Department of Endodontology

Temple University

 

 

 

 

 

 

 

 

 

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Week of November 5, 2001

 

 


Title:
Cross-reactivity studies of gutta-percha, gutta-balata, and natural rubber latex

Author: Costa G.

Journal: JOE , vol. 27, September 2001

Reviewer: Pranav Vohra, D.M.D.

Purpose: To use radiosorbant test inhibition analysis to examine if raw gutta-percha, raw gutta-balata, or seven clinically used GP products actually release proteins that cross-react with Havea latex (natural latex protein).

Materials &Methods:

  • Using a series of extraction methods, the protein component of the various products ( raw GP, raw gutta-balata, commercial GP, Obtura, and Ultrafill GP) was retrieved.
  • RAST inhibition analysis with I-125 labeled anti-human IgE was carried out for the various samples, to check for cross-reactivity.
  • Powdered latex glove extracts were used for positive control and vinyl glove extracts for negative control.

Results:  There was no cross reactivity between the negative control, gutta-percha, and synthetic trans-polyisopropene.   Both the positive control and gutta-balata extract showed cross reactivity with the havea latex allergen specific human IgE antibody.

Discussion:

  • The potential for cross-reactivity between GP, gutta-balata, and natural latex is due to their isolation from trees of the same botanical family.
  • The study shows that there is no cross reactivity between GP and latex, and this may be due to the denaturing of the plant protein by gasoline treatment used in its preparation, (final preparation includes 20% GP, 56% ZOE filler, 11% Barium Sulfate for opacity, and 3% plasticizers).
  • There is no cross- reactivity seen with synthetic trans-isopropene either, however sometimes manufacturers add gutta-balata to the commercially available gutta-percha and the authors warn of the potential allergic hypersensitive reactions in cases where gutta-balata is added, even in concentration less than 1%.
  • The study shows that the possibility of allergic hypersensitivity reaction from gutta-percha poses no threat to individuals sensitive to latex, however the addition of any amount of gutta-balata to the GP preparation should be discouraged.

 

 

 


Title:
Implantation of bacteria from human pulpal necrosis and translocation from root canals in gnotobiotic mice

Author: Sobrinho, et.al.

Journal: JOE 27 (10):605, Sept. 2001

Prepared by: Greg Dearing, D.M.D.

Purpose: To determine whether microorganisms recovered from infected human root canals were able to survive and translocate*  to a local lymph node when experimentally inoculated into the root canal system of germ free mice.

 *translocation: the passage of viable indigenous bacteria from a given site to the regional lymph nodes and other organs

3 Mechanisms

Rupture of the physical barrier

Microbial ecological unbalance

Deficiency of the host immune defenses

Materials and Methods:

  • The microorganisms isolated from 2 human patients with pulpal necrosis were inoculated in Five groups of experimental animals (gnotobiotic mice = animal models).
  • Group 1 Gemella morbillorum.
  • Group 2 Bifidobacterium adolescentis, Fusobacterium nucleatum, Clostridium butyricum.
  • Group 3 Bifidobacterium adolescentis
  • Group 4 Fusobacterium nucleatum
  • Group 5 Clostridium butyricum
  • Groups of mice between 2 and 6 were sacrificed on days 2, 3, 6, and 10 days post inoculation and the root canals cultured along with removal of submandibular lymph node.

Results:

  • G. morbillorum showed the highest frequency of colonization and translocation to the draining lymph node
  • In group 2 only F. nucleatum and C. butyricum colonized and translocated when inoculated in tri-association
  • When the bacteria from group 2 were inoculated in mono-infection all 3 species colonized the root canal of germ free mice and translocated to the draining lymph node, but with different frequencies.

Conclusions:  Selective mechanisms occur in which some bacterial species are fit to survive, multiply, and translocate in the germ free mouse model.

 

 


Title: Radiographic Evaluation of Periradicular Repair after Endodontic Treatment of Dog’s Teeth with Induced Periradicular Periodontitis

Author: Grecca, Fabiana Soares et. al.

Journal: Journal of Endodontics, 27(10):610, September 2001

Reviewer: Donna Salin, D.M.D.

Purpose: To evaluate the effects of two calcium hydroxide pastes and two sealers used in endodontic treatment, on the periradicular repair of teeth, via radiographic evaluation.

Materials and Methods:

  • Eighty-four root canals of premolars from six dogs were left open for 7 days to allow microbial contamination, and then sealed and followed for 45 days until periradicular periodontitis developed.
  • The root canals were then treated endodontically using 5.25% sodium hypochlorite as the irrigating solution.
  • After instrumentation, all root canals were filled with a calcium hydroxide-based dressing Calen PMCC or Calasept, that was left in place for 30 days; the root canals were then filled with gutta-percha cones and a root canal sealer, Sealapex or AH Plus, using cold lateral condensation.
  • The groups were broken down as follows:
    • Group I - Calen PMCC + Sealapex
    • Group II - Calasept + Sealapex
    • Group III - Calen PMCC + AH Plus
    • Group IV - Calasept + AH Plus
  • Periapical radiographs of the teeth were made after root canal filling and after 90, 180, 270, and 360 days and radiographic images were digitalized by scanning in order to make the periradicular radiolucencies more visible ; the Mocha program (version 2.1, Jardal Scientific, Atlanta, GA) was used to measure the periapical lesions.

