phase gutta-percha was removed from the heater and a condenser, mounted on a 16:1 gear reduction hand peace, driven by an electric motor was inserted into it through the hub far enough to cover the one half the "grooved" portion. Steady pressure was applied to the syringe plunger to extrude a layer of beta phase gutta-percha onto the condenser. The gutta-percha coated condenser was then inserted into the syringe of alpha phase gutta-percha far enough to cover three quarter of the beta phase material. The instrument was then inserted immediately into the root canal to a depth of 0.5 mm from the working distance, with the condenser rotating for 1S at 4000-5000 rpm. The condenser was then withdrawn while pressure was applied to one side of the canal, with instrument rotating, until the instrument was completely withdrawn from the canal. Gutta-percha at the orifice was then vertically condensed with an amalgam plugger."

Assessment of Obturation: Obturation was assessed based on the following criteria:

  • Extrusion of sealer and gutta-percha
  • Radiographic quality of obturation
  • Apical and coronal dye leakage

Results/ Discussion: Extrusion beyond the apex was significantly more in the multiphase technique. Improved flow of thermo-plasticized gutta-percha also has a disadvantage of extrusion beyond the apex. Also the vertical condensation of the gutta-percha is recommended to counteract the cooling shrinkage, which increases the extrusion, but these teeth had significantly less dye leakage than teeth obturated with cold lateral.

In this study, apical dye penetration was significantly lower for multiphase gutta-percha. This was in agreement with other warm techniques. Additionally, lateral condensation of gutta-percha had a better obturation than the multiphase obturation in terms of radiographic quality from the B-L view; however, this did not correlate with the M-D view.

Conclusion:  Under this study, multiphase obturation had a better sealability, poorer radiographic quality from the bucco-lingual aspect than the lateral condensation.

 

 

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Week of November 21, 2000

 

 


Title:
Computerized infrared thermographic imaging and pulpal blood flow: Part 1. A protocol for thermal imaging of human teeth

Journal : International Endodontic Journal, 33, 442-447, 2000

Author: B.E. Kells, J. G. Kennedy, P.A. Biagioni & P.J. Lamey

Reviewed by: Kimberly Pham, D.M.D.

Purpose: This study was designed to observe the thermographic appearance of teeth and to develop a suitable protocol for imaging teeth in human patients using modern TI equipment.

Materials and MethodA total of 12 unrestored maxillary lateral incisors in 6 healthy patients were imaged under rubber dam after a 20-min equilibration period . A dedicated software package was used to analyze thermographic data. For each tooth, recordings were made from standardized gingival and incisal sites and temperature gradient is established. A sequence of images of both maxillary central incisors in one patient was stored every 30 sec during a 20-mn equilibration period with and without an air-conditioning unit in operation.

Enamel emissivity is defined as the ratio of the radiant power emitted by the surface to that of a blackbody at an identical temperature (A black body is an object which absorbs all radiation.) The emissivity will alter the electromagnetic radiation emitted and consequently the temperature measured at the body surface.

Results & Discussion:  A consistent temperature gradient (mean 1.28°C ) from gingival area to incisal area was recorded. There were no statically significant differences between right and left sides for the gingival site or the incisal site. Without the air-conditioning, there was a mean tooth surface temperature increase of 1.1°C from 0 to 5 mn of the equilibration period and 0.3°C from 15 to 20 mn.

The protocol described is suitable for TI of vital teeth. However TI measured tooth surface temperature only which was extremely sensitive to air currents. A 15 mn acclimatization period under rubber dam was needed to allow stable tooth surface temperature.

 

 


Title:
Efficacy of Quantec rotary instruments for gutta-percha removal.

Author: C.M. Bramante & L.V. Betti

Journal: Internat Endo Journal 33(5):463. September 2000.

Classification: Research Evaluation Article

Key Words: gutta-percha removal, nickel-titanium, Quantec system, root canal retreatment, rotary instruments.

Reviewed by: Paul Slusarz, D.M.D.

Purpose: To evaluate Quantec SC rotary instruments for removal of gutta-percha during retreatment of straight root canals.

