Professor Allen Nicholson — Selected Publications

Meng, W. and Nicholson, A.W. 2007. Bacterial ribonuclease III heterodimer-based analysis of subunit and domain contributions to double-stranded RNA processing activity invitro." Biochemistry Journal.

Pertzev, AV., Nicholson, A.W. 2006. "Characterization of RNA sequence determinants and antideterminants of processing reactivity for a minimal substrate of Escherichia coli ribonuuclease III." Nucleic Acids Res. 34: 3708-3721.

Sun, W., Pertzev, A., and Nicholson, A.W. 2005. "Catalytic mechanism of Escherichia coli ribonuclease III.  Kinetic and inhibitor evidence for the involvement of two magnesium ions in RNA phosphodiester hydrolysis."  Nucleic Acids Res. 33: 807-815.

Sun, W., Li, G. and Nicholson, A.W. 2004. "Mutational analysis of the nuclease domain of Escherichia coli ribonuclease III. Identification of conserved residues that are important for catalytic function in vitro." Biochem. 43: 13054-13062.

Zhang, Y., Calin-Jageman, I., Gurnon, J.R., Choi, T.J., Adams, B., Nicholson, A.W. and Van Etten, J.L. 2003. "Characterization of a Chlorella virus PBCV-1 encoded ribonuclease III.  Virol. 317: 73-83.

Nicholson, A. W. 2003. "The ribonuclease III superfamily: forms and functions in RNA maturation, decay, and gene silencing." In: "RNAi: A Guide to Gene Silencing" (G. Hannon, Ed) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY .

Calin-Jageman, I. and Nicholson, A. W. 2003. "Mutational analysis of an RNA internal loop as a reactivity epitope for Escherichia coli ribonuclease III substrates." Biochem. 42: 5025-5034.

Calin-Jageman, I. and Nicholson, A. W. 2003. "RNA structure-dependent uncoupling of substrate binding and cleavage by Escherichia coli ribonuclease III." Nucleic Acids Res. 31: 2381-2392.

Zhang, Y., Calin-Jageman, I., Gurnon, J. R., Adams, B., Nicholson, A. W., and Van Etten, J. L. 2003. "Characterization of a Chlorella virus PBCV-1 encoded ribonuclease III." Virology (in press).

Fortin, K. R., Nicholson, R.H. and Nicholson, A. W. 2002. "Mouse ribonuclease III: cDNA structure, expression analysis, and chromosomal location." BMC Genomics: 3:26.

Nicholson, R. H. and Nicholson, A. W. 2002. "Characterization and expression analysis of a mouse cDNA encoding Dicer, a ribonuclease III orthologue involved in RNA interference." Mamm. Genome 13:67-73.

Amarasinghe, A., Calin-Jageman, I., Harmouch, A., Sun, W. and Nicholson, A. W. 2001. Escherichia coli ribonuclease III. Purification of hexahsitidine-tagged enzyme, and assays for substrate binding and cleavage." Methods Enzymol. 342, 143-158.

Marchand, I., Nicholson, A. W. and Dreyfus, M. 2001. "Bacteriophage T7 protein kinase phosphorylates RNase E and stabilizes mRNAs synthesized by T7 RNA polymerase." Molecular Microbiology 42: 767-776.

Sun, W., Jun, E. and Nicholson, A. W. 2001. "Intrinsic double-stranded RNA processing activity of Escherichia coli ribonuclease III lacking the double-stranded RNA-binding domain." Biochemistry 40: 14976-14984.

Calin-Jageman, I., Amarasinghe, A. and Nicholson, A. W. 2001. "Ethidium-dependent uncoupling of RNA binding and cleavage by Escherichia coli ribonuclease III." Nucleic Acids Res. 29: 1915-1925.

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