Results: The periapical lesions al all groups were statistically similar at the beginning of treatment. To evaluate the decrease in the periapical radiolucent areas the final values were compared with initial values for each experimental group. At 360 days, the lesions of groups I to III had a statistically similar reduction in size, whereas group IV had a smaller reduction in lesion size (p < 0.05).

Discussion:

Calcium hydroxide associated with PMCC (composition: 2.5 calcium hydroxide, 1 g zinc oxide p.a., 0.05 g colophony, 2 ml polyethylene glycol 400, and 0.04 g camphorated p-monochlorophenol) allows a controlled liberation of calcium and hydroxyl ions. This material forms calcium p-chlorophenolate, which maintains a high pH and allows the liberation of calcium and hydroxyl ions for a longer period, which may be the reason for its good results independent of the sealer used. Aqueous vehicles, such as Calasept paste, have a rapid ionic dissociation of the ions and consequently short-term action, which may explain the poorer results seen with Calasept paste (when used with the AH Plus sealer in Group IV).

The antibacterial action of Sealapex is related to the calcium and hydroxyl ionization and also to the maintenance of the high pH in tissues, even after setting. The antibacterial action of Sealapex and its biocompatibility can explain the good results obtained by the sealer when associated with both of the calcium hydroxide pastes.

 

 

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Week of November 12, 2001

 

 


Title:  Susceptibilities of enterococcus faecalis biofilms to some antimicrobial medications

Journal: JOE: 27(10): 616-619, September 2001

Author: Lima, KC et al.

Prepared by: Lance Isaac, D.M.D.

Purpose: The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms.

Materials and Methods: Biofilms of E. faecalis were induced on cellulose nitrate membrane filters. Nine membranes were used for each Mitis salivarius agar plate, which were then incubated for either 1d or 3d at 37° C in an aerobic atmosphere. The antimicrobial medications tested in gel form were:

  1. 2% gluconate chlorhexidine, 2% natrozole, in distilled water
  2. 2% gluconate chlorhexidine, 1.25% lauryl-diethylene-glycol-ether-sodium sulfate, 2% natrozole, in distilled water
  3. 2% clindamycin, 2% natrozole, in distilled water
  4. 2% clindamycin, 1.25% lauryl-diethylene-glycol-ether-sodium sulfate, 2% natrozole, in distilled water
  5. 2% gluconate chlorhexidine, 15% zinc oxide, 1.25% lauryl-diethylene-glycol-ether-sodium sulfate, 2% natrozole, in distilled water
  6. 2% clindamycin, 10% metronidazole, 1.25% lauryl-diethylene-glycol-ether-sodium sulfate, 2% natrozole, in distilled water

control: 2% natrozole, 1.25% lauryl-diethylene-glycol-ether-sodium sulfate in distilled water

In the biofilm susceptibility assay, 1mL of each of these medications were used to cover each biofilm-containing membrane. Uncovered biofilm membranes served as controls for adhered/lost cells (=microorganisms). Six biofilm membranes were used for each group. Treated biofilms were aseptically transferred to vials containing a neutralizing agent in saline solution and vortexed for 1min. Suspensions were 10-fold diluted, seeded onto Mitis salivarius agar plates and the colony-forming units (CFU) counted after 48h of aerobic incubation at 37° C. Only a, b and e were tested against the 3d biofilms.

Results/Discussion: Among 1d biofilms, a, b, e and f significantly reduced the number of viable bacterial cells in the biofilms. However, only chlorhexidine-containing medications (a, b and e) were able to completely eliminate most of the biofilms. Set d. and the control did not affect the biofilm, while c allowed bacterial overgrowth. Because of the results of the 1d biofilm, only a, b, and e were tested against 3d biofilms, and all 3 were found to eliminate or at least reduce the bacterial load (no significant differences among them).

 

 

 


Title:  The continuous locking suture technique (CLST)

Journal:  JOE 2001; 27(10): 624-6

Author: Kleier DJ

Reviewer: Kimberly Pham, DMD

Purpose:  To describe how the endodontic surgeon and surgical assistant work as a team to effectively and efficiently use this technique.

Technique:

  • This suture is recommended for closing Ochsenbein-Luebke flaps in anterior and posterior regions.
  • Place an interrupted suture at one end of the flap (penetrating the tissue from the most mobile tissue to the least mobile tissue, at 2-3 mm from the incision line, cut the short end, leave the long end connected to suture needle)
  • The assistant holds the long end of the suture to from a loop
  • Passing the needle through the resulting loop and pulling the suture tight against the adjacent tissue forms the locks.
  • Locking loops are placed 2-3 mm apart until the distal end of the flap is reached.
  • The last loop forms an end that is used to tight the last suture.
  • Sutures are removed by cutting the loops close to the tissue on the flap side.