Materials and Methods:

  • 30 central incisors were instrumented (stepback technique with K-type files to size 40) and obturated (ZOE sealer and lateral condensation). These teeth were then randomly divided into three even groups.
  • Radiographs for each tooth were taken from the B-L direction and M-D direction.
  • Quantec SC rotary instruments and a 16:1 reduction handpiece were used to remove the gutta-percha and sealer from the canals. A different speed was used in each group (group 1 – 350 rpm, group 2 – 700 rpm, and group 3 – 1500 rpm).
  • The following factors were evaluated: time taken to reach working length, time for gutta percha removal, total time, apically extruded material and number of fractured instruments.
  • Radiographs were taken again in order to evaluate canal wall cleanliness.
  • The teeth were grooved longitudinally, divided, and the walls of each half were evaluated visually for cleanliness. The teeth were then digitized using a scanner and the residual debris was measured.

Results: Group 1 – 350 rpm, Group 2 – 700 rpm, Group 3 – 1500 rpm.

  • Time to reach working length (T1) – group 3 (33.4 sec.), group 2 (1 min. 35 sec.), group 1 (5 min. 57 sec.). The shortest time to reach working length was found in group 3 (1500 rpm).
  • Time for gutta-percha removal (T2) - group 3 (2 min. 51 sec.), group 2 (5 min. 22 sec.), group 1 (7 min. 5 sec.). The shortest time for gutta-percha removal was found in group 3 (1500 rpm).
  • Total time (T1 + T2) group 3 > group 2 > group 1.
  • Amount of apically extruded material all values were similar for the three groups, with group 3 being slightly less than groups 1 and 2.
  • Canal wall cleanliness by radiographic evaluation The difference between the groups were significant only in the middle third of canals. Group 1 had more debris left than groups 2&3.
  • Canal wall cleanliness by visual examination no significant difference between groups.
  • Canal wall cleanliness by computer evaluation no significant difference between groups.
  • Fractured instruments group 1 (6), group 2 (4), and group 3 (1).

Discussion: A speed of 1500 rpm proved to be fastest at allowing instruments to reach the working length and to remove gutta-percha. This may have been because the higher speed plasticized the gutta-percha more quickly. The plasticized gutta-percha would also have presented less resistance and this may explain why only one instrument fractured in the 1500 rpm group. However, despite the higher speed, the amount of apically extruded debris was not greater.

Conclusion: Cleanliness and residual debris were equivalent for each group on straight canals, but the use of 1500 rpm speed was more rapid and fewer instruments fractured.

 

 


Title:
Endodontic Teaching in Philippine Dental Schools

Journal: International Endo. J., 33(5): 427-434, September 2000

Author: Cruz, E.V. et al.

Reviewed by: Lance Isaac

Purpose: The goal of the study was to evaluate the pattern of undergraduate teaching in Philippine dental schools.

Materials and Methods: A questionnaire was prepared, designed to determine details of the teaching of endodontics in permanent teeth. A letter was addressed to the Dean of the College of Dentistry, requesting the questionnaire be completed by the faculty member in charge of endodontic teaching. The respondents were also requested to provide a list of lectures, seminars and practical sessions.

Results:

  • 20 out of 23 dental schools returned completed questionnaires, with no replies to follow-up letters received.
  • Of the 20 responding schools, RCT was the responsibility of the Endodontic Department in only 8, while other schools taught it as a part of Oral Medicine, Restorative Dentistry or Oral Surgery or were not departmentalized at all.
  • Endodontic teaching in all 20 schools took place in the first semester of the third year.
  • Instruction is via formal lectures, with students required to undergo further practice and review in 6 schools.
  • Most topics of endodontics were also covered in other sections of the dental curriculum, with only retreatment remaining the sole responsibility of the endodontic faculty.
  • There were 48 endodontic instructors in the 20 schools, with only 3 having specialist training. For the lab portion of the course, 9 schools had 1 faculty member for 30-45 students.
  • Only 6 schools had course manuals, while most teaching aids were produced by the teachers themselves.
  • 17 schools recommended >1 book, with Grossman’s Endodontic Practice the most popular.
  • Knowledge was assessed via written, practical and oral examination, with some schools requiring reports and group discussions (one school had self-assessment exercises).
  • In the preclinic, 19 schools used extracted human teeth, with one using simulated canals in resin blocks. The amount of time spent working on access cavity preparation, root canal preparation and canal obturation as well as the number of teeth used for each, varied greatly among schools.
  • Working lengths were determined radiographically, with 2 schools also providing apex locators to be used when problems with radiography arise.
  • 19 out of 20 schools used the step-back technique, with one school advocating the step-down/step-back or hybrid technique (other techniques limited to lecture).
  • Most schools used K-type reamers and files.
  • Most schools used NaOCl or H2O2 alone or in combination as the irrigating fluid.
  • Most schools used phenolic compounds (eg. formocresol) or eugenol as the intracanal medicament.
  • Temporary filling materials were mostly IRM, Cavit and Fermin.
  • Lateral condensation was the main obturation technique.
  • The most frequent sealer was slow-setting ZOE.
  • The number of visits required to complete endodontic treatment varied among schools, with most between 2-5 visits, some many as 8.

