Assistant Professor

B.S. (2000) University of Arizona

Ph.D. (2005) University of Michigan

Postdoctoral Fellow (2005-2007) National Institute of Standards & Technology

OFFICE
Beury Hall 240

MAILING ADDRESS

Department of Chemistry
Beury Hall 130
1901 N. 13th Street
Philadelphia, PA 19122

PHONE
office: 215-204-1973
fax: 215-204-1532

E-MAIL

jshackman@temple.edu

RESEARCH GROUP

http://astro.temple.edu/~shackman/

Analytical / Medicinal / Photonics

Research Interests

Recent developments in microfabrication technologies have opened up many possible avenues for revolutionary research in a wide range of fields and have found wide spread application in the chemical and biological sciences.   Miniaturization strategies frequently seek to encapsulate all processes of analysis, including sample preparation, separations, detection, and data analysis.   Capillary electrophoresis (CE) is a powerful analytical tool that is commonly utilized in these microfluidic devices to perform the separation of analytes.   While affording rapid, high resolution separations with small sample requirements, CE suffers from low concentration limits of detection (LOD) when using the most common optical detection techniques ( i.e. , absorbance and fluorescence).   The detection problem has been exacerbated when translated from fused silica capillaries to microdevices, which frequently employ thick borosilicate glass or polymeric materials, as well as extremely small channel dimensions, that further degrade LOD.   While alternative detection strategies and microcolumn geometries have been employed to improve LOD, sample enrichment methods, both preconcentration and in-line concentration, have also been very successfully utilized to bring analytes up to the detection limits of the common detectors using standard capillary instruments.

Our research places heavy emphasis on electrophoretic sample enrichment techniques for CE and microfluidics to perform the trace analysis requirements of bioanalytical problems.   A major area of investigation is utilization of electrophoretic focusing methods, which incorporate simultaneous separation and concentration enhancement, for trace measurements using more universal detectors than fluorescence ( e.g. , UV absorbance, Raman spectroscopy, etc. ), which also mitigate the need for analyte labeling prior to detection.   A second area of interest is evaluating the efficacy of counter-flow electrophoretic separations for isolating and quantifying analytes from complex mixtures, such as from biological fluids, with little or no sample preparation.   Additionally, multi-dimensional combinations of the techniques are being investigated for performing both trace analyses and complex composition separations.   All methods are being developed with the intent of implementation in microfluidic formats to promote integration, transferability, and automation for biochemical monitoring.

Selected Publications

"Finite sample effect in temperature gradient focusing." J.G. Shackman, H. Lin, and D. Ross. Lab on a Chip , 8 (2008) , 969-978.

"Gradient elution isotachophesis with direct ultraviolet absorption detection for sensitive amino acid analysis." J.G. Shackman, M. Mamunooru, R.J. Jenkins, and N.I. Davis. Journal of Chromatography A , 1202 (2008) , 203-211.

"Temperature gradient focusing for microchannel separations." J.G. Shackman, M.S. Munson, and D. Ross. Analytical and Bioanalytical Chemistry , 387 (2007) , 155-158.

“Counter-flow Gradient Electrofocusing.” J.G. Shackman and D. Ross.   Electrophoresis , 28 (2007) , 556-571.

“Gradient Elution Moving Boundary Electrophoresis for High-Throughput Multiplexed Microfluidic Devices.”   J.G. Shackman, M.S. Munson, and D. Ross.   Analytical Chemistry , 79 (2007) , 565-571.

“Quantitative Temperature Gradient Focusing Performed Using Background Electrolytes at Various pH Values.”   J.G. Shackman, M.S. Munson, C-W. Kan, and D. Ross.   Electrophoresis , 27 (2006) , 3420-3427.

“Total Insulin and IGF-I Resistance in Pancreatic Beta Cells Causes Overt Diabetes.”   K. Ueki, T. Okada, J. Hu, C.W. Liew, A. Assmann, G.M. Dahlgren, J.L. Peters, J.G. Shackman, M. Zhang, I. Artner, L.S. Satin, R. Stein, M. Holzenberger, R.T. Kennedy, C.R. Kahn, and R.N. Kulkarni.   Nature Genetics , 38 (2006) , 583-588.

“Microfluidic Electrophoresis Chip Coupled to Microdialysis for In Vivo Monitoring of Amino Acid Neurotransmitters.”   Z.D. Sandlin, M.S. Shou, J.G. Shackman, and R.T. Kennedy.   Analytical Chemistry , 77 (2005) , 7702-7708.

“Perfusion and Chemical Monitoring of Living Cells on a Microfluidic Chip.”   J.G. Shackman, G.M. Dahlgren, J.L. Peters, and R.T. Kennedy.   Lab on a Chip , 5 (2005) , 56-63.

“ In Vivo Monitoring of Amino Acids by Microdialysis Sampling with On-Line Derivatization by Naphthalene-2,3-Dicarboxyaldehyde and Rapid Micellar Electrokinetic Capillary Chromatography.”   M. Shou, A.D. Smith, J.G. Shackman, J. Peris, and R.T. Kennedy.   Journal of Neuroscience Methods , 138 (2004) , 189-197.

“High-Throughput Automated Post-Processing of Separation Data.”   J.G. Shackman, C.J. Watson, and R.T. Kennedy.   Journal of Chromatography A , 1040 (2004) , 273-282.

“Microfluidic Chip for Continuous Monitoring of Hormone Secretion from Live Cells using an Electrophoresis-Based Immunoassay.”   M.G. Roper, J.G. Shackman, G.M. Dahlgren, and R.T. Kennedy.   Analytical Chemistry , 75 (2003) , 4711-4717.

“Pharmaceutical Reaction Monitoring by Raman Spectroscopy.”   J.G. Shackman, J.H. Giles, and M.B. Denton.   In Further Developments in Scientific Optical Imaging .   The Royal Chemical Society: Cambridge 2000 ; pp 186-201.