Advantages:

  • Fewer knobs, less tissue irritation
  • Controlled tension on flap margin
  • Easier to keep clean
  • Sutures are easily removed

Disadvantages:  If any sutures pull through the tissue, the integrity of the suture line could become compromised. Inform patient this possibility

Discussion:  CLST can be used to close

  • Soft tissue in edentulous areas
  • Periodontal flaps such as modified Widman flaps (full thickness flap including gingival margin, used in endo surgery sometimes)
  • Ochsenbein-Luebke flaps
  • Silk suture material (4-0) is easy to tie but support bacterial growth. Chlorhexidine gluconate mouthwash is used to reduce bacterial populations on sutures and wound margin

Sutures should be kept in place only long enough to ensure initial healing by primary intension. (For intrasulcular incision, junctional epithelium repopulate after 48 hours)
To describe how the endodontic surgeon and surgical assistant work as a team to effectively and efficiently use this technique.

 

 

 

Title: A comparison of mesial molar root canal preparations using two engine-driven instruments and the balanced-force technique

Author: Imura et al.

Journal: JOE: 27(10): 627-631, October 2001.

Submitted by: Paul Slusarz, D.M.D.

Purpose: To compare the effects of two engine-driven, nickel-titanium instrument systems with hand files in the final shape of slight and moderately curved canals.

Materials & Methods:

  • A total of 72 mesial roots of extracted human mandibular molars were divided into three groups: ProFile 0.04 taper, Pow-R rotary systems, and Flex-R hand-filing technique. Each test group examined roots having both small curvatures (11 to 24 °) and moderate curvatures (25 to 39 °).
  • The roots were mounted and cross-sectioned at two different horizontal levels (3mm and 5mm from the apex). The coronal, middle and apical segments of each root were mounted on a special jig made of two aluminum pieces in order to maintain the exact configuration of each root.
  • In group 1, canals were instrumented using RBS (Rapid Body Shaper) followed by Pow-R rotary files.
  • In group 2, canals were instrumented using Profile 0.04 files in a crown-down technique.
  • In group 3, canals were instrumented using Flex-R files in a balanced force technique, then Gates-Glidden drills were used to flare the coronal portion.
  • Pre-instrumented and post-instrumented cross-sectional roots were imaged, recorded and computer analyzed.

Results:

  • In the middle third in almost all groups, there was a tendency of cutting more toward the mesial side with only one exception: Pow-R cuts more to the distal side.
  • At the apical third, Flex-R and Profile transported to the mesial side when the curvature increased.
  • When the three techniques were compared analyzing each side and considering the two groups of curvature, at the middle third in moderately curved canal group, Flex-R cut statistically more than the Pow-R toward the lingual side. The other comparisons showed no statistically significant differences.
  • When the techniques were compared in relation with the degree of curvature, in the apical third, Profile 0.04 cut statistically more toward the mesial side in the moderately curved canal group than in the slightly curved canal group. The other comparisons showed no statistically significant differences.
  • Canal preparation time was shorter with hand instrumentation in a few instances.

Discussion:

This study attempted to evaluate several aspects of two new endodontic rotary instruments and one hand instrumentation to prepare canals with slight and moderate curvatures. In this study, on the average, all techniques instrumented all four walls analyzed, leaving just a few specimens with untouched walls.

 

 

 

Title:   Removal of fractured posts using ultrasonic vibration: an in vivo study.

Author: Smith BJ, et al.

Journal: JOE 2001; 27(10): 632

Reviewer:  Mark Wang, DMD, MS.

Purpose: To determine the effectiveness of ultrasonic vibration in the removal of fractured posts from teeth under true clinical conditions.

Material & Methods:

  • 30 patients (16 men &14 women, mean age of 51 yr) with a fractured post were studied.
  • The coronal part of the post were exposed and cut with an 2.0 mm gutter around the post by a fine diamond bur.
  • The tip of a piezoelectric ultrasonic scaler was applied to the side of the post fragment at full power with H2O irrigation to cool the tip.
  • The ultrasonic vibration was applied for two 15 sec periods followed by 30 sec periods.
  • After each period the coronal part of the tooth was dried using compressed air and the post fragment was tested for looseness using a dental explorer.
  • After dislodgement the loose fragment was removed with fine forceps, or when too deep for forceps, a Masserann trepan (Micro Mega).
  • Cotton wool was placed in the post hole, access sealed with a ZOE, and the patient was referred back to the referring dentist.

Results & Conclusion:

  • Ultrasonic vibration time was significantly correlated with the length of a cemented post fragment rather than its diameter.
  • Ultrasonic vibration time (mean 2.05 min) was 25% of that found in studies of post removal in extracted teeth.
  • In the patients who performed the study group, mean post length was <50% of root length.
  • Ultrasonic vibration is an effective method for removal of fractured posts.