Discussion / Conclusions: The spread responsibility of endodontic teaching may be seen as beneficial, reinforcing students’ knowledge of a particular topic (though at one school, pulp capping was taught by a periodontist). This points to excess teaching load. The goal should be to meet the requirements of lab courses to have one faculty member for every 25 students. Course manuals are especially indicated in a setting with little individualized instruction. The limited time spent on preclinical exercises also need re-evaluation, in addition to minimum work requirements. There should also be a conscious effort to use endodontic materials and techniques based on current and accepted literature and modalities of treatment.

 

 


Title:
Mandibular first molar with three distal root canals.

Author: Kimura, Y. et al.

Journal: Internt. Endo. Journal 33(5): 468, September 2000

Reviewer: Dr. Mark Wang

Case Report:

  • Patient Information: 31year old female, referred for RCT on #30 because her dentist could not find all root canals; The tooth was asympotomatic, free of any clinical signs including tenderness and percussion.
  • X-ray (PA):presence of periapical radiolucencies
  • Diagnosis: asymptomatic chronic apical periodontitis
  • Endo treatment procedure:
    - Placing a rubber dam, disinfected the crown & surrounding tissue with 5% iodine & 70% alcohol
    - Conventional access, 4 canal orifices were disclosed
    - Orifices were enlarged with a Peeso reamer, distobuccal with 2 separate canals were revealed.
    - Working length of each canal were established with an apex locator, confirmed by x-ray.
    - Cleaning & shaping were completed with files & reamers using conventional method
    - Each canal was irrigated with 5% NaOCl & 3% H2O2 during cleaning & shaping.
    - Ca(OH)2 paste were used as intracanal medicament.
    - All canals were obturated using laterally condensed gutta-percha points & sealer.
  • Follow-up:
    - 3 month asymptomatic with radiographic evidence of progressive periapical healing.
    - 6 month asymtomatic

Discussion & Conclusion:

  • This case describes a mandibular 1st molar with one mesial root & two distal roots. The mesial root & distobuccal root had two canals, and the distolingual root had one.
  • There have been 5 reports on the mandibular 1st molar with three distal canals (Table 1).
  • Only one report described a bilateral case; the other four were unilateral cases, and all were from the right side of the patient.
  • In most right mandibular 1st molars with two distal roots & three distal cannals, the distobuccal root had two canals.

 

 

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Week of November 28, 2000

 

 

Title:  Quality of Apical Seal in Curved Canals Using Three Types of Spreaders

Author: Omar Gani, Ph.D. et al

Source: JOE, 26(10), 581, November 2000

Prepared by: Nima Dayani, DDS

Purpose: To evaluate the influence of spreader shape on the quality of obturation

Introduction: In the present study, authors evaluated the obturation of the apical third of curved canals prepared using step back technique and filled by lateral condensation, with three types spreaders.

Materials and Methods: Forty-five maxillary lateral incisors and maxillary and mandibular canines with apical curvature were selected.
Working length (WL) film taken. WL was ~ 1 mm short of the foramen. Degree of curvature was measured (Schneider method) and classified (Mullaney classification) and the teeth were distributed homogeneously between three groups. The MAF was 35 K. The teeth were step backed to 45 K. Obturation was performed by lateral condensation using three different spreaders.
Group:

  • Conical and Flat Ended (Dentsply-Maillefer) identified A-D with corresponding accessory cone
  • Slightly Conical, pointed end (CC Cord, Zipperer, Munich) sized 15 – 40, with Dentsply-Maillefer cones
  • Similar to Group B but Flat Ended

The gutta percha was the same for groups B and C. Grossman cement was used. The teeth then were decalcified and sectioned at:

  • Section 1 = 2mm
  • Section 2 = 3mm
  • Section 3 = 4mm
  • Section 4 = 5mm

from the apex, and then photographed. The following spaces were then outlined on the photographs: gutta percha mass, sealer mass, foreign bodies, and empty space and then there area was measured.