 

 

 

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Week of November 19, 2001

 

 

 

Title: Influence of rotational speed, torque and operator’s proficiency on ProFile failures

Author: G.M. Yared, F.E. Bou Dagher, & P. Machtou

Journal: International Endodontic Journal: 34: 47-53, 2001

Reviewer: Donna Salin, D.M.D.

Purpose: To evaluate the influence of rotational speed, torque, and operator experience with a specific Ni-Ti rotary instrumentation technique on the incidence of locking, deformation and separation of instruments.

Materials &Methods:

  • Profile Ni-Ti rotary instruments with a 6% taper were used in a crown-down technique.
  • 1st group (300 canals): speeds of 150, 250, and 350 rpm were used (with a fixed torque of 20 Ncm).
  • 2nd group (300 canals): torque set at 20, 30, and 55 Ncm (with a fixed speed of 150 rpm).
  • 3rd group (300 canals): three operators with varying experience were used (with a fixed torque of 20 Ncm and speed of 150 rpm).
  • Each subgroup included the use of 10 sets of Profile instruments and 100 canals of extracted human molars.
  • 2.5% NaOCl was used as an irrigant.
  • The number of locked, deformed, and separated instruments for the different groups, and within each part of study was analyzed.

Results:

In the 1st group only 1 instrument was deformed in the 150 rpm group and no instruments separated or locked. In the 250 rpm group instrument separation did not occur. A high incidence of locking, deformation, and separation was noted in the 350 rpm group.

In general, instrument sized 30-15 locked, deformed, and separated. Overall, there was a trend toward a higher incidence of instrument deformation and separation in smaller instruments.

In the 2nd group, neither separation nor deformation and locking occurred during the use of the ProFile instruments at any of the different torque values.

In the 3rd group, analysis demonstrated that significantly more instruments separated with the least experienced operator. Instrument locking, deformation, and separation did not occur with the most experienced operator.

Discussion:

Deformation and separation occurred during the final passage of PRI in the last (tenth) canal in each subgroup of the 1st group. That may be due to the fact that the operator, being aware it was the final passage, not adhere strictly to the guidelines and applied excessive apical pressure on the instrument.

In the 2nd group, neither deformation nor separation occurred. This was probably due to the low speed of 150 rpm as well as the strict adherence to the clinical guidelines and the minimal load exerted on the instruments.

The inexperience operator in the 3rd group probably exerted excess apical pressure on the PRI and/or used them for too long in the canal. Consequently, the PRI locked into the canal and were subjected to high level torque.

Overall, training in the use of the PRI technique, a speed of 150 rpm, and adherence to specific guidelines were all crucial in avoiding instrument separation and reducing the incidence of instrument locking and deformation.

 

 

 


Title: A study of endodontic treatment carried out in dental practice within the UK

Author: Jenkins, SM., Hayes, SJ. Dummer, PMH.

Journal: International Endo Journal, 34, 16-22, Jan. 2001

Key Words: dental practice, endodontics, UK.

Reviewed by: M. Pallante, D.M.D.

Purpose: To gather both quantitative and qualitative information on the nature of root canal treatment carried out by a group of dentists working within the United Kingdom.

Materials and Methods:

720 graduates from the Cardiff Wales Dental School were sent a questionnaire. The first part of the questionnaire requested basic information (age, year of qualification, type of practice, and average number of root fillings completed per month. The second part of the questionnaire consisted of 15 questions, most of which included a list of possible answers. The questions were designed so as not to be leading. The questions were basically focused on operator technique and protocol. The answers were categorized in 6 groups corresponding to the percentage of time that each step was followed. The results were tabulated and processed and was then categorized first according to age and second according to the average number of root fillings completed per month.

Results:  Two hundred ninety-nine questionnaires containing useful information were returned.

  • Mean age of respondents was 34.5 yrs. (r=22-56).
  • Mean number of root fillings per month was 12.8 (r=1-60). 
  • No relationship between the number of fillings and age of the operator.
  • 19% of respondents reported using a rubber dam routinely.
  • 89% of respondents took a radiograph to determine working length while a small number relied on tactile sensation.
  • A variety of different methods were utilized for instrumentation (reamers, K-files, FlexoFiles, Hedstroms, etc.) Step-back was the instrumentation method most commonly performed.
  • Most used local anesthetic as an irrigant, while sodium hypochlorite was second most prevalent.
  • As far as filling canals, most used gutta percha and lateral condensation for anterior teeth and posterior teeth. Interestingly, a significant amount (16%) reported using full length silver points in posterior teeth, while a negligible percentage (<.3%) practiced this with anterior teeth.
  • Finally, 75% of practitioners reported taking a post operative radiograph to verify the position of the root filling after cementation.

Discussion:  The authors are careful to say that this data cannot be considered representative of the general dental population in the UK since it was restricted to graduates of one particular dental school. However, this data is advantageous in relation to the teaching of endodontics. It is evident from this survey that a large proportion of dentists use techniques that are not currently favored by expert opinion.

 

 

 

Title: A survey of interfacial forces used during endosonic instrumentation of root canals

Journal: International Endodontic Journal, 34, 54-62, 2001

Author: Regan et al.

Reviewed by: Andy Schoelch, DDS

Purpose: To examine and measure the pattern of interfacial forces acting between root canal dentin and ultrasonic files during endosonic instrumenatation using K-files sizes 15, 20, and 25.

Materials and Methods:  Single rooted teeth were mounted on a cantilevered aluminum beam to which two pairs of single element strain gauges were joined in a half bridge configuration mounted at right angles to each other. The strain gauges were connected to an analogue-to-digital converter fitted in a microcomputer via a conditioning amplifier. This enabled strains to be recorded as a function of interfacial forces over a period of time. Twenty operators instrumented root canals using sizes 15, 20, and 25 ultrasonically energized K-type files.

The lateral forces generated were calculated.

Results:

  • Overall range of forces = 0 –334 g
  • Mean forces range for all three files = 18 – 149 g
  • Consistency in relative magnitude for each operator
  • Interfacial forces tended to increase with file size, these differences were statistically significant
  • Higher forces may have been a function of the increased rigidity of the files, allowing greater transfer of the applied load to the canal wall

Conclusion:  The range of forces measured is broader than previously reported and may have a bearing on uncontrolled dentin removal, even during ultrasonically activated irrigation.

 

 

 

 

Title: Aetiology of root canal treatment failure: why well-treated teeth can fail

Author: Siqueira, J.F.

Journal: International Endodontic Journal   34(1):1-10, 2001

Reviewed by: Alison Morrison, D.M.D.

Purpose: To discuss the aetiology of the failure of root canal treatment and to discuss the indications for the treatment of endodontic failures.

Literature review: Endodontic therapy usually fails when the treatment is not carried out to the highest standards; however, there are times when high quality root canals result in failure. Root canal treatment commonly fails due to secondary intraradicular infection, extraradicular infection or for other nonmicrobial reasons. Other Intrinsic and extrinsic nonmicrobial factors have also been discussed as reasons for endodontic treatment failure.

Conclusion: The literature suggests that persistent intraradicular, secondary infections, and in some instances extraradicular infections are the major causes of failure of both poorly treated and well-treated root canals.

Seltzer et al.1963, Sjogren 1996 and Engstrom et al. 1964 - Root canal treatment fails when treatment falls short of acceptable standards.

Nair et al. 1990 and Lin et al 1992 -  The major factors associated with endodontic failure are the persistence of microbial infection in the root canal system and or the periradicular area.

Reasons for failure:

  1. Intraradicular infections

Kakehashi et al. - Microorganisms colonizing the root canal system play an essential role in the pathogenesis of periradicular lesions.

Moller et al. 1981 - Demonstrated in monkeys that only devitalized pulps that were infected induced periradicular lesions.

Sundquivst 1976 - Confirmed the important role of bacteria in periradicular lesions in a study using human teeth.

Bystrom et al. 1987, and Sjogren et al. 1997 - Showed that if microorganisms persist in the root canal at the time of root filling of if they penetrate into the canal after filling, there is a higher risk that the treatment will fail.

Lin et al 1991 and Siqueira et al. 1996 - Demonstrated that part of the root canal space often remains untouched during chemo-mechanic preparation, regardless of technique and instruments employed.

Atlas 1997 - Under conditions of phosphate starvation triggered by low concentrations of organic phosphates, cells turn on genes for utilization of organic phosphate compounds and for the scavenging of trace amounts of inorganic phosphate.

Untreated teeth usually are composed of mixed infections ( predominately gram – anaerobic rods) and failed cases are composed of only one or a few species ( usually gram +).

Jett et al. 1994, Siqueira & Uzeda 1996 and Siqueira &Lopes 1999 - E faecalis strains have been demonstrated to be extremely resistant to several medicaments, including calcium hydroxide.

Molander et al. 1998 - When E. faecalis is established in the root canal, its eradication by conventional means may be extremely difficult.

  1. Extraradicular infections

Sundqvist & Reuterving 1980, Nair 1984, Happonen 1986, Sjogren et al. 1988 and Sakellariou 1996 - It is currently recognized that some oral organisms, such as Actinomyces spp. and Propionebacterium propionicum may be implicated in extraradicular infections.

Costerton et al. 1987 and Siqueira & Lopes 1998 - A biofilm can be defined as a microbial population attached to an organic or inorganic substrate, surrounded by microbial extracellular products, which form an intermicrobial matrix.

Costerton et al. 1987, 1994 and Gilbert et al. 1997 - Organized in biofilms, microorganisms show higher resistance to both antimicrobial agents and host defense mechanisms when compared with planktonic cells.