Results: The defects noted were fundamentally the lack of adaptation of the master cone to the apical third and scarcity of the sealer. In other cases the layer of sealer was the same or more than the master cone. Empty spaces or large masses of sealer were frequent findings at all four levels. Often, they were spaces created by the spreader and left empty. Presence of foreign body or pulp remnants was not infrequent. Radiogrphically, deficiencies were noted that were coincident with the outer wall of the curvature.

Discussion: Allison et al has suggested that better apical adaptation of gutta-percha is achieved by close proximity of the spreader to the apical area. The authors’ study indicated that such proximity of the spreader might not be plausible, as the spreaders tend to follow a straight line and therefore transfer the pressure to dentin rather than gutta-percha. Lack of condensation of gutta-percha was a frequent finding. The author states that the non-adjusted gutta-percha surrounded by a thick layer of sealer was responsible for the apical obturation in sections 1 and 2.

The Author concluded that the presence of pulp remnants were due to inability to use barbed broaches in curved canals as well as reduced apical widening associated with step back technique that does not allow adequate cleaning.

Empty spaces were not infrequent. In group A, no significant difference was noted in sections 1 and 2, between the maser cone area and the sum of all remaining spaces. In group B, adequate condensation was obtained in all sections, (i.e., statistically significant difference was noted between master cone area and all other areas), while in group C, section 1 was not adequately condensed.

Adequacy of condensation:

Section/Group

A

B

C

1

Not good

Good

Not Good

2

Not good

Good

Good

3

Good

Good

Good

4

Good

Good

Good

The author attributed these phenomena to higher flexibility of slightly conical spreaders in groups B and C and pointed tip of the spreaders in group B. Since the master cone in group A was different than those groups B and C, they did comment that it might have influenced the results of group A.

The author recommends use of slightly conical spreaders with pointed tips, rigid accessory points that are considerably thinner than the spreader, as well as coating the cones and the root canal with sealer.

 

 

 


Title: Structure and Chemical Composition of an Experimentally Formed Apical Barrier after the Application of Calcium-Glycerophosphate

Journal: JOE 26(10): 605-609, November 2000

Author: Viloria, I.L. et al.

Reviewed by: Lance Isaac

Purpose: To determine the utility of calcium glycerophosphate (Ca-GP) as an alternative to Ca-BGP and to identify the exact structure and composition of an experimentally formed apical barrier after the application of Ca-GP (equal volume of a and b forms) in the experimental model.

Materials and Methods:

  • In Vitro Experiment: Ca-GP as an alternative to Ca-BGP. Samples were treated, and the resulting specimens embedded in epoxy resin, and examined under TEM and electron diffraction.
  • Animal Experiments: 10 rats were divided into 5 post-op groups (days 0(2h), 1,3,5 and 7). They were anesthetized, and a cylindrical cavity was prepared on each side of the mandible. The roots came from maxillary second molars of rats of the same strain. The roots were packed with Ca-GP paste and implanted in the cavities, covered by glass ionomer cement and the periosteal flap repositioned. After mounting, the samples were examined under SEM fitted with an energy dispersive X-ray microanalysis (EDX) system, as well as under SEM alone.
  • Statistical Analysis: SEM and EDX data subjected to one-way analysis of variance.

Results:

  • In Vitro Experiment: TEM of precipitates revealed an aggregate of needle-like crystals. There was no ultrastructural difference between media containing Ca-GP or Ca-BGP.
  • Animal Experiments: The floor of the cavity was flat and smooth, with no granulation tissue. SEM revealed 3 types of morphological transformation of Ca-GP.