Tronstad et al. 1990 - Reported the occurrence of bacterial biofilms adjacent to the apical foramen and bacterial colonies located inside periradicular granulomas.

  1. Microbial involvement in special situations

Strindberg 1956, Engstrom et al. 1964 - Success rate of the root canal treatment is decreased in cases of overfilling. Conversely, it has been reported that the apical extent of the root canal filling has no correlation with treatment failure (Lin et al. 1992).

Barbosa et al. 1993, Spangberg 1998 and Lopes & Siqueira 1999 -  Most of the materials used in root canal obturation are either biocompatible or show cytotoxicity only prior to setting.

Yusuf 1982 - The presence of infected dentine or cementum chips in the periradicular lesion has been associated with impaired healing.

  1. Coronal Sealing

Saunders and Saunders 1994 - It has been found that coronal leakage may be an important cause of failure of endodontic treatment.

Siqueira et al. 1999 - Recontamination of the root canal system by coronal leakage will occur through: sealer dissolution by saliva, percolation of saliva in the interface between sealer and root canal walls and /or between sealer and gutta-percha.
Coronal exposure of the root canal obturation to saliva for a relatively short period of time (30 days or more) might be considered an indication for retreatment.

  1. Nonmicrobial Factors

 

Nair et al. 1998 - It has been demonstrated that cholesterol crystals can be an etiologic factor in nonresolving chronic inflammation.

Although it has been revealed that the majority of periradicular cysts heal after conventional root canal therapy (Morse et al. 1975), it has been suggested that true cysts, which contain cavities completely enclosed by epithelial lining, do not (Nair et al. 1993).

Treatment of Endodontic Failure:

Only one of the factors discussed above, intraradicular infection, can be managed by retreatment of the root canal.

  • Sjogren 1996 - The success rate of retreatment may reach approximately two-thirds of cases
  • Gutmann & Harrison 1991 and Lopes & Siqueira 1999Periradicular surgery is indicated in the following cases: the treatment or retreatment is impossible (fractured instruments, ledges, blockages, filling material impossible to remove); failure of retreatment; where the prognosis of the nonsurgical retreatment is unfavorable; where biopsy is needed.

 

 

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Week of November 26, 2001

 

 


Title: Laser doppler flowmetry for monitoring traumatized teeth.

Author: Lee, et.al

Journal: Dental Traumatology, 17 (5):231 October, 2001

Reviewer: Pranav Vohra, D.M.D.

Purpose: To report the case of using laser Doppler Flowmetry (LDF) for monitoring traumatized teeth.

Report: The case is of a 7 y.o., male with non-contributory medical history, who presented with a 4 mm laterally luxated #9, presenting 16 hours after a football accident.

Patient was anesthetized with 3% prolocaine w/out epi to maintain vasculature to the teeth.

The teeth were repositioned using a soft orthodontic arch wire, and patient placed on chlorhexidine twice a day and penicillin VK 250 mg for 7 days.

At the 10th day recall, the patient was asymptomatic, and healing was uneventful, so the splint was removed.

Vitality testing:

Tooth

Cold

Palp

Perc

7

N

N

N

8

N

N

N

9

NR

N

N

10

N

N

N

It was determined that to maintain the possibility of survival of vasculature, the teeth be monitored using LDF.

At two weeks post op there were no changes in the vitality testing by cold, and LDF results were noted.

At 3 months, #9 was asymptomatic and unresponsive to cold, with #7, 8, 9 being WNL. Radiographically, root development was seen in all four incisors, and the LDF of #9 showed an increasing flux value of 8 A.U.

At the six-month recall, all teeth were developing normally, and #9 showed a delayed response to cold, and a metallic sound to percussion indicating possible ankylosis.

At, 12 months, all teeth responded normally to cold and LDF on #9 showed a reading of 9A.U. All incisors responded normally to palpation and percussion with 2-3 mm probings, and a metallic percussion sound still evident on #9.

Discussion: According to the authors, the technique of using LDF on traumatized teeth gives a much more reliable way of monitoring vitality as compared to the more traditional tests like Endo Ice.

 

 


Title:  Guidelines for the evaluation and management of traumatic dental injuries Part II

Journal: Dental Traumatology 2001;17 97-102

Reviewer:  Nima Dayani, D.D.S.

Purpose:  This report includes basic information on both prevention and first aid after dental trauma to permanent teeth.

Table 1.  Treatment guidelines for tooth  and bone fractures in the permanent dentition

 

Crown fracture

Crown-root fracture

Root fracture

Alveolar fracture

Uncomplicated

Complicated

Diagnosis
Clinical findings

Enamel fracture or Enamel-dentin (E/D) fracture

Same as E/D fracture, with pulp exposure

The coronal fragment is attached to the gingiva and mobile.  The pulp may or may not be exposed.
The apical segment is usually not displaced.

Tooth is usually mobile and sometimes displaced

The bone segment containing the involved tooth/teeth is mobile

Radiographic assessment and findings

Take one radiograph (2)
Evaluate size of pulp chamber and stage of root development
Sensibility test

Take one radiograph (2)
Evaluate size of pulp chamber and stage of root development
Sensibility test

Take one radiograph (2)
The oblique fracture line is usually perpendicular to the central X-ray beam
Sensibility test

Take four radiographs (1-4).  Extra radiographs taken with different angulations can be useful.
Sensibility test

Take four radiographs (1-4).  Extra radiographs taken with different angulations can be useful.
Sensibility test

Treatment

Account for fractured segment.
Radiograph soft tissue lacerations for tooth fragments or other foreign bodies.   Provide a temporary glass-ionomer cement bandage or a permanent restoration using a bonding agent and composite resin
If very close to pulp, consider Ca(OH)2 base.  If an intact fragment exists, a bonding procedure may be carried out

In immature tooth:  Perform pulp capping or partial pulpotomy with Ca(OH)and bacteria tight coronal seal
In mature tooth:  As with immature tooth or Pulpectomy

In an emergency, stabilize the coronal fragment with an  acid etch/resin splint to adjacent teeth. Expose subgingival fracture site by:
a)  Gingivectomy
b) Orthodontic or surgical extrusion
If root formation is complete, root canal treatment with gutta percha/sealer indicated.
Otherwise, pulp capping or pulpotomy and wait for completion of root formation

Reposition the coronal fragment as soon as possible.  Check position radiographically.  Immobilize the tooth with a splint.

Reposition the fragment.  Splint the fragment to adjacent teeth with a splint

Patient instructions

Soft diet for 10-14 days
Brush teeth with a soft toothbrush after each meal
Use chlorhexidine mouthrinse (0.1%) twice a day for 7 days
Follow up (see Table 2)

(1) occlusal
(2) periapical central angle
(3) periapical mesial excentric
(4) periapical distal excentric

Table 2. Follow-up procedures for traumatized permanent teeth

Time

Crown fracture

Root fracture

Alveolar fracture

Uncomplicated

Complicated

1 week

 

 

 

 

2 - 3 weeks

 

 

 

 

3 - 4 weeks

 

 

S + C (2)

S + C (3)

6 - 8 weeks

C (1)

C (1)

C (2)

C (3)

6 months

 

 

C (2)

C (3)

1 year

C (1)

C (1)

C (2)

C (3)

 

 

 

C (2)

C (3)

 

S = Splint removal

C = Clinical and radiographic examination

(1)

Success - positive sensitivity, root continues development (immature teeth).  Continue to next evaluation

 

Failure - negative sensitivity, signs of apical periodontitis, root does not continue development (immature teeth).  Start endodontic therapy.

(2)

Success - positive sensitivity (false negative possible at 3-4 week evaluation).  Signs of repair of fractured segments.   Continue to next evaluation.

 

Failure - negative sensitivity (false negative possible at 3-4 week evaluation).  Clinical signs of periodontitis.   Radiolucency adjacent to fracture line.  Start endodontic therapy to level of fracture line.

(3)

Success - positive sensitivity (false negative possible at 3-4 week evaluation).  No signs of apical periodontitis.   Continue to next evaluation.

 

Failure - negative sensitivity (false negative possible at 3-4 week evaluation).  Signs of apical or external inflammatory resorption.  Start endodontic therapy.

 

 

 


Title:  Guidelines for the evaluation and management of traumatic dental injuries

Author:  Flores MT, Andreasen JO, Bakland LK, et al

Journal: Dental Traumatology, 17(5):193, October 2001

Reviewer: Joe Simoneaux, DDS

Purpose:  To develop guidelines for the treatment of traumatic dental injuries

Introduction:  Studies indicate that 50% of children (most often 8-12 y/o) sustain a traumatic dental injury. Crown fracture occurs most frequently as a result of accident, sport activity or violence. Appropriate treatment given in a timely manner can lessen the oral health and aesthetic impact. Due to new technology and improved understanding of the inflammatory process, there is now a more conservative approach in handling dental trauma. The International Association of Dental Traumatology had developed the following guidelines as a consensus statement for the variation in trauma treatment. These guidelines are helpful in assisting dentists and other healthcare providers in delivering the best care in the most efficient manner, thus, improving the short and long-term outcome. Although it is beyond the scope of these recommendations, major facial trauma of the bone and soft tissue is the critical first step in the overall management of maxillofacial trauma.

Table 1.  Treatment guidelines for avulsed teeth with closed apex

Diagnosis
Clinical situation

The tooth has already been replanted

The tooth has been kept in special storage media, milk, saline or saliva.
The extra-oral dry time is less than 60 min

Extra-oral dry time > 60 min

Treatment

Clean affected area with water spray, saline or chlorhexidine.
Do not extract the tooth

If contaminated, clean the root surface and apical foramen with a stream of saline.
Remove the coagulum from the socket with a stream of saline.
Examine the alveolar socket.  If there is a fracture in the socket wall, reposition it with a suitable instrument.
Replant slowly with slight digital pressure.