Discussion / Conclusions: Medium supplemented with either Ca-GP or Ca-BGP revealed needle-like crystals consistent with OH-AP (hydroxyapatite). Ca-GP is shown to be able to be used as an alternative to Ca-BGP (which is not readily available), and the a form of Ca-GP may also become a substrate for ALP. There was a significant increase in the average value of the Ca/P molar ratio from days 1 to 3, but it did not increase much from day 3 onward (wound healing after pulp capping is also near complete by day 3 in rat incisors). Overall, this study demonstrates that Ca-GP would be a useful biological medication for artificial barrier formation, especially in cases where teeth have a wide apex.

 

 


Title: Surface tension of root canal irrigants

Journal: JOE Vol. 26, no. 10

Author: Tasman, Fugen and et al.

Prepared by: Gregory Dearing, D.M.D.

Purpose: To evaluate the surface tension values of established and potential endodontic irrigants to which a surface active agent had not been added. Cetrexidine, a surfactant containing 0.2% chlorhexidine gluconate solution was included in the measurements.

Materials and Methods: The solutions studied were:  Distilled water, Ringer’s solution, Sterile physiologic saline, 2.5% NaOCl, 5% NaOCl, 17% EDTA, 3%H2O2 ,Citanest-Octapressin 3%, Ultracaine DS and Cetrexidine. The surface tension was measured using the ring method on a DuNouy tensiometer at standard room temp. Readings were taken in dyne/cm. Seven readings were taken for each solution and averaged.

Results:  Cetrexidine = 32, Citanest = 44, Ultracaine = 47, 2.5% NaOCl = 41, 5%NaOCl = 43, 17%EDTA = 46, Ringer’s solution = 68, H2O2 = 63, Saline solution = 66, Distilled water = 70.

Discussion:  -Ideal irrigant = Antimicrobial, Tissue dissolving, Biocompatible, Good wetability.

                     -Polysorbate 80 and 0.1% fluorochemical surfactant added to traditional irrigants has significantly
                       improved the surface tension properties

                     -With superior properties of irrigants comes the possible need to remove less dentin in root canal                          preparation because of better penetration of tubules

                     -NaOCl and EDTA show favorable surface tension = Good success?

                     -Limitations = Need a standardized surface active agent for comparative testing

 

 


Title: Molecular Epidemiology and Association of Putative Pathogens in Root Canal Infection

Journal: JOE, Vol. 26, No. 10, October 2000

Authors: Jung, Il-Young et al.

Reviewed by: Kimberly Pham, DMD

Purpose:  The purpose of this study is twofold: (1) to investigate the epidemiology of putative pathogens in root canals with apical periodontitis; and (2) to determine the association among the putative pathogens. This work was performed at the molecular level using PCR and dot-blot hybridization.

Materials & Methods:

  • 18 symptomatic (spontaneous pain, tenderness to percussion, presence of sinus tract or swelling, depth of periodontal pocket) and 20 asymptomatic teeth from 36 subjects were studied.
  • PCR and hybridization using rRNA-based oligonucleotide probes.

Results & Discussion:

  • The most frequently found species was Fusobacterium sp. (68%), followed by Peptostreptococcus micros (44.7%) and Porphyromonas gingivalis (26.3%).
  • Sixteen teeth (42.1%) contained one or more species of black pigmented bacteria.
  • B. forsythus and Treponema sp. were detected in 8 and 6 teeth, respectively.
  • The relationships among the selected pathogen are presented with odds ratios. Significant associations were found in the combination of B. forsythus/P. gingivalis (odds ratio 8.333). This relationship is predictable because both bacteria were frequently found in periodontal infection. The association of P. gingivalis and Treponema sp. (odds ratio 27.000) was confirmed as well. Grenier has proved that growth of these bacteria was significantly enhanced in their mixture due growth factors produced by both bacteria.
  • There was no significant association between any bacteria and any symptoms. However, P. nigrescens were detected only in acute cases during the study.

Conclusion:  The combining PCR and hybridization is very useful for detecting bacteria in comparing with culture methods. Most bacteria more frequently than previous studies were detected include fastidious organisms such as
B. forsythus and Treponema sp. The close association between some bacteria that was suggested in previous studies was confirmed as well.

 

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Temple University School of Dentistry. All contents copyright (C) 1996. All rights reserved.
Created: September 20, 2000 Revised: URL:

 

Roy H. Stevens, DDS, MS; Wanda Gordon, D.M.D. - Comments to author:

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