Remove debris and necrotic periodontal ligament.
Remove the coagulum from the socket with a stream of saline.  If there is a fracture in the socket wall, reposition it with a suitable instrument.
Immerse the tooth in a 2.4% sodium fluoride solution acidulated to a pH 5.5 for a minimum of 5 min or, if available, fill the socket with Emdogain®
Replant slowly with slight digital pressure.

 

Suture gingival laceration, especially in the cervical area
Verify normal position of the replanted tooth radiographically
Apply a flexible splint for 1 week

 

Administer systemic antibiotics:   Doxycycline 2x per day for 7 days at appropriate dose for patient age and weight
Refer to physician to evaluate need for a tetanus booster if avulsed tooth has come into contact with soil or tetarus coverage is uncertain
Initiate endodontic treatment after 7-10 days. Place calcium hydroxide as an intra-canal medicament

Patient instruction

Soft diet for 2 weeks
Brush teeth with a soft toothbrush after each meal
Use a chlorhexidine mouthrinse (0.1%) twice a day for 1 week
Follow-up (See Table 3)


Table 2. Treatment guidelines for avulsed teeth with open apex

Diagnosis
Clinical situation

The tooth has already been replanted

The tooth has been kept in special storage media, milk, saline or saliva.
The extra-oral dry time is less than 60 min

Extra-oral dry time > 60 min

Treatment

Clean affected area with water spray, saline or chlorhexidine.
Do not extract the tooth

If contaminated, clean the root surface and apical foramen with a stream of saline.
Place the tooth in doxycycline (~1mg/20ml saline).
Remove the coagulum from the socket with a stream of saline.
Examine the alveolar socket.  If there is a fracture in the socket wall, reposition it with a suitable instrument.
Replant slowly with slight digital pressure.

Replantation is not indicated

 

Suture gingival laceration, especially in the cervical area
Verify normal position of the replanted tooth radiographically
Apply a flexible splint for 1 week

 

Administer systemic antibiotics:   Penicillin V 1000mg and 500 mg 4x per day for 7 days or for patients not susceptible to tetracycline staining, Doxycycline 2x per day for 7 days at appropriate dose for patient age and weight
Refer to physician to evaluate need for a tetanus booster if avulsed tooth has come into contact with soil or tetarus coverage is uncertain

Patient instruction

Soft diet for 2 weeks
Brush teeth with a soft toothbrush after each meal
Use a chlorhexidine mouthrinse (0.1%) twice a day for 1 week
Follow-up (See Table 3)

Table 3.  Follow-up procedures for traumatized permanent teeth

Time

Closed apex

Open apex

1 week

S
Intitiate endodontic treatment

S
Intitiate endodontic treatment

2-3 weeks

C

C

3-4 weeks

C

C

6-8 weeks

C

C

6 months

C

C

1 year

C

C

5 years

C

C

 

S = Splint removal       C = Clinical and radiographic examination

 

Closed Apex

(1) Satisfactory outcome -

Clinical:  asymptomatic, normal mobility, normal sound on percussion.
Radiographic:  no periradicular radiolucencies indicative of progressive external inflammatory root resorption (>2x normal lamina dura) or loss of lamina dura indicative of ankylosis and replacement resorption.

(2) Unsatisfactory outcome -

Clinical:  symptomatic and/or high pitch percussion sound.
Radiographic:  periradicular radiolucencies in the root and bone or radiographic replacement of the root with bone.

Endodontic treatment:  At 7-10 days endodontic treatment should be initiated and calcium hydroxide placed.  Calcium hydroxide can be replaced by gutta percha if or when an intact lamina dura can be traced around the entire root surface.
Normally, if the root treatment is initiated at the end of the ideal 7-day period, external inflammatory root resorption is prevented and obturation can take place within a month.  If, however the endodontic treatment is initiated when root resorption is already visible, calcium hydroxide is needed for an extended period before obturation can take place.  The status of the lamina dura and the presence of the calcium hydroxide in the canal should be evaluated every 3 months.

Open Apex

(1) Satisfactory outcome -

Clinical:  asymptomatic, normal mobility, normal sound on percussion.  Positive sensitivity test.
Radiographic:  As with closed apex.  Continued root development, pulp lumen obliteration extremely common.

(2) Unsatisfactory outcome -

Clinical:  symptomatic and/or high pitch percussion sound.  Tooth in infra-occlusion.
Radiographic:  As with closed apex.  Root fails to develop, the pulpal lumen does not change in size.

Endodontic treatment:  If revascularization is a possibility, avoid endodontic treatment unless obvious signs of failure are present.   Sensitivity test may take up to 3 months to respond positively.  If endodontic treatment is necessary, follow recommendations for apexification.

 

 

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